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Clinical Oral Investigations

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01.03.2015 | Original Article

Supplementary sampling of obturation materials enhances microbial analysis of endodontic treatment failures: a proof of principle study

verfasst von: L. Karygianni, A. C. Anderson, C. Tennert, K. Kollmar, M. J. Altenburger, E. Hellwig, A. Al-Ahmad

Erschienen in: Clinical Oral Investigations | Ausgabe 2/2015

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Abstract

Objectives

Root canal treatment failures often correlate with persistent biomaterial-associated endodontic infections. The aim of the present study was to assess the impact of endodontic obturation material sampling from root canals with posttreatment apical periodontitis on improving standard study protocols.

Materials and methods

Samples from previously filled root canals and their corresponding endodontic filling materials were obtained from five root-filled teeth with posttreatment periradicular lesions. After cultivation, the isolated microorganisms were quantified and biochemically identified. Moreover, clone libraries were constructed after the amplification of bacterial 16S ribosomal DNA (rDNA) from the same samples. DNA from selected clones was sequenced to identify microbial species. Transmission electron microscopy (TEM) aided visualization of the detected bacteria.

Results

Overall, 22 taxa of the phyla Firmicutes, Actinobacteria, and Bacteroidetes were detected in both obturation and root canal samples by culture-dependent and culture-independent methods. Root canal fillings sheltered 17 species (3.30–7.50 × 10³ CFU/ml). Of these, nine were detected solely in the retrieved obturation materials. The reinfected root canals harbored 13 taxa (3.48–7.36 × 10³ CFU/ml). Obligate and facultative anaerobic bacteria prevailed. The number of different species ranged from 1 to 5 within a single sample. Fungi were not detected.

Conclusions

Bacteria can colonize both root canals and endodontic fillings in vivo.

Clinical relevance

Integrating the sampling of obturation materials with standard root canal sample collection offers a clearer insight into the actual microbial flora of reinfected root canals and improves the study protocols of secondary/persistent endodontic infections.

Siqueira JF Jr, Rôças IN (2005) Uncultivated phylotypes and newly named species associated with primary and persistent endodontic infections. J Clin Microbiol 43:3314–3319CrossRefPubMedCentralPubMed

Rôças IN, Siqueira JF Jr (2012) Characterization of microbiota of root canal-treated teeth with posttreatment disease. J Clin Microbiol 50:1721–1724CrossRefPubMedCentralPubMed

Cheung GS, Ho MW (2001) Microbial flora of root canal-treated teeth associated with asymptomatic periapical radiolucent lesions. Oral Microbiol Immunol 16:332–337CrossRefPubMed

Rocas IN, Jung IY, Lee CY, Siqueira JF Jr (2004) Polymerase chain reaction identification of microorganisms in previously root-filled teeth in a South Korean population. J Endod 30:504–508CrossRefPubMed

Siqueira JF Jr, Rocas IN (2004) Polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 97:85–94CrossRefPubMed

Anderson AC, Hellwig E, Vespermann R, Wittmer A, Schmid M, Karygianni L, Al-Ahmad A (2012) Comprehensive analysis of secondary dental root canal infections: a combination of culture and culture-independent approaches reveals new insights. PLoS One 7:e49576CrossRefPubMedCentralPubMed

Pinheiro ET, Gomes BP, Ferraz CC, Sousa EL, Teixeira FB, Souza-Filho FJ (2003) Microorganisms from canals of root-filled teeth with periapical lesions. Int Endod J 36:1–11CrossRefPubMed

Siqueira JF Jr, Rôças IN, Paiva SS, Magalhães KM, Guimarães-Pinto T (2007) Cultivable bacteria in infected root canals as identified by 16S rRNA gene sequencing. Oral Microbiol Immunol 22:266–271CrossRefPubMed

Rolph HJ, Lennon A, Riggio MP, Saunders WP, MacKenzie D et al (2001) Molecular identification of microorganisms from endodontic infections. J Clin Microbiol 39:3282–3289CrossRefPubMedCentralPubMed

10.

Young G, Turner S, Davies JK, Sundqvist G, Figdor D (2007) Bacterial DNA persists for extended periods after cell death. J Endod 33:1417–1420CrossRefPubMed

11.

Sakamoto SM, Siqueira JF Jr, Rocas IN, Benno Y (2008) Molecular analysis of the root canal microbiota associated with endodontic treatment failures. Oral Microbiol Immunol 23:275–281CrossRefPubMed

12.

