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Erschienen in: Archives of Virology 2/2018

02.11.2017 | Original Article

Serodiagnosis, targeting nonstructural protein 4, of porcine reproductive and respiratory syndrome virus

verfasst von: Shengliang Cao, Xinna Cai, Min Tan, Fachao Sun, Yuchao Wang, Yingli Shang, Sidang Liu, Yihong Xiao

Erschienen in: Archives of Virology | Ausgabe 2/2018

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Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) causes great economic losses to the swine industry worldwide. Typically, an N protein-coated indirect enzyme-linked immunosorbent assay (N-coated iELISA) is used to detect PRRSV antibodies. Non-structural protein (NSP) 4 is essential to the PRRSV life cycle and contains B-cell epitopes. Yet, no specific antibody against NSP4 has been detected in clinical samples. In this study, we developed an NSP4-coated iELISA and compared its effectiveness with the N-coated iELISA. The NSP4-coated iELISA was developed with a cut-off value of 0.406 at an optical density of 450 nm by testing a panel of 70 PRRSV positive and 80 PRRSV negative pig serum samples, which generated a specificity and sensitivity of 100%. Agreement between the NSP4-coated and N-coated iELISAs was 92.2%. Interestingly, 50 serum samples, mostly from pigs vaccinated with the HP-PRRSV live strain, tested positive for PRRSV antibodies with the NSP4-coated iELISA, but were negative with the N-coated iELISA. These results were further confirmed by western blot analysis and another iELISA based on the N-terminus of NSP2 (NSP2-1-coated iELISA). The agreement between the results of western blot analysis with the NSP4-coated and NSP2-1-coated iELISA analyses were 92% and 96.1%, respectively, showing that the developed NSP4-coated iELISA is a useful tool to discriminate a false negative from a true negative response to the HP-PRRSV vaccine.
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Metadaten
Titel
Serodiagnosis, targeting nonstructural protein 4, of porcine reproductive and respiratory syndrome virus
verfasst von
Shengliang Cao
Xinna Cai
Min Tan
Fachao Sun
Yuchao Wang
Yingli Shang
Sidang Liu
Yihong Xiao
Publikationsdatum
02.11.2017
Verlag
Springer Vienna
Erschienen in
Archives of Virology / Ausgabe 2/2018
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-017-3625-5

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