nach oben

Journal of Assisted Reproduction and Genetics

Erschienen in:

17.06.2022 | Review

Sperm cryopreservation and DNA methylation: possible implications for ART success and the health of offspring

verfasst von: Zahra Khosravizadeh, Kajal Khodamoradi, Zahra Rashidi, Malihe Jahromi, Elham Shiri, Ensieh Salehi, Ali Talebi

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 8/2022

Einloggen, um Zugang zu erhalten

Abstract

Despite the beneficial effects of sperm cryopreservation, increased reactive oxygen species (ROS) production during this process can affect spermatozoon structure and function. Moreover, ROS production is associated with elevated DNA damage and alterations in DNA methylation. There is little information about the effects of cryopreservation on epigenetic modulation in sperm and the health of children born with frozen spermatozoa. Considering the potential consequences of cryopreservation in ART-conceived children, it is necessary to assure that cryopreservation does not modify sperm DNA methylation status. This review summarizes reports on epigenetic modifications of spermatozoa during cryopreservation and the probable effects of this process on offspring health. Contradictory results have reported the influence of sperm cryopreservation on DNA methylation in imprinted genes. Multiclinical studies with larger sample sizes under the same conditions of cryopreservation and DNA methylation analysis are needed to make any definitive conclusion about the effect of the cryopreservation process on sperm DNA methylation.

Di Santo M, Tarozzi N, Nadalini M, Borini A. Human sperm cryopreservation: update on techniques, effect on DNA integrity, and implications for ART. Ther Adv Urol. 2011;2012:854837. https://doi.org/10.1155/2012/854837.CrossRef

Medeiros C, Forell F, Oliveira A, Rodrigues J. Current status of sperm cryopreservation: why isn’t it better? Theriogenology. 2002;57(1):327–44. https://doi.org/10.1016/S0093-691X(01)00674-4.CrossRefPubMed

Hezavehei M, Sharafi M, Kouchesfahani HM, Henkel R, Agarwal A, Esmaeili V, et al. Sperm cryopreservation: a review on current molecular cryobiology and advanced approaches. Reprod BioMed Online. 2018;37(3):327–39. https://doi.org/10.1016/j.rbmo.2018.05.012.CrossRefPubMed

Valipour J, Nashtaei MS, Khosravizadeh Z, Mahdavinezhad F, Nekoonam S, Esfandyari S, et al. Effect of sulforaphane on apoptosis, reactive oxygen species and lipids peroxidation of human sperm during cryopreservation. Cryobiology. 2020. https://doi.org/10.1016/j.cryobiol.2020.11.012.

Barlow DP, Bartolomei MS. Genomic imprinting in mammals. Cold Spring Harb Perspect Biol. 2014;6(2):a018382. https://doi.org/10.1101/cshperspect.a018382.CrossRefPubMedPubMedCentral

Millership SJ, Van de Pette M, Withers DJ. Genomic imprinting and its effects on postnatal growth and adult metabolism. Cell Mol Life Sci. 2019;76(20):4009–21. https://doi.org/10.1007/s00018-019-03197-z.CrossRefPubMedPubMedCentral

Amor DJ, Halliday J. A review of known imprinting syndromes and their association with assisted reproduction technologies. Hum Reprod. 2008;23(12):2826–34. https://doi.org/10.1093/humrep/den310.CrossRefPubMed

Kläver R, Bleiziffer A, Redmann K, Mallidis C, Kliesch S, Gromoll J. Routine cryopreservation of spermatozoa is safe—evidence from the DNA methylation pattern of nine spermatozoa genes. J Assist Reprod Genet. 2012;29(9):943–50. https://doi.org/10.1007/s10815-012-9813-z.CrossRefPubMedPubMedCentral

Lu W-H, Yang X-Y, Liang X-W, Gu Y-Q. AB082. Effect of cryopreservation on DNA methylation status of imprinted genes in human sperm. Transl Androl Urol. 2015;4(Suppl 1):402–8. https://doi.org/10.3978/j.issn.2223-4683.2015.s082.CrossRef

10.

