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Erschienen in: Brain Structure and Function 4/2005

01.04.2005 | Original Article

Structural development of PGP9.5-immunopositive myenteric plexus in embryonic rats

verfasst von: Akihide Tanano, Yoshinori Hamada, Shoichiroh Takamido, Yosky Kataoka, Jun Watanabe, Yasuo Kamiyama, Hisao Yamada

Erschienen in: Brain Structure and Function | Ausgabe 4/2005

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Abstract

To investigate relationships between changes in the 3-dimensional structure of the myenteric plexus and the time at which functional movement of intestine begins in mammalian embryos, whole mounts of embryonic rat intestine were examined under confocal laser scanning microscopy on spacer-equipped glass slides after immunostaining with antiprotein gene product 9.5 antibody. At embryonic day 12.5, very few nerve cells were scattered throughout the small intestine, but no immunostained structures were apparent on the anal side of the large intestine. At embryonic day 13.5, immunostained fibers appeared on the oral side of the large intestine. Nerve cells and associated fiber bundles formed neuronal networks with large meshes in both intestines. Marked increases in number of nerve fibers and decreases in mesh size were seen in the small intestine between embryonic days 13.5 and 15.5. Similar changes were found in the large intestine between embryonic days 13.5 and 16.5. After embryonic day 16.5, nerve cells were arranged parallel to circular muscle fibers, and networks formed by cell fibers elongated until the neonatal period in both intestines. Meconium passed through the large intestine from embryonic day 17.5. Thin fiber bundles extended from the ganglion to the inner side of the myenteric layer, parallel (and occasionally extending) to the circular muscle fibers. Formation of nerve fiber networks and arrangement of nerve cells parallel to circular muscle fibers probably relate to movement coordination for inner circular muscle fibers in the intestinal wall, and development of this neural network may be important for acquiring intestinal movements before birth.
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Metadaten
Titel
Structural development of PGP9.5-immunopositive myenteric plexus in embryonic rats
verfasst von
Akihide Tanano
Yoshinori Hamada
Shoichiroh Takamido
Yosky Kataoka
Jun Watanabe
Yasuo Kamiyama
Hisao Yamada
Publikationsdatum
01.04.2005
Erschienen in
Brain Structure and Function / Ausgabe 4/2005
Print ISSN: 1863-2653
Elektronische ISSN: 1863-2661
DOI
https://doi.org/10.1007/s00429-005-0453-2

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