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01.12.2017 | Case report | Ausgabe 1/2017 Open Access

Pediatric Rheumatology 1/2017

Simultaneous development of Kawasaki disease following acute human adenovirus infection in monozygotic twins: A case report

Zeitschrift:
Pediatric Rheumatology > Ausgabe 1/2017
Autoren:
Sayaka Fukuda, Shuichi Ito, Maya Fujiwara, Jun Abe, Nozomu Hanaoka, Tsuguto Fujimoto, Hiroshi Katsumori
Abbreviations
ALT
Alanine aminotransferase
AST
Aspartate aminotransferase
CRP
C-reactive protein
G-CSF
Granulocyte colony-stimulating factor
HAdV
Human adenovirus
HAdV-3
Human adenovirus type 3
IFN-γ
Interferon-γ
IL-18
Interleukin-18
IL-6
Interleukin-6
IL-8
Interleukin-8
IVIG
Intravenous immunoglobulin
KD
Kawasaki disease
sTNFR-1
Soluble tumor necrosis factor receptor 1
sTNFR-2
Soluble tumor necrosis factor receptor 2
TNF-α
Tumor necrosis factor α
WBC
White blood cells

Background

Kawasaki disease (KD) is an acute systemic vasculitis syndrome, first reported by Dr. Tomisaku Kawasaki in 1967 [1]. Although its etiology remains unknown, specific genetic factors and/or some infectious agents may underlie the onset of KD [2, 3]. Previous studies have shown that human adenovirus (HAdV) might be one of the pathogens that triggers KD [4]. Here, we describe a case report of monozygotic twins who simultaneously developed KD after an acute HAdV type 3 (HAdV-3) infection, which was confirmed by PCR-sequencing, virus isolation, and seroconversion. Our case is the first report of KD associated with monozygotic twins who suffered a HAdV-3 infection, and it supports the hypothesized pathogenesis described above.

Case presentation

The patients were four-year-old monozygotic twin boys who had previously been healthy and had no family history of KD.

Case 1: Elder brother

The elder brother developed a high fever on day 1 of his illness and subsequently developed redness of the eyes, red cracked lips, strawberry tongue, erythema, swollen red palms and soles, and cervical lymphadenopathy. He was admitted to our hospital on the seventh day after fever onset. On admission, he met all six diagnostic criteria for KD and was also diagnosed with HAdV infection with a rapid test for AdV (Imunoace®adeno, TAUNS Laboratories Inc. Shizuoka, Japan). His laboratory data were: white blood cells (WBC), 9700/μL (neutrophil sequestration, 66.0%); hematocrit, 38.8%; platelet count, 35.7 × 104/μL; albumin, 4.1 g/dL; total bilirubin, 0.5 mg/dL; sodium, 130 mEq/L; aspartate aminotransferase (AST), 31 IU/L; alanine aminotransferase (ALT), 16 IU/L; and C-reactive protein, (CRP) 2.4 mg/dL. His serum cytokine profile was: granulocyte-colony stimulating factor (G-CSF), 384.0 pg/mL; interferon-γ (IFN-γ), 99.8 pg/mL; interleukin-6 (IL-6), 43.8 pg/mL; IL-8, 36.9 pg/mL; IL-18, 1200.6 pg/mL; tumor necrosis factor (TNF-α), 10.9 pg/mL; soluble tumor necrosis factor receptor 1 (sTNFR-1), 1106.9 pg/mL; and sTNFR-2, 10,013.4 pg/mL (Table 1). The patient was immediately treated with 2 g/kg intravenous immunoglobulin (IVIG) and aspirin. He responded well and achieved defervescence the next day, and all his symptoms disappeared promptly. Desquamation of the fingers was observed on day 12 of illness. He discharged on the 16th day of hospitalization (Fig. 1). A transthoracic echocardiography revealed no coronary artery abnormalities.
Table 1
Patient laboratory data on admission
 
(normal range)
Case 1
Case 2
WBC
/μL
 
9700
12,600
Neut.
%
 
66.0
71.0
Htc.
%
 
38.8
36.8
Plt.
×104/μL
 
35.7
39.2
Albumin
g/dL
 
4.14
4.33
Tbil.
mg/dL
 
0.5
0.6
Na
mEq/L
 
130.4
132.3
AST
IU/L
 
31
30
ALT
IU/L
 
16
13
CRP
mg/dL
 
2.42
5.54
G-CSF
pg/mL
(<100 pg/mL)
384.0
511.0
IFN-γ
pg/mL
(<30 pg/mL)
99.8
105.0
IL-6
pg/mL
(<20 pg/mL)
43.8
37.6
IL-8
pg/mL
(<30 pg/mL)
36.9
33.5
IL-18
pg/mL
(<500 pg/mL)
1200.6
580.4
TNFα
pg/mL
(<20 pg/mL)
10.9
33.6
sTNFR-1
pg/mL
(<500 pg/mL)
1106.9
736.4
sTNFR-2
pg/mL
(<5000 pg/mL)
10,013.4
8063.8
WBC white blood cells, Neut. neutrophil sequestration, Htc. hematocrit, Plt. platelet count, Tbil. total bilirubin, Na sodium, AST aspartate aminotransferase, ALT alanine aminotransferase, CRP C-reactive protein, G-CSF granulocyte colony-stimulating factor, IFN-γ interferon-γ, IL-6, -8, -18 interleukin-6, -8, -18, respectively, TNF-α tumor necrosis factor,
sTNFR-1, −-2 soluble tumor necrosis factor receptor 1, 2, respectively

Case 2: Younger brother

The younger brother also presented with a high fever and redness of eyes 4 days after his elder brother’s symptom onset, and he then developed red cracked lips, strawberry tongue, and cervical lymphadenopathy on day 3. The patient was hospitalized on the same day as his elder brother after experiencing a persistent fever for 3 days, and a rapid test for HAdV was positive at this time. His laboratory data upon admission were: WBC, 12,600/μL (neutrophil sequestration, 65.1%); hematocrit, 36.8%; platelet count, 39.2 × 104/μL; albumin, 4.3 g/dL; total bilirubin, 0.6 mg/dL; sodium, 132 mEq/L; AST, 30 IU/L; ALT, 13 IU/L; and CRP, 5.5 mg/dL. His serum cytokine levels were: G-CSF, 511.0 pg/mL; IFN-γ, 105.0 pg/mL; IL-6, 37.6 pg/mL; IL-8, 33.5 pg/mL; IL-18, 580.4 pg/mL; TNF-α, 33.6 pg/mL; sTNFR-1, 736.4 pg/mL; and sTNFR-2, 8063.8 pg/mL (Table 1). Unlike his brother, this patient met only three of the diagnostic criteria for KD upon admission, so we diagnosed him as having incomplete KD and did not treat him with IVIG or aspirin. On the day 5 of illness (2 days post-admission), the patient displayed all six symptoms of KD, but his fever spontaneously resolved on day 6 of illness without treatment (the patient received neither IVIG nor aspirin). After defervescence, all symptoms soon disappeared, and his laboratory data normalized. His CRP was 2.74 mg/dL on day 5 of illness and 0.22 mg/dL on day 10 of illness. On day 9 of illness, finger desquamation was observed. Unlike his brother, this patient’s echocardiography revealed a dilation of the left circumflex artery on day 10 of illness. We commenced treatment with 5 mg/kg/day of aspirin and 3 mg/kg/day of dipyridamole. He was discharged on the 16th day of hospitalization (Fig. 1). The maximum diameter of the coronary artery abnormality reached 3.9 mm (Z score, 4.7), but the coronary artery abnormality resolved within 3 months of onset.
Fecal samples were collected from both patients during the acute phase of the disease. These samples were tested by PCR assays, and HAdV-3 genomes were detected in the samples from both patients. Additionally, HAdV-3 was directly isolated from the younger brother’s fecal sample. Both patients were negative for serum neutralizing antibodies against HAdV-3 upon admission, but these antibodies were significantly elevated in both patients 2 weeks after their admission, suggesting that they underwent seroconversion. The patients’ father and elder sister also developed clinical symptoms consistent with acute HAdV infection around the time that the twins were infected.

Discussion

Here, we have presented a case of 4-year-old monozygotic twins who sequentially developed KD in conjunction with HAdV-3 infection. There have been few case reports of monozygotic twins who developed KD simultaneously, and only two of these reports identified the trigger of KD (Table 2) [510].
Table 2
Previous reports of Kawasaki disease in monozygotic twins
Author (country)
Age (month)
Sex
Interval of onset
Twin A
Twin B
Trigger
(Symptoms of KD/CAAa)
Fink HW.
1985
(USA)
10
female
same day
6/−
6/−
unknown
Hoshino K, et al.
1990
(Japan)
16
female
same day
5/+
5/−
unknown
Kuijpers TW, et al.
2000
(Netherlands)
29
female
3 days
6/−a
5/−
measles virus infection
Ide T, et al.
2007
(Japan)
13
female
1 day
6/−
4/−
measles-rubella vaccine
Kottek A, et al.
2011
(USA)
18
male
2 days
5/−
3/−
unknown
Zhang X, et al.
2013
(China)
2
female
same day
4 / +
4/−
unknown
CAA coronary artery abnormalities, a transient dilation
It has recently been presumed that the etiology of KD combines genetic susceptibility and specific infection, both of which are essential for KD development. Patients with KD seem to have some genetic predisposition, and ethnic differences in the morbidity and familial aggregation of KD have been reported [11]. Some functional single-nucleotide polymorphisms (SNPs) of genes such as inositol 1,4,5-trisphosphate 3-kinase C (ITPKC) and caspase 3 (CAPS3) significantly increase susceptibility to KD [2, 12, 13].
HAdV, group A streptococcus, Staphylococcus, Bacillus cereus, and Yersinia have all been reported as triggering pathogens of KD. In general, KD most commonly develops in infants, toddlers, and young children; adult patients are rarely reported [14]. Most children get infected with common pathogens, such as HAdV, during early childhood, so if these pathogens can trigger KD, this may partially explain the higher KD prevalence in children than that in adults.
HAdV infection itself could be one of the differential diagnoses of KD. HAdV infection also shows KD-like symptoms such as conjunctival injection, red cracked lips, and cervical lymphadenopathy. However, skin erythema and swollen red palms and soles followed by desquamation are distinctive features of KD. Additionally, a coronary lesion can allow the definitive diagnosis of KD. In our case, the younger brother’s echocardiography revealed a dilation of the left circumflex artery, so we definitively diagnosed him with KD.
Several previous reports show that HAdV was detected by PCR in samples from the respiratory tract of patients with KD [1517]. However, it is difficult to distinguish between latent and acute HAdV infection by this method. Coincidental isolation of HAdV by PCR may also occur in some patients with KD. In our case, monozygotic twins simultaneously developed KD after acute HAdV-3 infection. HAdV-3 was detected in stool samples from both patients by PCR, and HAdV3 was directly isolated from the younger brother’s stool sample. Additionally, serum neutralizing antibody to HAdV-3 was significantly elevated in both patients 2 weeks after admission, compared with undetectable levels upon admission. This sero-conversion suggests acute infection. Furthermore, the elevated serum IFN-γ and IL-18 levels observed in these patients might also reflect a systemic inflammatory reaction against an acute viral infection. Therefore, we hypothesize that HAdV infection in children with a genetic susceptibility to KD may abnormally stimulate their innate immunity and evoke a cytokine storm leading to the development of KD. As acute infection can trigger KD, KD may have self-limiting and acute-onset features.

Conclusion

Our report contributes further evidence that a specific response to a pathogen such as HAdV, combined with genetic susceptibility, plays an essential role in the development of KD.

Acknowledgements

None

Funding

None of the authors have any sources of funding to declare in relation to this manuscript.

Availability of data and materials

Not applicable

Authors’ contributions

SF wrote the first draft. SF, MF, and HK treated the patients. JA measured the serum cytokine levels. NH and TF performed virological analyses. SI is the overall supervisor of this study. All authors read and approved the final manuscript.

Competing interests

None of the authors have any financial or any non-financial competing interests to declare in relation to this manuscript.

Consent for publication

Consent has been given by the parents of these patients.

Ethics approval and consent to participate

Not applicable

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