Coll-Sanmartin, Laia; Vidal, Francesca, dir. Effect of cryopreservation in the imprinting pattern of gametes. 2015. 1 pag. (833 Grau en Genètica). https://ddd.uab.cat/record/141371

11.

Juan CA, Pérez de la Lastra JM, Plou FJ, Pérez-Lebeña E. The chemistry of reactive oxygen species (ROS) revisited: outlining their role in biological macromolecules (DNA, lipids and proteins) and induced pathologies. Int J Mol Sci. 2021;22(9):4642. https://doi.org/10.3390/ijms22094642.CrossRefPubMedPubMedCentral

12.

Gualtieri R, Kalthur G, Barbato V, Longobardi S, Di Rella F, Adiga SK, et al. Sperm oxidative stress during in vitro manipulation and its effects on sperm function and embryo development. Antioxidants. 2021;10(7):1025. https://doi.org/10.3390/antiox10071025.CrossRefPubMedPubMedCentral

13.

Shiri E, Abolhassani F, Khosravizadeh Z, Najafi A, Khanezad M, Vazirian M, et al. Aqueous Origanum vulgare extract improves the quality of cryopreserved human spermatozoa through its antioxidant effects. Biopreserv Biobank. 2020;18(4):329–36. https://doi.org/10.1089/bio.2020.0008.CrossRefPubMed

14.

Lasso JL, Noiles EE, Alvarez JG, Storey BT. Mechanism of superoxide dismutase loss from human sperm cells during cryopreservation. J Androl. 1994;15(3):255–65. https://doi.org/10.1002/j.1939-4640.1994.tb00444.x.CrossRefPubMed

15.

Ozkavukcu S, Erdemli E, Isik A, Oztuna D, Karahuseyinoglu S. Effects of cryopreservation on sperm parameters and ultrastructural morphology of human spermatozoa. J Assist Reprod Genet. 2008;25(8):403–11. https://doi.org/10.1007/s10815-008-9232-3.CrossRefPubMedPubMedCentral

16.

Said TM, Gaglani A, Agarwal A. Implication of apoptosis in sperm cryoinjury. Reprod BioMed Online. 2010;21(4):456–62. https://doi.org/10.1016/j.rbmo.2010.05.011.CrossRefPubMed

17.

Koppers AJ, Mitchell LA, Wang P, Lin M, Aitken RJ. Phosphoinositide 3-kinase signalling pathway involvement in a truncated apoptotic cascade associated with motility loss and oxidative DNA damage in human spermatozoa. Biochem J. 2011;436(3):687–98. https://doi.org/10.1042/BJ20110114.CrossRefPubMed

18.

Murphy MP. How mitochondria produce reactive oxygen species. Biochem J. 2009;417(1):1–13. https://doi.org/10.1042/BJ20081386.CrossRefPubMed

19.

Aitken RJ, Drevet JR. The importance of oxidative stress in determining the functionality of mammalian spermatozoa: a two-edged sword. Antioxidants. 2020;9(2):111. https://doi.org/10.3390/antiox9020111.CrossRefPubMedCentral

20.

O’connell M, Mcclure N, Lewis S. The effects of cryopreservation on sperm morphology, motility and mitochondrial function. Hum Reprod. 2002;17(3):704–9. https://doi.org/10.1093/humrep/17.3.704.CrossRefPubMed

21.

Fortunato A, Leo R, Liguori F. Effects of cryostorage on human sperm chromatin integrity. Zygote. 2013;21(4):330–6.CrossRef

22.

Kalthur G, Adiga SK, Upadhya D, Rao S, Kumar P. Effect of cryopreservation on sperm DNA integrity in patients with teratospermia. Fertil Steril. 2008;89(6):1723–7. https://doi.org/10.1016/j.fertnstert.2007.06.087.CrossRefPubMed

23.

