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Inflammation Research

Erschienen in:

15.03.2017 | Original Research Paper

Suppression of cytokine production by glucocorticoids is mediated by MKP-1 in human lung epithelial cells

verfasst von: Tiina Keränen, Eeva Moilanen, Riku Korhonen

Erschienen in: Inflammation Research | Ausgabe 5/2017

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Abstract

Mitogen-activated protein kinase phosphatase 1 (MKP-1) expression is induced by inflammatory factors and serves as an endogenous p38 MAPK suppressor to limit inflammatory response. Glucocorticoids are very effective anti-inflammatory drugs and they are used for the treatment of many inflammatory diseases, such as asthma and COPD. We investigated the role of MKP-1 in the inhibition of cytokine production by dexamethasone in human A549 bronchial epithelial cells. We found that dexamethasone increased MKP-1 expression, inhibited p38 MAPK phosphorylation, and suppressed TNF and MIP-3α production in A549 cells. Interestingly, the suppression of p38 MAPK phosphorylation and the inhibition of TNF expression by dexamethasone were attenuated in cells, where MKP-1 expression was silenced by siRNA. In conclusion, these data suggest that dexamethasone increases MKP-1 expression and this results in the suppression of p38 MAPK signaling leading to the inhibition of cytokine production in human bronchial epithelial cells. These results point to the role of MKP-1 as an important factor in the therapeutic effects of glucocorticoids in the treatment of inflammatory lung diseases.

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Titel: Suppression of cytokine production by glucocorticoids is mediated by MKP-1 in human lung epithelial cells
verfasst von: Tiina Keränen
Eeva Moilanen
Riku Korhonen
Publikationsdatum: 15.03.2017
Verlag: Springer International Publishing
Erschienen in: Inflammation Research / Ausgabe 5/2017
Print ISSN: 1023-3830
Elektronische ISSN: 1420-908X
DOI: https://doi.org/10.1007/s00011-017-1028-4

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Suppression of cytokine production by glucocorticoids is mediated by MKP-1 in human lung epithelial cells

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