nach oben

Journal of Assisted Reproduction and Genetics

Erschienen in:

02.10.2017 | Gamete Biology

Successful intracytoplasmic sperm injection with testicular spermatozoa from a man with multiple morphological abnormalities of the sperm flagella: a case report

verfasst von: Shenmin Yang, Liang Gao, Wei Wang, Jie Ding, Yongle Xu, Hong Li

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 2/2018

Einloggen, um Zugang zu erhalten

Abstract

Purpose

The purpose of this study is to analyze the sperm morphology of a Chinese man affected with multiple morphological abnormalities of the sperm flagella (MMAF) and observe the intracytoplasmic sperm injection (ICSI) outcome.

Methods

A Chinese man was diagnosed with multiple morphological abnormalities of the sperm flagella by semen analysis and electron microscopy. Testicular spermatozoa were injected intracytoplasmically, and the following ICSI results were observed.

Results

All the spermatozoa from his ejaculate were immotile and morphologically abnormal in the flagellum. In transmission electron microscopy assays, most spermatozoa showed disorganized fibrous sheath, accompanied by distortion of various cytoskeletal components, and missing of the central pair microtubules. Testicular sperm was injected to the oocytes in two ICSI cycles, with fertilization rates of 45.5 and 40.0%. Finally, a healthy female infant was delivered at the second ICSI cycle.

Conclusions

Fertilization and pregnancy could be achieved by intracytoplasmic sperm injection, regardless of severe flagellar defects. ICSI is effective for MMAF-affected man, and testicular sperm is an alternative when no motile sperm is available.

Yang SM, Li HB, Wang JX, Shi YC, Cheng HB, Wang W, et al. Morphological characteristics and initial genetic study of multiple morphological anomalies of the flagella in China. Asian J Androl. 2015;17:513–5.CrossRefPubMedPubMedCentral

Ben Khelifa M, Coutton C, Zouari R, Karaouzene T, Rendu J, Bidart M, et al. Mutations in DNAH1, which encodes an inner arm heavy chain dynein, lead to male infertility from multiple morphological abnormalities of the sperm flagella. Am J Hum Genet. 2014;94:95–104.CrossRefPubMedPubMedCentral

Chemes HE, Brugo S, Zanchetti F, Carrere C, Lavieri JC. Dysplasia of the fibrous sheath: an ultrastructural defect of human spermatozoa associated with sperm immotility and primary sterility. Fertil Steril. 1987;48:664–9.CrossRefPubMed

Stalf T, Sanchez R, Kohn FM, Schalles U, Kleinstein J, Hinz V, et al. Pregnancy and birth after intracytoplasmic sperm injection with spermatozoa from a patient with tail stump syndrome. Hum Reprod. 1995;10:2112–4.CrossRefPubMed

Chemes HE, Alvarez SC. Tales of the tail and sperm head aches: changing concepts on the prognostic significance of sperm pathologies affecting the head, neck and tail. Asian J Androl. 2012;14:14–23.CrossRefPubMed

Coutton C, Escoffier J, Martinez G, Arnoult C, Ray PF. Teratozoospermia: spotlight on the main genetic actors in the human. Hum Reprod Update. 2015;21:455–85.CrossRefPubMed

Tang S, Wang X, Li W, Yang X, Li Z, Liu W, et al. Biallelic mutations in CFAP43 and CFAP44 cause male infertility with multiple morphological abnormalities of the sperm flagella. Am J Hum Genet. 2017;100:854–64.CrossRefPubMedPubMedCentral

Olmedo SB, Nodar F, Chillik C, Chemes HE. Successful intracytoplasmic sperm injection with spermatozoa from a patient with dysplasia of the fibrous sheath and chronic respiratory disease. Hum Reprod. 1997;12:1497–9.CrossRefPubMed

Olmedo SB, Rawe VY, Nodar FN, Galaverna GD, Acosta AA, Chemes HE. Pregnancies established through intracytoplasmic sperm injection (ICSI) using spermatozoa with dysplasia of fibrous sheath. Asian J Androl. 2000;2:125–30.PubMed

10.