Lusignan M-F, Li X, Herrero B, Delbès G, Chan PT. Effects of different cryopreservation methods on DNA integrity and sperm chromatin quality in men. Andrology. 2018;6(6):829–35. https://doi.org/10.1111/andr.12529.CrossRefPubMed

24.

Chohan K, Griffin J, Carrell D. Evaluation of chromatin integrity in human sperm using acridine orange staining with different fixatives and after cryopreservation. Andrologia. 2004;36(5):321–6. https://doi.org/10.1111/j.1439-0272.2004.00626.x.CrossRefPubMed

25.

Hosseini A, Khalili MA, Talebi AR, Agha-Rahimi A, Ghasemi-Esmailabad S, Woodward B, et al. Cryopreservation of low number of human spermatozoa; which is better: vapor phase or direct submerging in liquid nitrogen? Hum Fertil. 2018. https://doi.org/10.1080/14647273.2018.1456681.

26.

Isachenko E, Isachenko V, Katkov II, Rahimi G, Schondorf T, Mallmann P, et al. DNA integrity and motility of human spermatozoa after standard slow freezing versus cryoprotectant-free vitrification. Hum Reprod. 2004;19(4):932–9. https://doi.org/10.1093/humrep/deh194.CrossRefPubMed

27.

Morshedi M, Duru N, Oehninger S. Cryopreservation-thawing does not impair DNA integrity but induces plasma membrane translocation of phosphatidylserine in fractionated human spermatozoa. Fertil Steril. 2000;74(3):S244–S5. https://doi.org/10.1016/S0015-0282(00)01445-X.CrossRef

28.

Bogle O, Kumar K, Attardo-Parrinello C, Lewis S, Estanyol J, Ballescà J, et al. Identification of protein changes in human spermatozoa throughout the cryopreservation process. Andrology. 2017;5(1):10–22. https://doi.org/10.1111/andr.12279.CrossRefPubMed

29.

Paoli D, Lombardo F, Lenzi A, Gandini L. Sperm cryopreservation: effects on chromatin structure. Gen Damage Human Spermatozoa. 2014;2014:137–50. https://doi.org/10.1007/978-1-4614-7783-9_9.CrossRef

30.

Lister R, Pelizzola M, Dowen RH, Hawkins RD, Hon G, Tonti-Filippini J, et al. Human DNA methylomes at base resolution show widespread epigenomic differences. Nature. 2009;462(7271):315–22. https://doi.org/10.1038/nature08514.CrossRefPubMedPubMedCentral

31.

Lee J-H, Park S-J, Nakai K. Differential landscape of non-CpG methylation in embryonic stem cells and neurons caused by DNMT3s. Sci Rep. 2017;7(1):1–11. https://doi.org/10.1038/s41598-017-11800-1.CrossRef

32.

Bird A, Taggart M, Frommer M, Miller OJ, Macleod D. A fraction of the mouse genome that is derived from islands of nonmethylated. CpG-rich DNA Cell. 1985;40(1):91–9. https://doi.org/10.1016/0092-8674(85)90312-5.CrossRefPubMed

33.

Wu Q, Ni X. ROS-mediated DNA methylation pattern alterations in carcinogenesis. Curr Drug Targets. 2015;16(1):13–9. https://doi.org/10.2174/1389450116666150113121054.CrossRefPubMed

34.

Campos AC, Molognoni F, Melo FH, Galdieri LC, Carneiro CR, D’Almeida V, et al. Oxidative stress modulates DNA methylation during melanocyte anchorage blockade associated with malignant transformation. Neoplasia. 2007;9(12):1111–21. https://doi.org/10.1593/neo.07712.CrossRefPubMedPubMedCentral

35.

