Background
Spermatozoa are immotile as they leave the testis and do not have the ability to fertilize an oocyte. To gain the capacity to fertilize, they must undergo a maturation process in the epididymis. This process occurs via interactions between the sperm and proteins secreted by the epididymal epithelium that result in biochemical and physiological changes to the sperm membrane [
1‐
3]. The changes in the sperm membrane include modification or relocalization of pre-existing proteins or the acquisition of new proteins synthesized by the epididymal epithelium.
The mouse epididymis is divided into four distinct regions based on cellular morphology: the initial segment, the caput, the corpus and the cauda [
4]. Each region creates its own microenvironment in which the epithelial cells secrete proteins in a highly regulated and regionalized manner so that spermatozoa encounter luminal proteins in a specific sequence [
5,
6]. This is illustrated by the region-specific expression of epididymal genes that encode several classes of proteins, such as proteases [
7], protease inhibitors [
8,
9], ion transporters [
10] and beta defensins [
10‐
12]. In addition to exhibiting highly regionalized expression within the epididymis, such proteins also exhibit tissue specificity; some are expressed only in the epididymis such as lipocalin 5 [
13], whereas others are expressed predominantly in the epididymis but also in a few other tissues such as cystatin 8 [
8], suggesting that these proteins have a specific role in this organ. Furthermore, there are several epithelial cell types in the ductus epididymis, and several epididymal genes exhibit cell specificity and are only expressed in the principal cells [
14].
Sperm maturation in the epididymis is an androgen-dependent process. In the absence of androgen, sperm maturation is disrupted, and sperm do not become motile [
15]. The androgen-dependency of the epididymis is suggested by the fact that several epididymal-specific genes are androgen-regulated, such as glutathione peroxidase 5 (
Gpx5) [
16], lipocalin 5 (
Lcn5) [
13] and cysteine-rich secretory protein 1 (
Crisp1) [
17]. Some reports have suggested a role for testicular factors in regulating gene expression in the epididymis. The identity of these factors has not been fully established, but some studies have reported the presence of basic fibroblast growth factor (
bFGF) [
18] and sperm-associated factors [
19].
Numerous genes that are putatively involved in sperm maturation have not been fully characterized. Characterizing these genes is critical for understanding the mechanism of sperm maturation at the cellular level. Our previous gene profiling data identified epididymal genes that were affected by gonadectomy [
20]. One of the interesting genes was
Spag11a (sperm-associated antigen 11A), which requires further characterization to understand its function in the epididymis. The present study analyzed the signal peptide and other functional domains of
Spag11a as well as its tissue specificity, expression regulation and protein localization.
Methods
Experimental animals and RNA extraction
Adult male mice (8 weeks old) strain ddY (Deutschland, Denken and Yoken) were used in this study. All the mice were handled in accordance with the Research Ethic Committee, Faculty of Medicine, University of Indonesia. Mice were given pelleted food and water ad libitum in a room with controlled light (12 h of light, 12 h of darkness) and temperature (27 ± 1 C). To analyze tissue distribution, RNA was extracted from various tissues. To determine androgen dependency, 28 mice were divided into 7 groups of 4 mice each. The following groups were analyzed: control (not castrated), 6 h, 1 d, 3 d, 5 d after castration, and 3 d and 5 d after castration with testosterone replacement therapy. Castration was performed by removing both testis from the mice under anaesthesia. Testosterone replacement therapy was performed by injecting testosterone solution (Nebido, Bayer, Germany) intra muscularly at a dose of 0.5 mg/mouse/day (diluted in 0.9% NaCl) starting at the day of castration. Mice were sacrificed from each group, caput epididymides were collected and total RNA was extracted. Efferent duct ligation (EDL) was performed to analyse the influence of testicular factors. Twenty mice were divided into 5 groups of 4 mice each. The following groups were analyzed: control (un-ligated), 6 h, 1 d, 3 d and 5 d after efferent duct ligation. Ligation was carried out bilaterally by tying the efferent duct using a synthetic non-absorbable polypropylene suture 6–0 (Prolene, NJ, USA). Total caput RNA was extracted from each group. The castrations and efferent duct ligations were performed by making an incision in the scrotum under anesthesia (2.5% 2,2,2-Tribromoethanol, Avertin, Sigma, USA) diluted in 0.9% NaCl. To extract RNA, the epididymis and other tissues were snap-frozen in liquid nitrogen and stored at −80°C until RNA could be extracted. The RNA extractions were performed using the High Pure RNA Tissue Kit (Roche, Germany) according to the manufacturer’s instructions.
