Background
Methods
Cell culture, siRNA, and plasmid transfection
Clinical samples
Immunohistochemistry and scoring
Detection of EGFR status
Biostatistics mining of NCBI database
Western blotting
Reverse transcription and quantitative real-time PCR
Rho GTPase activation assays
Invasion and migration assays
Results
SGEF is over-expressed in lung cancer
SGEF (low) | SGEF (high) | P value | |
---|---|---|---|
Carcinoma | 38 | 54 | P < 0.001 |
Adjacent normal tissue | 72 | 15 |
High SGEF is closely related with advanced TNM stage and poor prognosis in LAC
WHO grade | |||
---|---|---|---|
Feature | I (n = 11) | II (n = 73) | III (n = 8) |
Gender | |||
Male | 7 | 38 | 6 |
Female | 4 | 35 | 2 |
Age at diagnosis | |||
<60 | 5 | 25 | 3 |
≥ 60 | 6 | 48 | 5 |
T stage | |||
T1–2 | 11 | 54 | 4 |
T3–4 | 0 | 19 | 4 |
N stage | |||
N0 | 9 | 34 | 4 |
N1–3 | 2 | 39 | 4 |
TNM stage | |||
I | 8 | 25 | 2 |
II | 3 | 22 | 2 |
III | 0 | 26 | 4 |
Location | |||
Left | 3 | 26 | 4 |
Right | 8 | 47 | 4 |
SGEF | |||
---|---|---|---|
Feature | High (n = 54) | Low (n = 38) | P value |
Gender | P = 0.192 | ||
Male | 33 (61.11%) | 18 (47.37%) | |
Female | 21 (28.89%) | 20 (52.63%) | |
Age at diagnosis | P = 0.781 | ||
<60 | 20 (37.04%) | 13 (34.21%) | |
≥ 60 | 34 (62.96%) | 25 (65.79%) | |
Location | P = 0.407 | ||
Left | 21 (38.89%) | 12 (31.58%) | |
Right | 33 (61.11%) | 26 (68.42%) | |
EGFR status | P = 0.545 | ||
Mutant | 18 (33.33%) | 15 (39.47%) | |
Wild type | 36 (66.67%) | 23 (60.53%) | |
T stage | P = 0.028 | ||
T1–2 | 36 (66.66%) | 33 (86.84%) | |
T3–4 | 18 (33.34%) | 5 (13.16%) | |
N stage | P = 0.011 | ||
N0 | 21 (38.88%) | 25 (65.79%) | |
N1–3 | 33 (61.12%) | 13 (34.21%) | |
TNM stage | P < 0.001 | ||
I | 7 (12.96%) | 18 (47.36%) | |
II + III | 47 (87.04%) | 20 (52.63%) | |
Histological grade | P = 0.608 | ||
Well | 6 (11.11%) | 5 (11.36%) | |
Moderate | 42 (77.78%) | 31 (70.45%) | |
Poor | 6 (11.11%) | 2 (4.54%) |
Factors | Univariate | Multivariate | ||
---|---|---|---|---|
HR (95% CI) | P value | HR (95% CI) | P value | |
Gender | 0.666 (0.379–1.169) | 0.156 | 0.578 (0.321–1.042) | 0.069 |
Age | 1.019 (0.996–1.042) | 0.839 | 1.045 (1.010–1.081) | 0.011 |
Location | 0.993 (0.527–1.651) | 0.811 | 1.127 (0.587–2.164) | 0.718 |
SGEF expression | 1.846 (1.029–3.310) | 0.040 | 1.126 (0.631–2.382) | 0.547 |
Grade | 2.474 (1.351–4.532) | 0.003 | 2.337 (1.177–4.643) | 0.015 |
TNM stage | 1.603 (1.159–2.217) | 0.004 | 1.532 (1.052–2.202) | 0.026 |