Ziech D, Franco R, Pappa A, Panayiotidis MI. Reactive oxygen species (ROS)––induced genetic and epigenetic alterations in human carcinogenesis. Mutat Res-Fund Mol Mutagen. 2011;711(1-2):167–73. https://doi.org/10.1016/j.mrfmmm.2011.02.015.CrossRef

36.

Valinluck V, Sowers LC. Endogenous cytosine damage products alter the site selectivity of human DNA maintenance methyltransferase DNMT1. Cancer Res. 2007;67(3):946–50. https://doi.org/10.1158/0008-5472.CAN-06-3123.CrossRefPubMed

37.

Shao D, Fry JL, Han J, Hou X, Pimentel DR, Matsui R, et al. A redox-resistant sirtuin-1 mutant protects against hepatic metabolic and oxidant stress. J Biol Chem. 2014;289(11):7293–306. https://doi.org/10.1074/jbc.M113.520403.CrossRefPubMedPubMedCentral

38.

El-Osta A, Wolffe AP. DNA methylation and histone deacetylation in the control of gene expression: basic biochemistry to human development and disease. Gene Expr J Liver Res. 2001;9(1-2):63–75. https://doi.org/10.3727/000000001783992731.CrossRef

39.

Song C, Feodorova Y, Guy J, Peichl L, Jost KL, Kimura H, et al. DNA methylation reader MECP2: cell type- and differentiation stage-specific protein distribution. Epigenetics Chromatin. 2014;7(1):1–16. https://doi.org/10.1186/1756-8935-7-17.CrossRef

40.

Weng S-M, Bailey ME, Cobb SR. Rett syndrome: from bed to bench. Pediatr Neonatol. 2011;52(6):309–16. https://doi.org/10.1016/j.pedneo.2011.08.002.CrossRefPubMed

41.

Petrussa L, Van de Velde H, De Rycke M. Dynamic regulation of DNA methyltransferases in human oocytes and preimplantation embryos after assisted reproductive technologies. Mol Hum Reprod. 2014;20(9):861–74. https://doi.org/10.1093/molehr/gau049.CrossRefPubMed

42.

Zeng C, Peng W, Ding L, He L, Zhang Y, Fang D, et al. A preliminary study on epigenetic changes during boar spermatozoa cryopreservation. Cryobiology. 2014;69(1):119–27. https://doi.org/10.1016/j.cryobiol.2014.06.003.CrossRefPubMed

43.

Oblette A, Rondeaux J, Dumont L, Delessard M, Saulnier J, Rives A, et al. DNA methylation and histone post-translational modifications in the mouse germline following in-vitro maturation of fresh or cryopreserved prepubertal testicular tissue. Reprod BioMed Online. 2019;39(3):383–401. https://doi.org/10.1016/j.rbmo.2019.05.007.CrossRefPubMed

44.

Hu Y, Li L, Yu Y, Huang H, Uygun BE, Yarmush ML. RNA-based dCas9–VP64 system improves the viability of cryopreserved mammalian cells. Nano Life. 2018;8(03):1850004. https://doi.org/10.1142/S1793984418500046.CrossRef

45.

Yan L-Y, Yan J, Qiao J, Zhao P-L, Liu P. Effects of oocyte vitrification on histone modifications. Reprod Fertil Dev. 2010;22(6):920–5. https://doi.org/10.1071/RD09312.CrossRefPubMed

46.

Maldonado MBC, Penteado JCT, Faccio BMC, Lopes FL, Arnold DR. Changes in tri-methylation profile of lysines 4 and 27 of histone H3 in bovine blastocysts after cryopreservation. Cryobiology. 2015;71(3):481–5. https://doi.org/10.1016/j.cryobiol.2015.09.001.CrossRefPubMed

47.

Aurich C, Schreiner B, Ille N, Alvarenga M, Scarlet D. Cytosine methylation of sperm DNA in horse semen after cryopreservation. Theriogenology. 2016;86(5):1347–52. https://doi.org/10.1016/j.theriogenology.2016.04.077.CrossRefPubMed

48.

