Background
Methods
Subjects
Clinical/demographic | Autism (n = 55) | Control (n = 55) | P value |
---|---|---|---|
Age | 11.29 ± 2.45 (6 to 16) | 11.31 ± 2.37 (6 to 16) | 0.9685a |
Sex | |||
Male | 48 | 41 | 0.089b |
Female | 7 | 14 | |
ADI-R | |||
Domain A score, social | 17.7 ± 7.62 (10 to 29) | ||
Domain BV score, communication | 12.8 ± 5.62 (8 to 25) | ||
Domain C score, stereotype | 4.1 ± 2.84 (3 to 12) |
Serum separation
RNA extraction and cDNA synthesis
microRNA screening
Normalization
Data analysis
Quantitative reverse-transcription PCR
Normalization
Statistical analysis
Enrichment pathway analysis and target gene prediction
Results
microRNA screening
Confirmation with quantitative PCR
Receiver operating characteristic analysis
Enrichment pathway analysis and target gene prediction
Discussion
miR ID | Present result | Previous report | Type of sample | Reference |
---|---|---|---|---|
hsa-miR-106b-5p | ↑ | ↓ | Brain | [13] |
hsa-miR-181b-5p | ↓ | ↓ | Brain, lymphoblastoid cell line | |
hsa-miR-195-5p | ↑ | ↑ | Lymphoblastoid cell line | [15] |
hsa-miR-19b-3p | ↑ | ↓ | Lymphoblastoid cell line | [14] |
hsa-miR-320a | ↓ | ↓ | Lymphoblastoid cell line | [14] |
hsa-miR-328 | ↓ | ↓ | Brain | [13] |
hsa-miR-663a | ↓ | ↑ | Lymphoblastoid cell line | [14] |