Background
Methods
Experimental animals
Preparation of JDHY granules
Components (Latin name) | Components (English name) | Family |
---|---|---|
Artemisia capillaris Thunb
|
Capillary Wormwood Herb
|
Asteraceae
|
Paeonia veitchii Lynch
|
Radix paeoniae rubrathe
|
Ranunculaceae
|
Rheum palmatum L
|
Rheum officinale
|
Polygonaceae
|
Hedyotis diffusa Willd
|
Oldenlandia diffusa
|
Rubiaceae
|
Curcuma wenyujin Y.H. Chen et C.Ling
|
Radix Curcumae
|
Zingiberaceae
|
Acorus tatarinowii Schott
|
Acorus gramineus
|
Araceae
|
Experimental design
Clinical observation, bodyweight, and food consumption
Hematology and serum biochemistry
Organ coefficient
Histopathology
Statistical analysis
Results
Clinical signs, food consumption, and body weight
Dose(g/kg/d) | 0 | 80 | 100 | 130 |
---|---|---|---|---|
Males | ||||
1 week | 7.32 ± 0.67a
| 7.34 ± 0.39 | 7.42 ± 0.53 | 7.56 ± 0.44 |
2 week | 7.65 ± 0.24 | 7.26 ± 0.48 | 7.42 ± 0.96 | 7.54 ± 0.86 |
3 week | 7.37 ± 0.50 | 7.71 ± 0.38 | 7.70 ± 0.27 | 7.64 ± 0.37 |
4 week | 7.43 ± 0.40 | 7.58 ± 0.20 | 7.33 ± 0.24 | 7.69 ± 0.18 |
5 week | 8.48 ± 0.77 | 8.07 ± 0.59 | 7.89 ± 0.57 | 8.65 ± 0.88 |
6 week | 8.91 ± 0.31 | 8.79 ± 0.40 | 8.63 ± 0.32 | 8.74 ± 0.26 |
7 week | 8.96 ± 0.11 | 9.03 ± 0.20 | 8.94 ± 0.18 | 9.14 ± 0.19 |
8 week | 9.00 ± 0.21 | 9.45 ± 0.20 | 9.02 ± 0.11 | 9.23 ± 0.34 |
9 week | 8.70 ± 0.17 | 8.91 ± 0.16 | 8.90 ± 0.21 | 8.92 ± 0.19 |
10 week | 8.75 ± 0.27 | 9.06 ± 0.27 | 9.02 ± 0.27 | 9.07 ± 0.24 |
11 week | 8.99 ± 0.15 | 9.19 ± 0.18 | 9.13 ± 0.25 | 9.15 ± 0.24 |
12 week | 8.83 ± 0.10 | 8.89 ± 0.21 | 8.92 ± 0.15 | 8.97 ± 0.13 |
13 week | 8.94 ± 0.19 | 8.94 ± 0.11 | 9.03 ± 0.12 | 8.97 ± 0.12 |
Dose (g/kg/d) | 0 | 80 | 100 | 130 |
---|---|---|---|---|
Females | ||||
1 week | 6.34 ± 0.26a
| 6.53 ± 0.17 | 6.79 ± 0.57 | 6.14 ± 0.62 |
2 week | 6.95 ± 0.36 | 6.68 ± 0.41 | 6.94 ± 0.46 | 6.76 ± 0.49 |
3 week | 6.77 ± 0.42 | 6.84 ± 0.58 | 7.32 ± 0.84 | 6.78 ± 0.49 |
4 week | 7.64 ± 0.46 | 7.26 ± 0.30 | 7.57 ± 0.41 | 7.26 ± 0.20 |
5 week | 8.37 ± 0.65 | 8.10 ± 0.51 | 7.73 ± 0.46 | 7.86 ± 0.51 |
6 week | 8.59 ± 0.42 | 8.17 ± 0.42 | 8.21 ± 0.24 | 8.17 ± 0.61 |
7 week | 8.43 ± 0.41 | 8.49 ± 0.41 | 8.21 ± 0.53 | 8.34 ± 0.31 |