Zhou XWYTL, Ma L, Yan B, Tian J, Zhang F, Zhou Y, Wang H. DNA methylation and expression of SNRPN and GRB10 imprinted genes in human semen freezing-thawing process. J Shandong Univ. 2017;152(2–3):373–80. https://doi.org/10.6040/j.issn.1671-7554.0.2016.1065.CrossRef

49.

de Mello F, Garcia JS, Godoy LC, Depincé A, Labbé C, Streit DP Jr. The effect of cryoprotectant agents on DNA methylation patterns and progeny development in the spermatozoa of Colossoma macropomum. Gen Comp Endocrinol. 2017;245:94–101. https://doi.org/10.1016/j.ygcen.2016.06.003.CrossRefPubMed

50.

Depincé A, Gabory A, Dziewulska K, Le Bail PY, Jammes H, Labbé C. DNA methylation stability in fish spermatozoa upon external constraint: Impact of fish hormonal stimulation and sperm cryopreservation. Mol Reprod Dev. 2020;87(1):124–34. https://doi.org/10.1002/mrd.23297.CrossRefPubMed

51.

Khosravizadeh Z, Hassanzadeh G, Bazzaz JT, Alizadeh F, Totonchi M, Salehi E, et al. The effect of cryopreservation on DNA methylation patterns of the chromosome 15q11–q13 region in human spermatozoa. Cell Tissue Bank. 2020;21(3):433–45. https://doi.org/10.1007/s10561-020-09828-1.CrossRefPubMed

52.

Mercati F, Domingo P, Pasquariello R, Dall’Aglio C, Di Michele A, Forti K, et al. Effect of chelating and antioxidant agents on morphology and DNA methylation in freeze-drying rabbit (Oryctolagus cuniculus) spermatozoa. Reprod Domest Anim. 2020;55(1):29–37. https://doi.org/10.1111/rda.13577.CrossRefPubMed

53.

Salehi M, Mahdavi AH, Sharafi M, Shahverdi A. Cryopreservation of rooster semen: evidence for the epigenetic modifications of thawed sperm. Theriogenology. 2020;142:15–25. https://doi.org/10.1016/j.theriogenology.2019.09.030.CrossRefPubMed

54.

Peris-Frau P, Álvarez-Rodríguez M, Martín-Maestro A, Iniesta-Cuerda M, Sánchez-Ajofrín I, Medina-Chávez DA, et al. Unravelling how in vitro capacitation alters ram sperm chromatin before and after cryopreservation. Andrology. 2021;9(1):414–25. https://doi.org/10.1111/andr.12900.CrossRefPubMed

55.

Güngör İH, Tektemur A, Arkali G, Cinkara SD, Acisu TC, Koca RH, et al. Effect of freeze–thawing process on lipid peroxidation, miRNAs, ion channels, apoptosis and global DNA methylation in ram spermatozoa. Reprod Fertil Dev. 2021;33(14):747–59. https://doi.org/10.1071/RD21091.CrossRefPubMed

56.

Montjean D, Zini A, Ravel C, Belloc S, Dalleac A, Copin H, et al. Sperm global DNA methylation level: association with semen parameters and genome integrity. Andrology. 2015;3(2):235–40. https://doi.org/10.1111/andr.12001.CrossRefPubMed

57.

Gopalkrishnan K, Hinduja I, Anand KT. In vitro decondensation of nuclear chromatin of human spermatozoa: assessing fertilizing potential. Arch Androl. 1991;27(1):43–50. https://doi.org/10.3109/01485019108987650.CrossRefPubMed

58.

Peris-Frau P, Martín-Maestro A, Iniesta-Cuerda M, Sánchez-Ajofrín I, Cesari A, Garde JJ, et al. Cryopreservation of ram sperm alters the dynamic changes associated with in vitro capacitation. Theriogenology. 2020;145:100–8. https://doi.org/10.1016/j.theriogenology.2020.01.046.CrossRefPubMed

59.