Isolation of mouse spermatozoa and luminal fluid
To examine the presence of SPAG11A in the spermatozoa and luminal fluid, two mice were sacrificed and the caput epididymides, the cauda and the vas deferens were isolated and put on a watch glass containing 500 μl PBS. The caput and cauda were punctured gently using a small needle (26G x 1/2”) and incubated at 37°C for 1 hour to allow spermatozoa and luminal fluid to flow out from the duct. To isolate spermatozoa and luminal fluid from the vas deferens, while incubating in 500 μl PBS at 37°C, the ducts were gently squeezed using a round tweezers several times. After incubation, spermatozoa and the luminal fluid were separated by centrifugation at 800 × g for 5 min.
Quantitative real-time RT-PCR
Ten nanograms of total RNA (DNase-treated) was utilized in the quantitative real-time reverse transcription PCR analysis of tissue distribution and the dependence on androgen and testicular factors. A KAPA SYBR FAST One-Step qRT-PCR Universal Kit (KAPA Biosystems, CA, USA) was used according to the manufacturer’s instructions. The primers used in this study were Spag11a_F (ACAGAGAGCGAGCCGTAAAA), Spag11a_R (AGGCACACGGTGTTTCTGAT) producing an amplicon of 113 bp. Mouse beta actin gene was used to normalize
Spag11a expression in each sample. Primers for beta actin were beta actin_F (GATCTGGCACCACACCTTCT) and beta actin_R (GGGGTGTTGAAGGTCTCAAA) producing an amplicon of 138 bp. All primers have annealing temperatures of 60–61°C. The following program was used in the real time qRT-PCR analyses: cDNA synthesis 42°C for 10 min, reverse transcriptase inactivation 95°C for 5 min, denaturation 95°C for 15 sec, annealing 60°C for 30 sec, elongation 72°C for 60 sec. The cycle was repeated 34 times, melting ramp 50–90°C rising 0.5°C every step, acquired melting curve, and final elongation 72°C for 5 min. All samples for
Spag11a, beta actin and standard curves were run in triplicate. Normalization values presented in each graph were obtained by dividing amplification product using
Spag11a primers (in nanogram unit) with amplification product from beta actin (
Actb) primers. In every qRT-PCR run, two negative controls were included, non-template negative control and minus RT negative control. Additional files show an example of detail calculation of relative gene expression (See Additional file
1: Figure S1),
Spag11a melting curve with its RT-PCR product run on a 1% agarose gel (See Additional file
2: Figure S2) and reaction efficiency (See Additional file
3: Table S1).
Western blot analyses
Protein samples from four different regions of the epididymis (initial segment, caput, corpus and cauda) and also from spermatozoa isolated from epididymis duct and vas deferens were extracted by solubilizing the tissue or cell in sodium dodecyl sulfate (SDS) extraction buffer [2% SDS, 10% sucrose, 0.1875 M Tris (pH 6.8)] suplemented with a protease inhibitor cocktail (Roche, Manneheim, Germany) for 5 min at 100°C. Soluble protein was obtained by centrifugation at 9000 × g for 10 min. Fifteen microgram of protein was then separated by 10% SDS-PAGE and transferred to Hybond-P PVDF membranes (Amersham, Buckinghamshire, UK). The membranes were blocked in 5% Bovine Serum Albumin in 1x TBST for 1 hour at room temperature. The membranes were then incubated overnight at 4°C with rabbit anti-human SPAG11A polyclonal antibody (Immunogen: ag9846, genebank no: BC058833) (Proteintech, USA) at a 1:1000 dilution. The antibody recognize a protein with MW = 20 kDa. The membranes were washed with 1x TBST for 3 × 5 minutes and incubated with donkey anti-rabbit IgG-conjugated horseradish peroxidase (HRP) (Santa Cruz Biotechnology, CA, USA) at a 1:5000 dilution for 1 hour at room temperature. The membranes were washed again with 1x TBST for 3 × 5 minutes, and HRP was detected using a western blot chemiluminescence detection system (Amersham, Buckinghamshire, UK). Chemiluminescence was exposed on x-ray negative film (Fuji Co, Japan).
