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Neuroscience Bulletin
Erschienen in:

08.07.2024 | Protocol

STED Imaging of Vesicular Endocytosis in the Synapse

verfasst von: Shaoqin Hu, Zhenli Xie, Bianbian Wang, Yang Chen, Zexin Jing, Ying Hao, Jingyu Yao, Xuanang Wu, Jingxiao Huo, Anqi Wei, Yuhao Qin, Nan Dong, Chaowen Zheng, Qian Song, Jiangang Long, Xinjiang Kang, Changhe Wang, Huadong Xu

Erschienen in: Neuroscience Bulletin | Ausgabe 9/2024

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Abstract

Endocytosis is a fundamental biological process that couples exocytosis to maintain the homeostasis of the plasma membrane and sustained neurotransmission. Super-resolution microscopy enables optical imaging of exocytosis and endocytosis in live cells and makes an essential contribution to understanding molecular mechanisms of endocytosis in neuronal somata and other types of cells. However, visualization of exo-endocytic events at the single vesicular level in a synapse with optical imaging remains a great challenge to reveal mechanisms governing the synaptic exo-endocytotic coupling. In this protocol, we describe the technical details of stimulated emission depletion (STED) imaging of synaptic endocytosis at the single-vesicle level, from sample preparation and microscopy calibration to data acquisition and analysis.
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Metadaten
Titel
STED Imaging of Vesicular Endocytosis in the Synapse
verfasst von
Shaoqin Hu
Zhenli Xie
Bianbian Wang
Yang Chen
Zexin Jing
Ying Hao
Jingyu Yao
Xuanang Wu
Jingxiao Huo
Anqi Wei
Yuhao Qin
Nan Dong
Chaowen Zheng
Qian Song
Jiangang Long
Xinjiang Kang
Changhe Wang
Huadong Xu
Publikationsdatum
08.07.2024
Verlag
Springer Nature Singapore
Erschienen in
Neuroscience Bulletin / Ausgabe 9/2024
Print ISSN: 1673-7067
Elektronische ISSN: 1995-8218
DOI
https://doi.org/10.1007/s12264-024-01254-7

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