Raz E. The function and regulation of vasa-like genes in germ-cell development. Genome Biol. 2000;1(3):1–6. https://doi.org/10.1186/gb-2000-1-3-reviews1017.CrossRef

60.

Stürchler D, Tanner M, Hanck A, Betschart B, Gautschi K, Weiss N, et al. A longitudinal study on relations of retinol with parasitic infections and the immune response in children of Kikwawila village, Tanzania. Acta Trop. 1987;44(2):213–27.PubMed

61.

Riesco MF, Robles V. Cryopreservation causes genetic and epigenetic changes in zebrafish genital ridges. PLoS One. 2013;8(6):e67614. https://doi.org/10.1371/annotation/3265139d-64c7-4c4c-83d3-1e139031e7df.CrossRefPubMedPubMedCentral

62.

Al Ageeli E, Drunat S, Delanoë C, Perrin L, Baumann C, Capri Y, et al. Duplication of the 15q11-q13 region: clinical and genetic study of 30 new cases. Eur J Med Genet. 2014;57(1):5–14. https://doi.org/10.1016/j.ejmg.2013.10.008.CrossRefPubMed

63.

Valcarce D, Cartón-García F, Riesco M, Herráez M, Robles V. Analysis of DNA damage after human sperm cryopreservation in genes crucial for fertilization and early embryo development. Andrology. 2013;1(5):723–30. https://doi.org/10.1111/j.2047-2927.2013.00116.x.CrossRefPubMed

64.

Yoshimizu T, Miroglio A, Ripoche M-A, Gabory A, Vernucci M, Riccio A, et al. The H19 locus acts in vivo as a tumor suppressor. Proc Natl Acad Sci. 2008;105(34):12417–22. https://doi.org/10.1073/pnas.0801540105.CrossRefPubMedPubMedCentral

65.

Kläver R, Tüttelmann F, Bleiziffer A, Haaf T, Kliesch S, Gromoll J. DNA methylation in spermatozoa as a prospective marker in andrology. Andrology. 2013;1(5):731–40. https://doi.org/10.1111/j.2047-2927.2013.00118.x.CrossRefPubMed

66.

Thomson LK, Fleming SD, Aitken RJ, De Iuliis GN, Zieschang J-A, Clark AM. Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis. Hum Reprod. 2009;24(9):2061–70. https://doi.org/10.1093/humrep/dep214.CrossRefPubMed

67.

Donnelly ET, McClure N, Lewis SE. Cryopreservation of human semen and prepared sperm: effects on motility parameters and DNA integrity. Fertil Steril. 2001;76(5):892–900. https://doi.org/10.1016/S0015-0282(01)02834-5.CrossRefPubMed

68.

Tunc O, Tremellen K. Oxidative DNA damage impairs global sperm DNA methylation in infertile men. J Assist Reprod Genet. 2009;26(9):537–44. https://doi.org/10.1007/s10815-009-9346-2.CrossRefPubMedPubMedCentral

69.

Olszewska M, Barciszewska MZ, Fraczek M, Huleyuk N, Chernykh VB, Zastavna D, et al. Global methylation status of sperm DNA in carriers of chromosome structural aberrations. Asian J Androl. 2017;19(1):117. https://doi.org/10.4103/1008-682X.168684.CrossRefPubMed

70.

Hiura H, Okae H, Chiba H, Miyauchi N, Sato F, Sato A, et al. Imprinting methylation errors in ART. Reprod Med Biol. 2014;13(4):193–202. https://doi.org/10.1007/s12522-014-0183-3.CrossRefPubMedPubMedCentral

71.

Khezri A, Narud B, Stenseth E-B, Johannisson A, Myromslien FD, Gaustad AH, et al. DNA methylation patterns vary in boar sperm cells with different levels of DNA fragmentation. BMC Genomics. 2019;20(1):1–15. https://doi.org/10.1186/s12864-019-6307-8.CrossRef

72.

