Somatic mutation analysis of MYH11in breast and prostate cancer
- Open Access
- 01.12.2008
- Research article
Erschienen in:
Abstract
Background
MYH11 (also known as SMMHC) encodes the smooth-muscle myosin heavy chain and belongs to the family of conventional myosins. The well-characterized biological function of myosins is their ability to use the energy of ATP hydrolysis to move actin filaments and produce muscle force. Recently, myosins have been implicated in a variety of other intra-cellular functions, including cell migration, adhesion, control of cell shape, and membrane traffic [1], as well as in cell-signaling pathways such as interaction with Rho [2] and the pro-apoptotic protein Bmf (BCL2-modifying factor) [3]. In yeast, myosin 5 has been shown to orientate the mitotic spindle making it pivotal in establishment of cell polarity [4]. Furthermore, several recent publications have shown the significance of nuclear actin and myosin I in transcription [1, 5].
Inversion at the MYH11 locus inv(16)(p13q22) is one of the most frequent chromosomal translocations found in acute myeloid leukemia (AML) and accounts for approximately 8% of all AML cases, especially those of the M4Eo subtype [6]. The inversion results in fusion of the first four to five exons of core binding factor β (CBFβ) and the C-terminal tailpiece of the MYH11 gene and formation of an oncogenic chimeric protein [6].
Anzeige
Recently, myh11 was identified as the predisposing gene for the zebrafish recessive lethal meltdown phenotype. The meltdown mutants develop cystic expansions of the posterior intestine and expanded connective tissue reminiscent of human juvenile polyposis and Peutz-Jeghers syndrome polyps. The mutation observed in the germline of these fish is located in a highly conserved area of the gene and it leads to constitutive activation of the ATPase function, resulting in disruption of the smooth muscle adjacent to posterior intestine [7].
We have previously identified MYH11 as a driver gene in human colorectal cancer [8]. We identified a somatic frameshift mutation (c.5798delC) in the MYH11 gene in 55% of colorectal cancers (CRCs) with microsatellite instability. MYH11 mutations were also found in one microsatellite stable CRC (altogether 30 MSS CRCs analyzed) and in the germline of one patient with Peutz-Jeghers syndrome. Functional assays demonstrated that the mutations (R500L, K1044N and c.5798del/insC) identified in MYH11 in colorectal neoplasia resulted in unregulated proteins with actin-activated motor activity, similar to the mutant myh11 underlying the zebrafish meltdown phenotype [7]. We suggested that MYH11 could play a role in tumor formation by disturbing stem cell differentiation process or through effects on cellular energy balance.
The aim of the current work was to investigate the possible role of somatic MYH11 mutations in two other common cancer types, breast and prostate cancer.
Methods
Breast cancer samples
A total of 155 breast cancer DNA samples were available for the study. The samples were derived from patients unselected for a possible family history of cancer and diagnosed with mammary gland adenocarcinoma between 1988 and 1994 at the Oulu University Hospital. The individuals had a mean age at diagnosis of 55 years (range 29–89 years) [9]. Tumor samples were evaluated by a pathologist prior to DNA extraction and contained at minimum 30% of tumor cells. A total of 34 samples (21.9%) had a tumor content < 50% (approximately 40% of tumor cells). On average the tumor cell content was 50–70%, which should enable detection of somatic mutations by sequencing. Patients gave their informed consent, and approval to perform the study was obtained from the Ethical Board of the Northern Ostrobothnia Health Care District (11/2000, 88/2000) and the Finnish Ministry of Social Affairs and Health (Dnr 46/07/98).