8 week | 8.52 ± 0.35 | 8.23 ± 0.20 | 8.72 ± 0.25 | 8.09 ± 0.37 |
9 week | 8.55 ± 0.38 | 8.45 ± 0.38 | 8.63 ± 0.21 | 8.19 ± 0.20 |
10 week | 8.58 ± 0.18 | 8.60 ± 0.19 | 8.45 ± 0.16 | 8.50 ± 0.16 |
11 week | 8.31 ± 0.23 | 8.64 ± 0.32 | 8.32 ± 0.33 | 8.12 ± 0.16 |
12 week | 8.58 ± 0.22 | 8.48 ± 0.24 | 8.36 ± 0.35 | 8.41 ± 0.08 |
13 week | 8.48 ± 0.17 | 8.30 ± 0.13 | 8.59 ± 0.36 | 8.38 ± 0.35 |
Dose (g/kg/d) | 0 | 80 | 100 | 130 |
---|---|---|---|---|
Males | ||||
0 week | 122.3 ± 6.47a
| 122.2 ± 7.39 | 122.1 ± 7.24 | 118.7 ± 5.92 |
1 week | 19.2 ± 5.10 | 17.6 ± 3.83 | 18.2 ± 4.81 | 19.8 ± 5.97 |
2 week | 37.7 ± 8.65 | 37.7 ± 5.75 | 36.8 ± 8.30 | 39.3 ± 9.63 |
3 week | 55.7 ± 9.00 | 56.3 ± 4.95 | 54.9 ± 7.97 | 55.9 ± 8.22 |
4 week | 77.4 ± 9.50 | 77.1 ± 8.50 | 77.2 ± 12.31 | 77.6 ± 10.68 |
5 week | 98.4 ± 11.56 | 96.4 ± 7.670 | 100.5 ± 10.74 | 98.9 ± 11.13 |
6 week | 115.1 ± 15.83 | 114.4 ± 8.47 | 116.9 ± 15.10 | 115.1 ± 13.71 |
7 week | 130.7 ± 15.61 | 119.9 ± 25.50 | 130.9 ± 15.41 | 128.1 ± 12.65 |
8 week | 150.9 ± 19.60 | 144.5 ± 13.73 | 142.3 ± 24.12 | 147.4 ± 14.82 |
9 week | 164.0 ± 17.64 | 158.1 ± 12.94 | 156.1 ± 23.93 | 160.9 ± 14.76 |
10 week | 180.2 ± 15.36 | 173.9 ± 12.50 | 173.7 ± 22.06 | 178.8 ± 11.27 |
11 week | 197.5 ± 14.54 | 189.3 ± 12.35 | 190.0 ± 22.71 | 196.5 ± 11.65 |
12 week | 214.1 ± 16.34 | 203.2 ± 14.56 | 206.7 ± 22.71 | 207.3 ± 26.31 |
13 week | 228.7 ± 16.53 | 217.3 ± 17.04 | 218.2 ± 21.72 | 228.2 ± 16.99 |
Dose (g/kg/d) | 0 | 80 | 100 | 130 |
---|---|---|---|---|
Females | ||||
0 week | 109.0 ± 5.59a
| 109.7 ± 7.70 | 110.3 ± 6.37 | 109.5 ± 6.12 |
1 week | 19.5 ± 6.50 | 18.3 ± 4.93 | 15.8 ± 4.23 | 19.2 ± 5.06 |
2 week | 38.7 ± 10.08 | 37.1 ± 7.84 | 34.7 ± 5.75 | 35.9 ± 7.75 |
3 week | 54.9 ± 7.06 | 56.3 ± 6.63 | 54.1 ± 4.17 | 54.5 ± 8.63 |
4 week | 75.4 ± 11.27 | 76.7 ± 11.98 | 75.3 ± 7.060 | 75.5 ± 11.17 |
5 week | 95.0 ± 11.59 | 99.5 ± 10.85 | 94.2 ± 4.620 | 95.4 ± 13.66 |
6 week | 113.1 ± 13.39 | 117.0 ± 13.33 | 113.5 ± 6.20 | 113.7 ± 16.78 |
7 week | 125.9 ± 11.27 | 130.5 ± 14.74 | 118.2 ± 24.31 | 121.4 ± 26.69 |
8 week | 144.1 ± 14.37 | 141.7 ± 24.10 | 142.4 ± 11.54 | 147.7 ± 20.69 |
9 week | 157.6 ± 14.26 | 156.2 ± 23.75 | 157.1 ± 11.43 | 159.8 ± 20.17 |
10 week | 174.7 ± 12.64 | 173.1 ± 21.59 | 173.0 ± 12.72 | 176.8 ± 20.38 |
11 week | 192.4 ± 13.75 | 188.7 ± 22.59 | 188.5 ± 12.97 | 193.9 ± 19.52 |
12 week | 202.1 ± 26.58 | 204.5 ± 21.15 | 201.2 ± 15.43 | 204.6 ± 29.79 |
13 week | 221.7 ± 19.64 | 216.2 ± 23.10 | 215.1 ± 16.52 | 225.2 ± 20.75 |
Hematology and serum chemistry
Dose (g/kg/d) | 0 | 80 | 100 | 130 |
---|---|---|---|---|
Males | ||||
WBC(109/L) | 7.91 ± 1.89a
| 7.65 ± 1.55 | 8.42 ± 1.74 | 8.03 ± 1.57 |
LYM(109/L) | 3.29 ± 1.13 | 3.77 ± 0.97 | 4.35 ± 1.98 | 4.20 ± 1.97 |
LYM%(%) | 42.85 ± 13.78 | 49.99 ± 11.63 | 50.28 ± 19.21 | 50.44 ± 18.91 |
RC(%) | 18.60 ± 3.27 | 18.50 ± 2.22 | 18.90 ± 3.21 | 18.30 ± 3.94 |
MID(109/L) | 0.92 ± 0.39 | 1.15 ± 0.34 | 1.29 ± 0.42 | 1.19 ± 0.44 |
MID(%) | 11.36 ± 3.70 | 15.27 ± 4.89 | 15.83 ± 6.01 | 14.58 ± 4.58 |
PLT(109/L) | 513.40 ± 103.78 | 505.50 ± 135.51 | 491.00 ± 131.84 | 507.00 ± 115.41 |
GRAN(109/L) | 3.70 ± 1.58 | 2.73 ± 1.34 | 2.78 ± 1.12 | 2.64 ± 1.54 |
GRAN(%) | 45.79 ± 13.22 | 34.74 ± 11.55 | 33.89 ± 14.58 | 34.98 ± 21.28 |
RBC(1012/L) | 5.73 ± 1.63 | 6.06 ± 1.84 | 6.37 ± 1.46 | 6.31 ± 1.71 |
HCT(%) | 42.45 ± 12.07 | 39.58 ± 15.08 | 45.74 ± 10.67 | 43.04 ± 15.63 |
MCV(fl) | 66.76 ± 10.64 | 67.33 ± 7.83 | 67.91 ± 10.39 | 69.79 ± 8.39 |
HGB(g/L) | 128.90 ± 24.14 | 131.20 ± 25.37 | 133.20 ± 23.90 | 126.70 ± 23.37 |
MCH(pg) | 18.13 ± 1.77 | 18.79 ± 1.76 | 18.12 ± 1.09 | 18.48 ± 1.57 |
MCHC(g/L) | 274.70 ± 9.35 | 280.00 ± 9.58 | 279.70 ± 9.31 | 282.70 ± 9.88 |
Dose (g/kg/d) | 0 | 80 | 100 | 130 |
---|---|---|---|---|
Females | ||||
WBC(109/L) | 7.91 ± 1.89a
| 8.94 ± 1.25 | 7.44 ± 2.08 | 8.37 ± 2.10 |
LYM(109/L) | 3.42 ± 1.29 | 4.05 ± 0.97 | 3.58 ± 1.36 | 4.11 ± 1.44 |
LYM%(%) | 46.59 ± 13.74 | 46.04 ± 11.71 | 47.17 ± 12.50 | 47.89 ± 11.92 |
RC(%) | 16.40 ± 2.06 | 16.30 ± 2.11 | 16.80 ± 2.34 | 16.90 ± 2.07 |
MID(109/L) | 1.31 ± 0.61 | 1.27 ± 0.313 | 1.12 ± 0.24 | 1.36 ± 0.31 |
MID(%) | 17.61 ± 5.48 | 14.19 ± 2.90 | 15.82 ± 4.41 | 16.73 ± 4.04 |
PLT(109/L) | 493.00 ± 122.40 | 517.30 ± 169.72 | 511.00 ± 180.39 | 514.10 ± 130.51 |
GRAN(109/L) | 2.69 ± 1.18 | 3.62 ± 1.45 | 2.74 ± 1.08 | 2.90 ± 0.90 |
