01.10.2010 | Original Article
Surface proteins and osteoblast markers: characterization of human adipose tissue-derived osteogenic cells
O. A. Trentz, D. Arikketh, V. Sentilnathan, S. Hemmi, A. E. Handschin, B. de Rosario, P. Mohandas, P. V. A. Mohandas
European Journal of Trauma and Emergency Surgery
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In adipose tissue-derived osteogenic cells (ADOC), osteoblast markers and surface proteins were determined and compared with osteoblasts harvested from cancellous bone (OB).
Osteocalcin (OC), core binding factor 1 (CBFA1), collagen type 1 (Coll1), alkaline phosphatase (ALP), nucleostemin (NS), and surface proteins CD 10, CD44, CD 59 and CD 105 were analyzed using RT-PCR, immunofluorescence and Western blot.
Osteocalcin expression was more distinct in OB than in ADOC, but the other markers and surface proteins showed no differences.
These data support the use of adipose tissue for future regenerative medicine; however, further studies are necessary to establish the role of long-term differentiation.