The SHL injection, prepared according to the Chinese Pharmacopoeia [
13], was provided by Duoduo Pharmaceutical Co., Ltd. (Jiamusi, Heilongjiang, China). Pluonic F-127 and active papain were from Sigma-Aldrich (St Louis, MO, USA). Fluo-3 AM Ester was from Biotium (San Francisco, CA, USA). Mouse total IgE (tIgE), IL-4, IL-5 and IL-10 ELISA kits were from Biolegend Co. (San Diego, CA, USA). Mouse IL-13 and IFN-γ ELISA kits were from Excell Bio. Co. (Shanghai, China). Recombinant mouse IL-3 was from Novoprotein (Summit, NJ, USA). Mouse direct lineage cell depletion kit, CD3-FITC antibody, CD117-APC antibody, CD19-VioBright (TM) FITC antibody, CD117 Microbeads, FcεRI-PE antibody, anti-PE Microbeads and Red Blood Cell Lysis solution were from Miltenyi Biotec Inc. (Auburn, CA, USA). Protein G PLUS-Agarose was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). The HRP-labeled rat anti-mouse IgE antibody and HRP-labeled goat anti-rabbit antibody were from Abcam Co. (Cambridge, UK) and Jackson ImmunoResearch Laboratories Inc. (Lancaster, PA, USA), respectively. The rabbit anti-mouse IgG
2a and IgG
2b antibodies were from OriGene Technologies, Inc. (Rockville, MD, USA). The nuclear factor of activated T cells (NFAT)-luc plasmid and its control were synthesized by GenePharma Co., Ltd. (Shanghai, China). The FITC-labeled anti-mouse IgE antibody and PE-labeled anti-mouse CD200R antibody were from BD Biosciences (Franklin Lakes, NJ, USA) and eBioscience (San Diego, CA, USA), respectively. The luciferase assay system was from Beyotime Institute of Biotechnology (Haimen, Jiangsu, China). Imject alum adjuvant was from Thermo Fisher Scientific (New York, NY, USA). The other reagents were of analytical grade.