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Erschienen in: Journal of Artificial Organs 2/2020

03.11.2019 | Original Article

Simple and efficient method for consecutive inactivation–cryopreservation of porcine skin grafts

verfasst von: Hiromu Masuoka, Naoki Morimoto, Atsushi Mahara, Michiharu Sakamoto, Toshihito Mitsui, Natsuko Kakudo, Kenji Kusumoto, Tetsuji Yamaoka

Erschienen in: Journal of Artificial Organs | Ausgabe 2/2020

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Abstract

We previously reported that inactivation treatment by high hydrostatic pressurization (HHP) has potential utility as a novel skin regeneration therapy for various skin tumors. In this study, we evaluated whether glycerol-cryopreservation could be applied in order to preserve inactivated skin by HHP using a porcine model. Twenty full-thickness skin grafts (1.5 × 1.5 cm) were prepared from a minipig. The skin samples were inactivated by the HHP in normal saline or glycerol/fructose solution, followed by cryopreservation for 5 weeks at − 80 °C in each same solution. Another 10 grafts immediately after inactivation were prepared as non-cryopreserved controls. Nine grafts in each group were randomly implanted on the fascia of a host pig and removed at 1, 4 and 11 weeks after grafting. All grafts showed engraftment macroscopically. Hematoxylin eosin staining showed the cellular components in all areas of the dermis at 4 and 11 weeks after grafting, and immunohistochemical staining for CD31 showed the presence of capillaries in the grafts in all groups. The surface and cross-sectional areas of grafts in the normal saline solution cryopreserved group decreased between 1 and 11 weeks, whereas these areas in the glycerol cryopreserved group did not decrease significantly. Glycerol cryopreservation may therefore be a simple and efficient method for preserving porcine skin inactivated by HHP.
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Metadaten
Titel
Simple and efficient method for consecutive inactivation–cryopreservation of porcine skin grafts
verfasst von
Hiromu Masuoka
Naoki Morimoto
Atsushi Mahara
Michiharu Sakamoto
Toshihito Mitsui
Natsuko Kakudo
Kenji Kusumoto
Tetsuji Yamaoka
Publikationsdatum
03.11.2019
Verlag
Springer Japan
Erschienen in
Journal of Artificial Organs / Ausgabe 2/2020
Print ISSN: 1434-7229
Elektronische ISSN: 1619-0904
DOI
https://doi.org/10.1007/s10047-019-01142-2

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