Nusbaumer D, Marques da Cunha L, Wedekind C. Sperm cryopreservation reduces offspring growth. Proc R Soc B. 2019;286(1911):20191644. https://doi.org/10.1098/rspb.2019.1644.CrossRefPubMedPubMedCentral

73.

Cai J, Liu L, Chen J, Liu Z, Wang W, Jiang X, et al. The effect of epididymal sperm cryopreservation on neonatal birthweight following PESA-ICSI. Arch Gynecol Obstet. 2021;305:1233–9.CrossRef

74.

Anger JT, Gilbert BR, Goldstein M. Cryopreservation of sperm: indications, methods and results. J Urol. 2003;170(4):1079–84. https://doi.org/10.1097/01.ju.0000084820.98430.b8.CrossRefPubMed

75.

Wyck S, Herrera C, Requena CE, Bittner L, Hajkova P, Bollwein H, et al. Oxidative stress in sperm affects the epigenetic reprogramming in early embryonic development. Epigenetics Chromatin. 2018;11(1):1–17. https://doi.org/10.1186/s13072-018-0224-y.CrossRef

76.

Chao S, Li J, Jin X, Tang H, Wang G, Gao G. Epigenetic reprogramming of embryos derived from sperm frozen at −20 °C. Sci China Life Sci. 2012;55(4):349–57. https://doi.org/10.1007/s11427-012-4309-8.CrossRefPubMed

77.

Choux C, Petazzi P, Sanchez-Delgado M, Hernandez Mora JR, Monteagudo A, Sagot P, et al. The hypomethylation of imprinted genes in IVF/ICSI placenta samples is associated with concomitant changes in histone modifications. Epigenetics. 2020;15(12):1386–95. https://doi.org/10.1080/15592294.2020.1783168.CrossRefPubMedPubMedCentral

78.

El Hajj N, Haertle L, Dittrich M, Denk S, Lehnen H, Hahn T, et al. DNA methylation signatures in cord blood of ICSI children. Hum Reprod. 2017;32(8):1761–9. https://doi.org/10.1093/humrep/dex209.CrossRefPubMedPubMedCentral

79.

Donkena KV, Young CY, Tindall DJ. Oxidative stress and DNA methylation in prostate cancer. Obstet Gynecol Int. 2010. https://doi.org/10.1155/2010/302051.

80.

Guillaumet-Adkins A, Yañez Y, Peris-Diaz MD, Calabria I, Palanca-Ballester C, Sandoval J. Epigenetics and oxidative stress in aging. Oxidative Med Cell Longev. 2017. https://doi.org/10.1155/2017/9175806.

81.

Lim S-O, Gu J-M, Kim MS, Kim H-S, Park YN, Park CK, et al. Epigenetic changes induced by reactive oxygen species in hepatocellular carcinoma: methylation of the E-cadherin promoter. Gastroenterology. 2008;135(6):2128–40.e8. https://doi.org/10.1053/j.gastro.2008.07.027.CrossRefPubMed

82.

Kopeika J, Thornhill A, Khalaf Y. The effect of cryopreservation on the genome of gametes and embryos: principles of cryobiology and critical appraisal of the evidence. Hum Reprod Update. 2015;21(2):209–27. https://doi.org/10.1093/humupd/dmu063.CrossRefPubMed

Titel: Sperm cryopreservation and DNA methylation: possible implications for ART success and the health of offspring
verfasst von: Zahra Khosravizadeh
Kajal Khodamoradi
Zahra Rashidi
Malihe Jahromi
Elham Shiri
Ensieh Salehi
Ali Talebi
Publikationsdatum: 17.06.2022
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 8/2022
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-022-02545-6

Multi-drug-resistant chronic endometritis in infertile women with repeated implantation failure: trend over the decade and pilot study for third-line oral antibiotic treatment