Subramanian K, Mickel A (2009) Molecular analysis of persistent periradicular lesions and root ends reveals a diverse microbial profile. J Endod 35:950–957CrossRefPubMed

13.

Paiva SS, Siqueira JF Jr, Rôças IN, Carmo FL, Leite DC, Ferreira DC, Rachid CT, Rosado AS (2013) Molecular microbiological evaluation of passive ultrasonic activation as a supplementary disinfecting step: a clinical study. J Endod 39:190–194CrossRefPubMed

14.

Sundqvist G, Figdor D, Persson S, Sjogren U (1998) Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative retreatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 85:86–93CrossRefPubMed

15.

Senges C, Wrbas KT, Altenburger M, Follo M, Spitzmüller B, Wittmer A, Hellwig E, Al-Ahmad A (2011) Bacterial and Candida albicans adhesion on different root canal filling materials and sealers. J Endod 37:1247–1252CrossRefPubMed

16.

George S, Basrani B, Kishen A (2010) Possibilities of gutta-percha-centered infection in endodontically treated teeth: an in vitro study. J Endod 36:1241–1244CrossRefPubMed

17.

Love RM (2002) The effect of tissue molecules on bacterial invasion of dentine. Oral Microbiol Immunol 17:32–37CrossRefPubMed

18.

Takemura N, Noiri Y, Ehara A, Kawahara T, Noguchi N, Ebisu S (2004) Single species biofilm-forming ability of root canal isolates on gutta-percha points. Eur J Oral Sci 112:523–529CrossRefPubMed

19.

Nawal RR, Parande M, Sehgal R, Rao NR, Naik A (2011) A comparative evaluation of 3 root canal filling systems. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 111:387–393CrossRefPubMed

20.

Gomes BP, Vianna ME, Matsumoto CU, Rossi Vde P, Zaia AA, Ferraz CC, Souza Filho FJ (2005) Disinfection of gutta-percha cones with chlorhexidine and sodium hypochlorite. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 100:512–517CrossRefPubMed

21.

Farzaneh M, Abitbol S, Friedman S (2004) Treatment outcome in endodontics: the Toronto study. Phases I and II: Orthograde retreatment. J Endod 30:627–633CrossRefPubMed

22.

Schirrmeister JF, Liebenow AL, Braun G, Wittmer A, Hellwig E et al (2007) Detection and eradication of microorganisms in root-filled teeth associated with periradicular lesions: an in vivo study. J Endod 33:536–540CrossRefPubMed

23.

Schirrmeister JF, Liebenow AL, Pelz K, Wittmer A, Serr A et al (2009) New bacterial compositions in root-filled teeth with periradicular lesions. J Endod 35:169–174CrossRefPubMed

24.

Frank JA, Reich CL, Sharma S, Weisbaum JS, Wilson BA et al (2008) Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes. Appl Environ Microbiol 74:2461–2470CrossRefPubMedCentralPubMed

25.

Vianna ME, Conrads G, Gomes BPFA, Horz HP (2006) Identification and quantification of archaea involved in primary endodontic infections. J Clin Microbiol 44:1274–1282CrossRefPubMedCentralPubMed

26.

Gardes M, Bruns TD (1993) ITSprimers with enhanced specificity for basidiomycetes — application to the identification of mycorrhizae and rusts. Mol Ecol 2:113–118CrossRefPubMed

27.

Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z et al (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402CrossRefPubMedCentralPubMed

28.

Ashelford KE, Chuzhanova NA, Fry JC, Jones AJ, Weightman AJ (2005) At least 1 in 20 16S rRNA sequence records currently held in public repositories is estimated to contain substantial anomalies. Appl Environ Microbiol 71:7724–7736CrossRefPubMedCentralPubMed

29.

Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

30.

Cole JR, Wang Q, Cardenas E, Fish J, Chai B et al (2009) The ribosomal database project: improved alignments and new tools for rRNA analysis. Nucleic Acids Res (Database issue) 37:D141–D145CrossRef

31.

Chen T, Yu WH, Izard J, Baranova OV, Lakshmanan A, Dewhirst FE (2010) The human oral microbiome database: a web accessible resource for investigating oral microbe taxonomic and genomic information. Database (Oxford) Jul 6;2010:baq013

32.