Anzeige
Prostate cancer samples
Genome Phi-amplified (GenomiPhi Amplification kit, Amersham, GE Healthcare, UK) unselected prostate tumour samples (n = 71) from the Tampere region were analysed. Of these 71 samples, 30 were prostatectomy specimens from previously untreated patients. 14 hormone-refractory specimens were obtained from transurethral resection. These patients had been treated by androgen withdrawal, on average, for 38 months (range: 15–68 months). In addition, 6 benign prostate hyperplasia specimens, 16 prostate cancer xenografts (LuCaP 23.1, 23.8, 35, 35V, 49, 58, 69, 70, 73, 77, 78, 81, 92.1, 96, 105, 115, kindly provided by Dr. Robert Vessella, University of Washington, Seattle, WA, USA), and 5 prostate cancer cell lines (DU145, LAPC4, LNCaP, PC-3 and 22Rv1) were available. DU145, LNCaP, PC-3 and 22Rv1 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), and LAPC4 was kindly provided by Dr. Charles Sawyers (MSKCC, New York, NY, USA). Prior to DNA extraction the samples were evaluated by a pathologist to confirm sufficient content of malignant tissue. All tumor samples contained >60% of malignant cells. The use of clinical tumor material has been approved by the Ethical Committee of Tampere University Hospital. All patients participating in this study gave informed consent.
PCR and sequencing
MYH11 (NM_002474 and NM_022844) exons 9, 12, 24, 27, 28, 29, 38 and 40 (isoform SM2) were analyzed using PCR and genomic sequencing. The first coding exon was termed exon 1. MYH11 encodes almost 2000 amino acids (42 exons), and targeted mutation analysis was performed focusing only on the regions that were important in colorectal tumor formation. The mutations which were previously functionally examined were located in exons 12 (R500L), 24 (K1044N) and 40 (SM2, c.5798del/insC) [8].
Sequencing primers have been described previously [8]. PCRs were performed in 25 μl volume containing 20 ng of genomic DNA, 1× PCR buffer (Applied Biosystems, AB, Foster City, California), 200 μmol/l each dNTP (Finnzymes, Espoo, Finland), 0.25 μmol/l both primers, and 1.25 units AmpliTaq Gold DNA polymerase. Samples were purified using ExoSAP-IT PCR purification kit (USB Corporation, Cleveland, Ohio), and sequenced using AB 3730 BD3.1 sequencing chemistry and 5.1 sequencing analysis software (Applied Biosystems, Branchburg, NJ). Genomic (un-amplified) DNA was available from the prostate samples for further analyses.
Results
Most of the studied MYH11 exons were successfully analyzed in 80–100% of samples, with a few exceptions. In prostate cancers, exon 9 was successfully analyzed only in 33% of samples due to technical problems, and in breast cancers results from exon 24 sequencing were derived from 73% of cases. As somatic mutations may be missed due to normal tissue contamination, only sequence traces with no background signal were scored successful to enable detection of even small mutation peaks.
Analysis of 155 breast cancer samples for selected MYH11 exons revealed no somatic alterations. A heterozygous missense alteration A1072T was detected in one patient with breast cancer, which to our knowledge has not been reported in previous studies (Table 1). This alteration was detected also in the respective germline sample. The significance of A1072T was not evaluated further and remains unknown, although it is likely a rare polymorphism. A missense change D1066N was detected in two breast cancer samples and in the corresponding normal tissue samples in heterozygosity. This variant was observed also in our previous work in the germline DNA of Finnish patients with colorectal cancer and is presumably a polymorphism [8].
Table 1
Alterations detected in 155 breast carcinomas and 71 prostate carcinomas (50 primary tumors, 16 xenografts).
Alteration | No of samples | Type of alteration (Reference) |
|---|---|---|
D1066N** | 2 Breast ca | Germline polymorphism [8] |
A1072T** | 1 Breast ca | Germline polymorpism (this study) |
A1234T* | 28 Prostate ca | Germline polymorphism (Ensembl) |
61 Breast ca | ||
K1247K** | 1 Prostate ca | Unknown (this study) |
V1289A* | 3 Prostate ca | Germline polymorphism (Ensembl) |
6 Breast ca | ||
L1323L * | 1 Prostate ca | Germline polymorphism (Ensembl) |
10 Breast ca | ||
A1839A** | 7 Breast ca | Germline polymorphism [8] |
1 Prostate ca | ||
c.5798delC | 1 Prostate ca | Candidate somatic mutation |
A heterozygous alteration K1247K was found in one prostate cancer cell line (Table 1). Unfortunately, no corresponding normal tissue was available and this silent change was not studied further. One prostate cancer sample harbored a frameshift mutation c.5798delC (Table 1) in a microsatellite tract of 8 cytosine units, which we have previously shown to occur frequently in MSI CRC and to result in a protein with unregulated actin-activated motor activity. The sample was re-analyzed using genomic (un-amplified) DNA to confirm the result. Somatic origin of the frameshift mutation or microsatellite-status of this prostate specimen could not be determined due to lack of normal tissue sample. However, in a previous comparative genomic hybridization study [10] the sample showed little signs of chromosomal instability, which could be suggestive of MSI. The sample (LuCaP73) harboring the candidate somatic MYH11 frameshift mutation was a xenograft specimen originating from a hormone-refractory prostate cancer. No other somatic changes were detected in prostate cancer samples.
