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Erschienen in: European Journal of Nutrition 8/2012

01.12.2012 | Original Contribution

Silk and silkworm pupa peptides suppress adipogenesis in preadipocytes and fat accumulation in rats fed a high-fat diet

verfasst von: Sun Hee Lee, Dongsun Park, Goeun Yang, Dae-Kwon Bae, Yun-Hui Yang, Tae Kyun Kim, Dajeong Kim, Jangbeen Kyung, Sungho Yeon, Kyo Chul Koo, Jeong-Yong Lee, Seock-Yeon Hwang, Seong Soo Joo, Yun-Bae Kim

Erschienen in: European Journal of Nutrition | Ausgabe 8/2012

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Abstract

Purpose

The objective was to confirm the anti-obesity activity of a silk peptide (SP) and a silkworm pupa peptide (SPP) in rats fed a high-fat diet (HFD) and to elucidate their action mechanism(s) in a preadipocyte culture system.

Methods

In an in vitro mechanistic study, the differentiation and maturation of 3T3-L1 preadipocytes were stimulated with insulin (5 μg/mL), and effects of SP and SPP on the adipogenesis of mature adipocytes were assessed. In an in vivo anti-obesity study, male C57BL/6 mice were fed an HFD containing SP or SPP (0.3, 1.0, or 3.0%) for 8 weeks, and blood and tissue parameters of obesity were analyzed.

Results

Hormonal stimulation of preadipocytes led to a 50–70% increase in adipogenesis. Polymerase chain reaction and Western blot analyses revealed increases in adipogenesis-specific genes (leptin and Acrp30) and proteins (peroxisome proliferator-activated receptor-γ and Acrp30). The hormone-induced adipogenesis and activated gene expression was substantially inhibited by treatment with SP and SPP (1–50 μg/mL). The HFD markedly increased body weight gain by increasing the weight of epididymal and mesenteric fat. Body and fat weights were significantly reduced by SP and SPP, in which decreases in the area of abdominal adipose tissue and the size of epididymal adipocytes were confirmed by magnetic resonance imaging and microscopic examination, respectively. Long-term HFD caused hepatic lipid accumulation and increased blood triglycerides and cholesterol, in addition to their regulatory factors Acrp30 and leptin. However, SP and SPP recovered the concentrations of Acrp30 and leptin, and attenuated steatosis.

Conclusions

SP and SPP inhibit the differentiation of preadipocytes and adipogenesis by modulating signal transduction pathways and improve HFD-induced obesity by reducing lipid accumulation and the size of adipocytes.
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Metadaten
Titel
Silk and silkworm pupa peptides suppress adipogenesis in preadipocytes and fat accumulation in rats fed a high-fat diet
verfasst von
Sun Hee Lee
Dongsun Park
Goeun Yang
Dae-Kwon Bae
Yun-Hui Yang
Tae Kyun Kim
Dajeong Kim
Jangbeen Kyung
Sungho Yeon
Kyo Chul Koo
Jeong-Yong Lee
Seock-Yeon Hwang
Seong Soo Joo
Yun-Bae Kim
Publikationsdatum
01.12.2012
Verlag
Springer-Verlag
Erschienen in
European Journal of Nutrition / Ausgabe 8/2012
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI
https://doi.org/10.1007/s00394-011-0280-6

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