Wahab R, Mishra A, Yun SI, Kim YS, Shin HS (2010) Antibacterial activity of ZnO nanoparticles prepared via non-hydrolytic solution route. Appl Microbiol Biotechnol 87:1917–1925CrossRefPubMed

33.

Hirai VH, da Silva Neto UX, Westphalen VP, Perin CP, Carneiro E, Fariniuk LF (2010) Comparative analysis of leakage in root canal fillings performed with gutta-percha and resilon cones with AH plus and epiphany sealers. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 109:e131–e135CrossRefPubMed

34.

Teixeira CS, Alfredo E, Thomé LH, Gariba-Silva R, Silva-Sousa YT, Sousa-Neto MD (2009) Adhesion of an endodontic sealer to dentin and gutta-percha: shear and push-out bond strength measurements and SEM analysis. J Appl Oral Sci 17:129–135CrossRefPubMed

35.

Shokati B, Tam LE, Santerre JP, Finer Y (2010) Effect of salivary esterase on the integrity and fracture toughness of the dentin–resin interface. J Biomed Mater Res B Appl Biomater 94:230–237PubMed

36.

Roth KA, Friedman S, Lévesque CM, Basrani BR, Finer Y (2012) Microbial biofilm proliferation within sealer-root dentin interfaces is affected by sealer type and aging period. J Endod 38:1253–1256CrossRefPubMedCentralPubMed

37.

Hashimoto M (2010) A review—micromorphological evidence of degradation in resin–dentin bonds and potential preventional solutions. J Biomed Mater Res B Appl Biomater 92:268–280CrossRefPubMed

38.

Richardson N, Mordan NJ, Figueiredo JA, Ng YL, Gulabivala K (2009) Microflora in teeth associated with apical periodontitis: a methodological observational study comparing two protocols and three microscopy techniques. Int Endod J 42:908–921CrossRefPubMed

39.

Pinheiro ET, Gomes BP, Ferraz CC, Teixeira FB, Zaia AA et al (2003) Evaluation of root canal microorganisms isolated from teeth with endodontic failure and their antimicrobial susceptibility. Oral Microbiol Immunol 18:100–103CrossRefPubMed

40.

Nagy E, Urbán E, Becker S, Kostrzewa M, Vörös A, Hunyadkürti J, Nagy I (2013) MALDI-TOF MS fingerprinting facilitates rapid discrimination of phylotypes I, II and III of Propionibacterium acnes. Anaerobe 20:20–26CrossRefPubMed

41.

Borgo F, Ferrario C, Ricci G, Fortina MG (2013) Genotypic intraspecies heterogeneity of Enterococcus italicus: data from dairy environments. J Basic Microbiol 53:20–28CrossRefPubMed

42.

Linares DM, Del Río B, Ladero V, Redruello B, Martín MC, Fernández M, Alvarez MA (2013) The putrescine biosynthesis pathway in Lactococcus lactis is transcriptionally regulated by carbon catabolic repression, mediated by CcpA. Int J Food Microbiol 165:43–50CrossRefPubMed

43.

Noiri Y, Ehara A, Kawahara T, Takemura N, Ebisu S (2002) Participation of bacterial biofilms in refractory and chronic periapical periodontitis. J Endod 28:679–683CrossRefPubMed

44.

Nambu T, Yamane K, Yamanaka T, Mashimo C, Maruyama H, Yoshida M, Hayashi H, Leung KP, Fukushima H (2013) Identification of disulphide stress-responsive extracytoplasmic function sigma factors in Rothia mucilaginosa. Arch Oral Biol 58:681–689CrossRefPubMed

45.

Kermanshahi S, Santerre JP, Cvitkovitch DG, Finer Y (2010) Biodegradation of resin–dentin interfaces increases bacterial microleakage. J Dent Res 89:996–1001CrossRefPubMedCentralPubMed

Titel: Supplementary sampling of obturation materials enhances microbial analysis of endodontic treatment failures: a proof of principle study
verfasst von: L. Karygianni
A. C. Anderson
C. Tennert
K. Kollmar
M. J. Altenburger
E. Hellwig
A. Al-Ahmad
Publikationsdatum: 01.03.2015
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 2/2015
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-014-1231-4

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Supplementary sampling of obturation materials enhances microbial analysis of endodontic treatment failures: a proof of principle study

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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Springer Medizin

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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