Reproductive Physiology and Disease

The effect of mTOR activation and PTEN inhibition on human primordial follicle activation in ovarian tissue culture

Fertility Preservation

Does antibiotic therapy for chronic endometritis improve clinical outcomes of patients with recurrent implantation failure in subsequent IVF cycles? A systematic review and meta-analysis

Review

Adaptive data-driven models to best predict the likelihood of live birth as the IVF cycle moves on and for each embryo transfer

Open Access
Assisted Reproduction Technologies

Impact of infertility duration on male sexual function and mental health

Reproductive Physiology and Disease

Fertility preservation practices for female oncofertility differ significantly across the USA: results of a survey of SREI members

Fertility Preservation

Neu im Fachgebiet Gynäkologie und Geburtshilfe

Keine eingeschränkten Kassenleistungen bei Schwangerschaft nach IVF

Medikamente in Schwangerschaft und Stillzeit
Nachrichten

Nach privat bezahlter In-vitro-Fertilisation muss die gesetzliche Krankenkasse ein Arzneimittel zum Erhalt der Schwangerschaft bezahlen, so ein Urteil des Sozialgerichts in München.

Medikamente verändern wohl Nährstoffzusammensetzung der Muttermilch

Medikamente in Schwangerschaft und Stillzeit
Nachrichten

Einige Medikamente wie selektive Serotonin-Wiederaufnahmehemmer können offenbar die Makronährstoffzusammensetzung der Muttermilch verändern. Das birgt möglicherweise gesundheitliche Risiken für manche gestillte Kinder.

CDK4/6-Inhibitoren bei Brustkrebs in die Zweitlinie aufschieben?

10.01.2025
Mammakarzinom
Nachrichten

Ergebnisse einer Phase-III-Studie sprechen dafür, dass die Behandlung mit CDK4/6-Inhibitoren bei fortgeschrittenem HR-positivem, HER2-negativem Brustkrebs auch auf die Zweitlinie verschoben werden könnte, ohne die onkologischen Ergebnisse zu kompromittieren.

Cannabisextrakt verbessert Antiemese bei Chemotherapie

10.01.2025
Antiemetische Therapie
Nachrichten

Sprechen Krebskranke auf die übliche Antiemese während einer Chemotherapie nicht ausreichend an, lohnt sich möglicherweise eine Behandlung mit Cannabisextrakt. In einer Phase-2/3-Studie ließ sich die antiemetische Response mit einem solchen Extrakt erheblich verbessern.

Update Gynäkologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert – ganz bequem per eMail.

Newsletter bestellen

Springer Medizin

Sperm cryopreservation and DNA methylation: possible implications for ART success and the health of offspring

Abstract

Weitere Artikel der Ausgabe 8/2022

Multi-drug-resistant chronic endometritis in infertile women with repeated implantation failure: trend over the decade and pilot study for third-line oral antibiotic treatment

The effect of mTOR activation and PTEN inhibition on human primordial follicle activation in ovarian tissue culture

Does antibiotic therapy for chronic endometritis improve clinical outcomes of patients with recurrent implantation failure in subsequent IVF cycles? A systematic review and meta-analysis

Adaptive data-driven models to best predict the likelihood of live birth as the IVF cycle moves on and for each embryo transfer

Impact of infertility duration on male sexual function and mental health

Fertility preservation practices for female oncofertility differ significantly across the USA: results of a survey of SREI members

Neu im Fachgebiet Gynäkologie und Geburtshilfe

Keine eingeschränkten Kassenleistungen bei Schwangerschaft nach IVF

Medikamente verändern wohl Nährstoffzusammensetzung der Muttermilch

CDK4/6-Inhibitoren bei Brustkrebs in die Zweitlinie aufschieben?

Cannabisextrakt verbessert Antiemese bei Chemotherapie

Update Gynäkologie

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten