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Calcified Tissue International

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11.06.2016 | Original Research

Sonic Hedgehog Promotes Cementoblastic Differentiation via Activating the BMP Pathways

verfasst von: Won-Jung Bae, Q-Schick Auh, Hyun-Chang Lim, Gyu-Tae Kim, Hyun-Soo Kim, Eun-Cheol Kim

Erschienen in: Calcified Tissue International | Ausgabe 4/2016

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Abstract

Although sonic hedgehog (SHH), an essential molecule in embryogenesis and organogenesis, stimulates proliferation of human periodontal ligament (PDL) stem cells, the effects of recombinant human SHH (rh-SHH) on osteoblastic differentiation are unclear. To reveal the role of SHH in periodontal regeneration, expression of SHH in mouse periodontal tissues and its effects on the osteoblastic/cementoblastic differentiation in human cementoblasts were investigated. SHH is immunolocalized to differentiating cementoblasts, PDL cells, and osteoblasts of the developing mouse periodontium. Addition of rh-SHH increased cell growth, ALP activity, and mineralization nodule formation, and upregulated mRNA expression of osteoblastic and cementoblastic markers. The osteoblastic/cementoblastic differentiation of rh-SHH was abolished by the SHH inhibitor cyclopamine (Cy) and the BMP antagonist noggin. rh-SHH increased the expression of BMP-2 and -4 mRNA, as well as levels of phosphorylated Akt, ERK, p38, and JNK, and of MAPK and NF-κB activation, which were reversed by noggin, Cy, and BMP-2 siRNA. Collectively, this study is the first to demonstrate that SHH can promote cell growth and cell osteoblastic/cementoblastic differentiation via BMP pathway. Thus, SHH plays important roles in the development of periodontal tissue, and might represent a new therapeutic target for periodontitis and periodontal regeneration.

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Titel: Sonic Hedgehog Promotes Cementoblastic Differentiation via Activating the BMP Pathways
verfasst von: Won-Jung Bae
Q-Schick Auh
Hyun-Chang Lim
Gyu-Tae Kim
Hyun-Soo Kim
Eun-Cheol Kim
Publikationsdatum: 11.06.2016
Verlag: Springer US
Erschienen in: Calcified Tissue International / Ausgabe 4/2016
Print ISSN: 0171-967X
Elektronische ISSN: 1432-0827
DOI: https://doi.org/10.1007/s00223-016-0155-1

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Sonic Hedgehog Promotes Cementoblastic Differentiation via Activating the BMP Pathways

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