Yang SM, Yang XY, Ding Y, Li H, Wang W, Liu JY, et al. Intracytoplasmic sperm injection outcomes in Chinese men with multiple morphological abnormalities of sperm flagella. Asian J Androl. 2016;18:809–11.CrossRefPubMed

11.

Racowsky C, Ohno-Machado L, Kim J, Biggers JD. Is there an advantage in scoring early embryos on more than one day. Hum Reprod. 2009;24:2104–13.CrossRefPubMedPubMedCentral

12.

Chemes HE, Olmedo SB, Carrere C, Oses R, Carizza C, Leisner M, et al. Ultrastructural pathology of the sperm flagellum: association between flagellar pathology and fertility prognosis in severely asthenozoospermic men. Hum Reprod. 1998;13:2521–6.CrossRefPubMed

13.

Chemes EH, Rawe YV. Sperm pathology: a step beyond descriptive morphology. Origin, characterization and fertility potential of abnormal sperm phenotypes in infertile men. Hum Reprod Update. 2003;9:405–28.CrossRefPubMed

14.

Linck RW, Chemes H, Albertini DF. The axoneme: the propulsive engine of spermatozoa and cilia and associated ciliopathies leading to infertility. J Assist Reprod Genet. 2016;33:141–56.CrossRefPubMedPubMedCentral

15.

Nordhoff V. How to select immotile but viable spermatozoa on the day of intracytoplasmic sperm injection? An embryologists view. Andrology. 2015;3:156–62.

16.

Ortega C, Verheyen G, Raick D, Camus M, Devroey P, Tournaye H. Absolute asthenozoospermia and ICSI: what are the options. Hum Reprod Update. 2011;17:684–92.CrossRefPubMed

17.

Nagy ZP, Verheyen G, Tournaye H, Van Steirteghem AC. Special applications of intracytoplasmic sperm injection: the influence of sperm count, motility, morphology, source and sperm antibody on the outcome of ICSI. Hum Reprod. 1998;13(Suppl 1):143–54.CrossRefPubMed

18.

Vandervorst M, Tournaye H, Camus M, Nagy ZP, Van Steirteghem A, Devroey P. Patients with absolutely immotile spermatozoa and intracytoplasmic sperm injection. Hum Reprod. 1997;12:2429–33.CrossRefPubMed

19.

Casper RF, Meriano JS, Jarvi KA, Cowan L, Lucato ML. The hypo-osmotic swelling test for selection of viable sperm for intracytoplasmic sperm injection in men with complete asthenozoospermia. Fertil Steril. 1996;65:972–6.CrossRefPubMed

20.

Kordus RJ, Price RL, Davis JM, Whitman-Elia GF. Successful twin birth following blastocyst culture of embryos derived from the immotile ejaculated spermatozoa from a patient with primary ciliary dyskinesia: a case report. J Assist Reprod Genet. 2008;25:437–43.CrossRefPubMedPubMedCentral

21.

Kahraman S, Isik AZ, Vicdan K, Ozgur S, Ozgun OD. A healthy birth after intracytoplasmic sperm injection by using immotile testicular spermatozoa in a case with totally immotile ejaculated spermatozoa before and after Percoll gradients. Hum Reprod. 1997;12:292–3.CrossRefPubMed

22.

Kahraman S, Tasdemir M, Tasdemir I, Vicdan K, Ozgur S, Polat G, et al. Pregnancies achieved with testicular and ejaculated spermatozoa in combination with intracytoplasmic sperm injection in men with totally or initially immotile spermatozoa in the ejaculate. Hum Reprod. 1996;11:1343–6.CrossRefPubMed

23.