GRAN(%) | 35.80 ± 11.97 | 39.76 ± 11.84 | 37.01 ± 10.72 | 35.39 ± 10.62 |
RBC(1012/L) | 5.55 ± 1.70 | 5.92 ± 1.79 | 5.77 ± 1.37 | 6.01 ± 1.28 |
HCT(%) | 44.45 ± 12.36 | 43.62 ± 12.95 | 44.01 ± 13.52 | 59.29 ± 12.65 |
MCV(fl) | 60.27 ± 10.80 | 64.38 ± 13.58 | 65.98 ± 13.23 | 69.79 ± 8.39 |
HGB(g/L) | 132.10 ± 32.01 | 131.50 ± 23.08 | 135.50 ± 23.33 | 134.20 ± 28.76 |
MCH(pg) | 18.70 ± 1.78 | 18.74 ± 1.44 | 18.50 ± 1.71 | 18.38 ± 1.23 |
MCHC(g/L) | 280.20 ± 9.61 | 276.00 ± 8.61 | 274.20 ± 8.06 | 277.50 ± 6.46 |
Dose (g/kg/d) | 0 | 80 | 100 | 130 |
---|---|---|---|---|
Males | ||||
AST(U/L) | 217.31 ± 45.65 | 220.68 ± 66.49 | 219.30 ± 86.66 | 196.71 ± 77.69 |
ALT(U/L) | 61.57 ± 9.09a
| 61.62 ± 10.59 | 56.86 ± 10.71 | 58.01 ± 12.24 |
ALP(U/L) | 221.68 ± 88.98 | 239.23 ± 64.49 | 242.42 ± 81.93 | 266.23 ± 82.01 |
TP(g/L) | 85.04 ± 4.67 | 82.7 ± 06.51 | 80.61 ± 6.84 | 86.03 ± 5.78 |
ALB(g/L) | 42.04 ± 4.63 | 41.80 ± 4.78 | 40.84 ± 5.50 | 38.76 ± 5.31 |
TBIL(umol/L) | 7.98 ± 2.34 | 8.30 ± 2.54 | 7.91 ± 2.30 | 7.86 ± 3.32 |
CHOL(mmol/L) | 1.42 ± 0.45 | 1.64 ± 0.49 | 1.44 ± 0.46 | 1.53 ± 0.33 |
TG(mmol/L) | 0.33 ± 0.11 | 0.35 ± 0.11 | 0.36 ± 0.08 | 0.38 ± 0.08 |
GLU(mmol/L) | 7.06 ± 0.97 | 7.43 ± 0.74 | 7.33 ± 0.71 | 7.65 ± 0.91 |
BUN(mmol/L) | 6.80 ± 1.11 | 6.78 ± 1.64 | 6.46 ± 1.41 | 6.47 ± 1.61 |
Cr(umol/L) | 97.32 ± 15.24 | 97.65 ± 15.57 | 99.23 ± 15.67 | 98.54 ± 13.81 |
CK(U/L) | 155.05 ± 25.48 | 147.16 ± 23.52 | 153.79 ± 26.00 | 150.48 ± 24.85 |
Na+(mmol/L) | 150.29 ± 17.72 | 143.53 ± 15.43 | 141.36 ± 18.28 | 145.52 ± 21.69 |
K+(mmol/L) | 4.59 ± 0.93 | 5.07 ± 1.31 | 4.96 ± 1.07 | 5.01 ± 0.84 |
Cl−(mmol/) | 110.30 ± 6.30 | 105.50 ± 7.89 | 105.40 ± 6.93 | 108.00 ± 7.07 |
Dose (g/kg/d) | 0 | 80 | 100 | 130 |
---|---|---|---|---|
Females | ||||
AST(U/L) | 208.34 ± 47.04a
| 212.76 ± 79.64 | 217.31 ± 70.60 | 212.25 ± 89.47 |
ALT(U/L) | 60.57 ± 10.31 | 60.10 ± 11.25 | 58.33 ± 11.02 | 61.53 ± 11.47 |
ALP(U/L) | 237.88 ± 86.37 | 238.82 ± 78.02 | 227.31 ± 66.74 | 232.38 ± 85.91 |
TP(g/L) | 84.12 ± 4.34 | 83.25 ± 8.49 | 80.47 ± 6.27 | 81.14 ± 5.41 |
ALB(g/L) | 41.94 ± 5.24 | 42.61 ± 4.70 | 41.98 ± 5.82 | 41.43 ± 6.42 |