Immunohistochemistry
Epididymal tissue sections (5 μm thick) and sperm cells were attached to the poly-L-lysine-coated slide and used in immunohisto- and cytochemical analyses. After deparaffinization and rehydration, the sections were expose to 3% hydrogen peroxide in distilled water for 10 min. Antigen retrieval was performed by boiling the slide in 10 mM Na Citrate, pH 6,0, for 3 × 5 min and cooling it slowly to room temperature. Immunostaining was performed using the TrekAvidin-HRP Label kit (Biocare Medical, CA, USA) according to the manufacturer’s instructions. Tissues or cells were incubated overnight with a rabbit anti-human SPAG11A polyclonal antibody at a 1:200 dilution in TBS. All the incubations were performed in a humidified chamber. Color development was achieved by incubating the tissues or cells with DAB (Biocare Medical, CA, USA) and was terminated by incubating the slides in distilled water. The slides were then dehydrated in increasing concentrations of ethanol and a series of xylene solutions before being mounted with Entellan mounting medium (Merck, Darmstadt, Germany) and cover-slipped. For the immunocytochemical analyses, deparafinization, rehydration, antigen retrieval and dehydration were skipped.
Statistical analyses
SigmaStat for Windows Version 3.10 was used to perform a one-way analysis of variance for real time qRT-PCR of the dependence on androgen and testicular factors. The difference between the means was subsequently assessed using the Holm-Sidak test. The level of significance was set at P < 0.05
Discussion
Spermatozoa are transcriptionally and translationally silent cells. The development from immature to mature cells capable of fertilizing an oocyte depends on post-translational modification of pre-existing proteins. These modifications occur via interactions with proteins secreted by the epididymal epithelium while sperm traverse the epididymis. We characterized Spag11a in the mouse epididymis at both the RNA and protein levels to obtain data on the putative role of this gene in sperm maturation.
SPAG11A is a member of the beta defensin family and contains a signal peptide sequence. This protein family has antimicrobial activity and is involved in host defense [
25]. The defensin gene family has evolved by repeated gene duplication and divergence, including functional diversification [
26]. Reproductive functions are suggested by the surface localization of several defensins, including SPAG11 [
27], DEFB118 [
28] and DEFB126 [
29], on sperm. SPAG11E, also known as
Bin1b, is known to promote motility in immature spermatozoa in the caput epididymis via a calcium uptake-dependent mechanism [
30]. Alternative spliced transcript have produced multiple
Spag11 isoforms in epididymal epithelial cells. Although many members of the beta defensin family have been characterized, the exact role of
Spag11a in the mouse epididymis is unknown. The presence of a signal peptide sequence suggested that mouse SPAG11A is a secretory protein that may be involved in sperm maturation. Moreover, we discovered that SPAG11A contains phosphorylation sites such that upon binding to sperm, the cell can be modified by protein kinases. This corresponded with a finding that during transit through the epididymis, spermatozoa undergo changes in tyrosine phosphorylation [
31]. Concerning binding to sperm, we identified a myristoylation site in SPAG11A. The presence of this potential N-myristoylation site suggested that the protein may covalently bind to the plasma membrane of sperm [
32,
33].
Our data demonstrated that mouse
Spag11a was expressed exclusively in the epididymis, not in other tissues such as the testis, vas deferens, intestine, kidney, liver, heart, muscle and brain. Moreover, mouse
Spag11a exhibited a region-specific expression pattern and was mainly present in the caput region. This is similar to several genes that are important for sperm maturation such as
Rnase10[
34,
35],
Crisp4[
36,
37], and
Crisp1[
17], which are exclusively expressed in the initial segment, caput and corpus/cauda, respectively. This region-specific expression is important to create specific environments for sperm maturation. Although the highest expression was detected in the caput, low expression levels were detected in corpus epididymis, muscle and liver (Figure
2). This is possible because the beta defensin family has diverse members, and some of which function in the muscle [
38]. A study by Yamaguchi
et al. also showed multiple epididymis-specific beta defensin isoforms in human and mice, including mouse EP2e, which is a synonym of
Spag11a, with expression in caput, corpus and cauda [
39]. Our study is first to show
Spag11a expression specificity in the caput region both at the transcript and protein levels.
Sperm maturation in the epididymis is androgen-dependent. Our data demonstrated that
Spag11a was slightly up-regulated 6 hours to 1 day after castration/gonadectomy before being dramatically down-regulated 3 days after castration. The androgen dependence was confirmed with a rescue experiment in which exogenous testosterone was injected daily into castrated mice. The results indicated that exogenous testosterone could maintain
Spag11a expression at nearly normal levels on days 3 and 5 after castration. This indicated that
Spag11a was primarily regulated by circulating androgen. Moreover, our study in the regulation of
Spag11a by androgen was also confirmed by immunohistochemistry which demonstrated a total lost of staining in the caput principal cells after 3 d castration. This is similar to the regulation of several genes involved in sperm maturation, such as
Eppin (epididymal protease inhibitor) [
40],
Pate (cysteine rich prostate and testis expressed protein) [
41] and the serine protease inhibitor
HongrES1[
42]. EPPIN is an antimicrobial cysteine-rich protein that contains both Kunitz and whey acidic protein (WAP) domains and is a target for male contraception because of its critical role in sperm motility [
43].
In addition to androgen, epididymal genes are also regulated by testicular factors. Because the observed recovery of
Spag11a expression after gonadectomy/castration with testosterone replacement was slightly lower than that in the control non-gonadectomy group, we tested the possibility that testicular factors were involved in
Spag11a regulation. By using efferent duct ligation (EDL), we blocked testicular fluid (lumicrine factor) from entering the epididymis while preserving testosterone supply from both testis. The result from the EDL experiment showed that the lack of testicular fluid did not affect
Spag11a expression at 6 hours to 1 day after the ligation. Interestingly,
Spag11a was transiently up-regulated at 3 days after EDL before down-regulated back to the level of control at 5 days after EDL (Figure
4). The reason for the transient increase is not known, but we hypothesize that it may be caused by initiation or the onset of apoptosis within the cell which somehow stimulates a temporary up-regulation of
Spag11a in response to the process. This notion is based on previous studies that orchidectomy and efferent duct ligation induces apoptotic cell death in the caput epididymis that reach maximum at day 3 [
44,
45]. Several epididymal genes are regulated by testicular factors, including gamma-glutamyltransferase 1 (
Ggt1, regulated by fibroblast growth factor (FGF)) [
46], 5-alpha reductase (regulated by androgen binding protein (ABP)) [
47] and proenkephalin (
Penk, regulated by sperm-associated factors) [
19]. The primary regulation of caput-specific
Spag11a by androgen confirmed our previous report that epididymal genes enriched in the initial segment are more dependent on testicular factors whereas androgen regulates most of the caput-enriched genes [
20].
We also analyzed whether the expression of
Spag11a mRNA was consistent with the protein expression. We performed western immunoblotting using protein extracts from four different regions of the mouse epididymis. SPAG11A protein was detected with a rabbit anti-human SPAG11A polyclonal antibody. The results demonstrated that SPAG11A was only present in the caput region (Figure
5) which confirmed the antibody specificity. Because the protein was present in the caput epididymis, we performed immunohistochemistry to localize SPAG11A at the subcellular level. SPAG11A was localized in the nucleus and cytoplasm of the principal cells in the caput region, whereas only cytoplasmic staining was detected in the corpus and cauda. This is in agreement with the tissue distribution analyses using qRT-PCR in which the highest expression was in caput whereas very weak expression was detected in the corpus and cauda (Figure
2). The cell-specific expression of SPAG11A confirmed its putative role as a secretory protein that creates a microenvironment suitable for sperm maturation. Principal cells contain secretory apparatuses, such as endoplasmic reticulum, Golgi and secretory granules, and endocytic apparatuses, including coated pits, endosomes, multivesicular bodies and lysosomes. Therefore, the primary functions of principal cells are to synthesize and secrete proteins and to perform endocytosis [
4]. This is particularly interesting because a signal peptide sequence was identified in the first twenty amino acids of the N-terminus of SPAG11A (Figure
1), which is characteristic of secretory proteins. An epididymal gene known to have a cell type-specific expression pattern is cystic fibrosis transmembrane conductance regulator (CFTR) which is also expressed specifically in the principal cell to release ATP into epididymal lumen [
48].
It is intriguing to observe that SPAG11A, with a signal peptide indicating a secretory protein was localized in the nucleus. Although this is an unusual phenomenon, examples of similar protein behaviour do exist. A study of ADAMTS13, a secreted zinc metalloprotease involved in an array of processes including development and angiogenesis, detected the protein in the nucleus of liver cells [
49]. Other metalloproteinases, MMP-2 [
50] and MMP3 [
51], which are involved in extracellular matrix remodeling, were detected in the nucleus of cardiac myocytes and chondrocytic cells, respectively. This suggests intracellular role for these secreted proteins. MMP3, for instance, behaves as a proteinase that degrades matrix components following its secretion, while behaving as a transcription factor when present within the nucleus. It is also possible that SPAG11A has another intracellular role and it shuttles between nucleus and cytoplasm through nuclear pore complex. This is particularly interesting if we correlate it with transient up-regulation of
Spag11a at 3 d following efferent duct ligation (EDL) and returned to the normal level at 5 d post EDL. That coincided with a major change in the caput epididymal cell at day 3 following orchidectomy or EDL, particularly the onset of apoptosis [
44,
45].
Our study is the first to show that SPAG11A is a secretory protein that is present in the epididymal fluid and spermatozoa taken from the cauda epididymis and vas deferens. SPAG11A is secreted mainly by principal cells of the caput epididymis and the protein was detected in epididymal fluid but minimum amount of protein was detected in the vas deferens fluid. The reduced amount of protein in the vas deferens luminal fluid indicating that most of the protein may have bound to the sperm cell. Secreted from the caput and to some extent from corpus and cauda region, the protein subsequently bind to the spermatozoa. An increasing amount of SPAG11A was detected in the protein extracted from cauda and vas deferens sperm compared to the caput sperm (Figure
8A). Furthermore, by using immunocytochemistry, we also showed more intense SPAG11A staining in the sperm cells taken from vas deferens compared to the epididymal sperm, confirming more SPAG11A protein deposited to the sperm upon exit from the epididymis. We believe that data from this study is important for a further study to determine the role of SPAG11A during epididymal sperm maturation and fertilization.
Competing interests
The authors declare that they have no competing interests.
Authors’ contributions
DAP contributed in designing and performing the gene expression analyses, androgen dependency experiments and immunocytochemistry and by drafting the manuscript. EL contributed to the gene expression analyses, androgen dependency experiments, protein analyses and immunocytochemistry. PS and YHM participated in designing the experiments and interpreting the data. PSH contributed to designing the experiments, interpreting the data and revising the manuscript. All authors read and approved the final manuscript.