Shulman A, Feldman B, Madgar I, Levron J, Mashiach S, Dor J. In-vitro fertilization treatment for severe male factor: the fertilization potential of immotile spermatozoa obtained by testicular extraction. Hum Reprod. 1999;14:749–52.CrossRefPubMed

24.

McLachlan RI, Ishikawa T, Osianlis T, Robinson P, Merriner DJ, Healy D, et al. Normal live birth after testicular sperm extraction and intracytoplasmic sperm injection in variant primary ciliary dyskinesia with completely immotile sperm and structurally abnormal sperm tails. Fertil Steril. 2012;97:313–8.CrossRefPubMed

25.

Ravel C, Chantot-Bastaraud S, Siffroi JP, Escalier D, Antoine JM, Mandelbaum J. Tail stump syndrome associated with chromosomal translocation in two brothers attempting intracytoplasmic sperm injection. Fertil Steril. 2006;86:719.e1–7.CrossRef

26.

Wambergue C, Zouari R, Ben F, Mustapha S, Martinez G, Devillard F, et al. Patients with multiple morphological abnormalities of the sperm flagella due to DNAH1 mutations have a good prognosis following intracytoplasmic sperm injection. Hum Reprod. 2016;31:1164–72.CrossRefPubMed

27.

Favero R, Rizzo F, Baccetti B, Piomboni P. Embryo development, pregnancy and twin delivery after microinjection of ‘stump’ spermatozoa. Andrologia. 1999;31:335–8.CrossRefPubMed

Titel: Successful intracytoplasmic sperm injection with testicular spermatozoa from a man with multiple morphological abnormalities of the sperm flagella: a case report
verfasst von: Shenmin Yang
Liang Gao
Wei Wang
Jie Ding
Yongle Xu
Hong Li
Publikationsdatum: 02.10.2017
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 2/2018
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-017-1057-5

Neu im Fachgebiet Gynäkologie und Geburtshilfe

23.04.2024 | Medikamente in Schwangerschaft und Stillzeit | Nachrichten

Update Gynäkologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert – ganz bequem per eMail.

Newsletter bestellen

Springer Medizin

Successful intracytoplasmic sperm injection with testicular spermatozoa from a man with multiple morphological abnormalities of the sperm flagella: a case report

Abstract

Purpose

Methods

Results

Conclusions

Neu im Fachgebiet Gynäkologie und Geburtshilfe

ICI-Therapie in der Schwangerschaft wird gut toleriert

Weniger postpartale Depressionen nach Esketamin-Einmalgabe

Bei RSV-Impfung vor 60. Lebensjahr über Off-Label-Gebrauch aufklären!

Harnwegsinfektprophylaxe: Es geht auch ohne Antibiotika

Update Gynäkologie

Springer Medizin

Abstract

Purpose

Methods

Results

Conclusions

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 2/2018

Improvement of pregnancy outcome by extending embryo culture in IVF-ET during clinical application

FDA-approved drugs that are spermatotoxic in animals and the utility of animal testing for human risk prediction

The normality of sperm in an infertile man with ring chromosome 15: a case report

Should intrauterine human chorionic gonadotropin infusions ever be used prior to embryo transfer?

Follicle dynamics: visualization and analysis of follicle growth and maturation using murine ovarian tissue culture

A novel oocyte maturation trigger using 1500 IU of human chorionic gonadotropin plus 450 IU of follicle-stimulating hormone may decrease ovarian hyperstimulation syndrome across all in vitro fertilization stimulation protocols

Neu im Fachgebiet Gynäkologie und Geburtshilfe

ICI-Therapie in der Schwangerschaft wird gut toleriert

Weniger postpartale Depressionen nach Esketamin-Einmalgabe

Bei RSV-Impfung vor 60. Lebensjahr über Off-Label-Gebrauch aufklären!

Harnwegsinfektprophylaxe: Es geht auch ohne Antibiotika

Update Gynäkologie