TBIL(umol/L) | 8.23 ± 2.46 | 6.80 ± 2.88 | 8.59 ± 2.99 | 9.63 ± 3.09 |
CHOL(mmol/L) | 1.49 ± 0.52 | 1.61 ± 0.49 | 1.58 ± 0.52 | 1.54 ± 0.37 |
TG(mmol/L) | 0.32 ± 0.07 | 0.36 ± 0.11 | 0.37 ± 0.09 | 0.35 ± 0.07 |
GLU(mmol/L) | 7.35 ± 0.72 | 7.20 ± 0.64 | 7.42 ± 0.61 | 7.57 ± 0.92 |
BUN(mmol/L) | 6.51 ± 1.34 | 6.24 ± 1.36 | 6.35 ± 1.58 | 6.44 ± 1.18 |
Cr(umol/L) | 98.63 ± 11.70 | 95.38 ± 15.75 | 96.72 ± 12.43 | 97.71 ± 12.70 |
CK(U/L) | 155.77 ± 28.07 | 150.13 ± 26.80 | 149.74 ± 26.82 | 144.18 ± 28.09 |
Na+(mmol/L) | 145.14 ± 17.59 | 148.75 ± 13.95 | 146.38 ± 11.86 | 144.94 ± 19.28 |
K+(mmol/L) | 4.81 ± 0.92 | 5.34 ± 0.90 | 5.23 ± 1.12 | 4.93 ± 1.03 |
Cl−(mmol/L) | 108.20 ± 5.95 | 105.80 ± 8.25 | 106.10 ± 8.55 | 107.30 ± 9.20 |
Organ coefficient
Dose (g/kg/d) | 0 | 80 | 100 | 130 |
---|---|---|---|---|
Males | ||||
Heart | 0.38 ± 0.051a
| 0.36 ± 0.051 | 0.36 ± 0.04 | 0.37 ± 0.06 |
Liver | 3.44 ± 0.26 | 3.75 ± 0.57 | 3.51 ± 0.63 | 3.40 ± 0.53 |
Spleen | 0.22 ± 0.05 | 0.25 ± 0.03 | 0.34 ± 0.03 | 0.22 ± 0.04 |
Lung | 0.68 ± 0.14 | 0.62 ± 0.05 | 0.70 ± 0.05 | 0.71 ± 0.11 |
Kidney | 0.62 ± 0.14 | 0.66 ± 0.06 | 0.71 ± 0.07 | 0.70 ± 0.10 |
Brain | 0.48 ± 0.09 | 0.56 ± 0.09 | 0.52 ± 0.08 | 0.49 ± 0.09 |
Thymus | 0.10 ± 0.04 | 0.09 ± 0.02 | 0.10 ± 0.03 | 0.10 ± 0.03 |
Renicapsule | 0.02 ± 0.02 | 0.02 ± 0.01 | 0.02 ± 0.01 | 0.01 ± 0.03 |
Orchis | 0.51 ± 0.16 | 0.49 ± 0.20 | 0.41 ± 0.14 | 0.49 ± 0.14 |
Epididymis | 0.20 ± 0.09 | 0.27 ± 0.07 | 0.26 ± 0.05 | 0.24 ± 0.09 |
Dose (g/kg/d) | 0 | 80 | 100 | 130 |
---|---|---|---|---|
Females | ||||
Heart | 0.31 ± 0.03a
| 0.28 ± 0.03 | 0.27 ± 0.04 | 0.33 ± 0.09 |
Liver | 3.27 ± 0.44 | 3.27 ± 0.45 | 3.18 ± 0.67 | 3.26 ± 0.38 |
Spleen | 0.30 ± 0.12 | 0.34 ± 0.11 | 0.35 ± 0.12 | 0.35 ± 0.11 |
Lung | 0.55 ± 0.11 | 0.61 ± 0.26 | 0.63 ± 0.28 | 0.73 ± 0.37 |
Kidney | 0.61 ± 0.08 | 0.64 ± 0.08 | 0.64 ± 0.08 | 0.60 ± 0.07 |
Brain | 0.51 ± 0.07 | 0.47 ± 0.04 | 0.53 ± 0.09 | 0.45 ± 0.07 |
Thymus | 0.17 ± 0.08 | 0.22 ± 0.06 | 0.22 ± 0.08 | 0.18 ± 0.11 |
Renicapsule | 0.02 ± 0.006 | 0.02 ± 0.004 | 0.02 ± 0.005 | 0.02 ± 0.006 |
Uterus | 0.23 ± 0.06 | 0.24 ± 0.03 | 0.25 ± 0.12 | 0.21 ± 0.05 |
Oarium | 0.06 ± 0.01 | 0.07 ± 0.02 | 0.05 ± 0.01 | 0.06 ± 0.02 |