Discussion
Breast and prostate carcinomas are among the most common cancers in Western countries. The efforts aiming to identify cancer-specific somatic mutations in these cancer types may ideally provide clues to understanding the cellular processes underlying tumor development and lead to diagnostic and therapeutic advances.
Myosins have a well-characterized biological function in the use the energy of ATP hydrolysis to move actin filaments and produce muscle force, but are implicated also in a variety of other cellular functions, many of which are relevant for cancer formation [1]. We have previously shown that somatic mutations in MYH11, which result in unregulated proteins with constitutive activity, contribute to CRC formation. Recently, MYH1 encoding skeletal muscle myosin heavy polypeptide 1 and MYH9 encoding non-muscle myosin heavy chain type A, were identified as candidate breast cancer genes in systematic analyses of the breast cancer genome [10‐12]. Here we have extended the analysis of the role of myosins in tumor development and analyzed sizable sets of breast and prostate cancers for somatic mutations in the key regions of the MYH11 gene (altogether 8 out of 42 coding exons, covering approximately 18% of the coding region).
Anzeige
We analyzed breast and prostate cancer samples for those MYH11 regions that harbored somatic mutations in CRCs. Especially exons 12, 24 and 40 (SM2) were of interest, as we have demonstrated by functional analysis that mutations in these areas result in molecules lacking phosphorylation-dependent regulation. Based on current structural models of myosin regulation, most mutations in the MYH11 head or in the rod domain would not be expected to abolish regulation [13]. Therefore it is possible that mutations only in certain regions of MYH11 contribute to tumor formation. The strategy was to analyze the regions with the greatest potential for discovery of mutations in a large set of non-CRC tumors.
In this study, no somatic MYH11 mutations were found in breast cancer samples. In prostate cancers, an ATPase activating candidate somatic MYH11 mutation (c.5798delC) was identified in one xenograft sample only. This mutation may have occurred by chance and therefore more evidence is needed to establish whether MYH11 has a role in prostate cancer development. However, c.5798delC seems to occur infrequently in prostate cancers. The negative results are unlikely to be due to normal tissue contamination, as the tumor cell content was typically >50%. Certain somatic mutations, undetectable by sequencing, may have been missed in this study. Based on this work, we cannot exclude the occurrence of mutations in other regions of MYH11 and further studies are needed. It would be of interest to study smaller sets of breast and prostate tumors for the entire coding region of MYH11. Nevertheless, the study suggests that somatic MYH11 mutations are not frequent in breast and prostate cancer.
Conclusion
In conclusion, somatic mutations in the regions of MYH11 that are important for colorectal tumor formation are not frequent in breast or prostate cancers.
Acknowledgements
We thank Inga-Lill Svedberg for expert technical assistance. The study was supported by grants from the Academy of Finland, the Finnish Cancer Society, the Sigrid Jusélius Foundation, the Association for International Cancer Research, the European Commission, and by a grant to P.A. from Orion-Farmos Research Foundation.
Anzeige
Open Access
This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License (
https://creativecommons.org/licenses/by/2.0
), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
PA participated in study design, performed molecular genetic analyses, analyzed the data and wrote the manuscript.
AK participated in study design and the critical revision of the manuscript.
RW, KW, and TV provided specimens and clinical data and participated in the critical revision of the manuscript.
LAA designed the study and participated in manuscript preparation.
All authors read and approved the final manuscript.
