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European Journal of Trauma and Emergency Surgery

Erschienen in:

01.10.2010 | Original Article

Surface proteins and osteoblast markers: characterization of human adipose tissue-derived osteogenic cells

verfasst von: O. A. Trentz, D. Arikketh, V. Sentilnathan, S. Hemmi, A. E. Handschin, B. de Rosario, P. Mohandas, P. V. A. Mohandas

Erschienen in: European Journal of Trauma and Emergency Surgery | Ausgabe 5/2010

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Abstract

Introduction

In adipose tissue-derived osteogenic cells (ADOC), osteoblast markers and surface proteins were determined and compared with osteoblasts harvested from cancellous bone (OB).

Method

Osteocalcin (OC), core binding factor 1 (CBFA1), collagen type 1 (Coll1), alkaline phosphatase (ALP), nucleostemin (NS), and surface proteins CD 10, CD44, CD 59 and CD 105 were analyzed using RT-PCR, immunofluorescence and Western blot.

Results

Osteocalcin expression was more distinct in OB than in ADOC, but the other markers and surface proteins showed no differences.

Conclusion

These data support the use of adipose tissue for future regenerative medicine; however, further studies are necessary to establish the role of long-term differentiation.

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Titel: Surface proteins and osteoblast markers: characterization of human adipose tissue-derived osteogenic cells
verfasst von: O. A. Trentz
D. Arikketh
V. Sentilnathan
S. Hemmi
A. E. Handschin
B. de Rosario
P. Mohandas
P. V. A. Mohandas
Publikationsdatum: 01.10.2010
Verlag: Urban and Vogel
Erschienen in: European Journal of Trauma and Emergency Surgery / Ausgabe 5/2010
Print ISSN: 1863-9933
Elektronische ISSN: 1863-9941
DOI: https://doi.org/10.1007/s00068-010-0030-0

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Surface proteins and osteoblast markers: characterization of human adipose tissue-derived osteogenic cells

Abstract

Introduction

Method

Results

Conclusion

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Springer Medizin

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Introduction

Method

Results

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