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01.12.2014 | Case report | Ausgabe 1/2014 Open Access

Journal of Medical Case Reports 1/2014

Successful diagnosis of tuberculous lymphadenitis by loop-mediated isothermal amplification of cutaneous samples from an ulcerated surface lesion: a case report

Journal of Medical Case Reports > Ausgabe 1/2014
Shuichi Kawano, Takuya Maeda, Junichi Watanabe, Yuji Fujikura, Kei Mikita, Yu Hara, Soichiro Kanoh, Fumihiko Kimura, Yasushi Miyahira, Akihiko Kawana
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1752-1947-8-254) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

Skaw conceived the study, participated in its design and coordination and helped draft the manuscript. TM was directly involved in the overall care of the patient and drafted the manuscript. JW, YF, KM, YH, SKan, FK, YM and AK were involved in data investigation and the patient’s treatment. All authors read and approved the final manuscript.



Tuberculous lymphadenitis is the most frequent form of extrapulmonary tuberculous. Although nucleic acid amplification assays such as polymerase chain reaction have recently become mainstream techniques for diagnosing tuberculous lymphadenitis, they are still not routinely performed in developing countries because of their high costs and complicated procedures.

Case presentation

We describe a case of tuberculous lymphadenitis in a 79-year-old Japanese man who had been on continuous hemodialysis for end-stage renal disease. We employed loop-mediated isothermal amplification and the procedure for ultrarapid extraction to develop a fast and easy-to-perform procedure for diagnosing tuberculous lymphadenitis.


The commercially available loop-mediated isothermal amplification assay kit and a rapid purification procedure enabled us to identify and amplify a Mycobacterium tuberculosis–specific gene within just 1.5 hours.

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