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Erschienen in: Journal of Hematology & Oncology 1/2016

Open Access 01.12.2016 | Letter to the Editor

Systematic analysis of overall survival and interactions between tumor mutations and drug treatment

verfasst von: Francesco Gatto, Jens Nielsen

Erschienen in: Journal of Hematology & Oncology | Ausgabe 1/2016

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Abstract

Background

Few exceptional responses in cancer treatment were attributed to a genetic predisposition of the tumor.

Methods

We analyzed a cohort of 3105 patients from 12 different cancer types and systematically sought the existence of a correlation between overall survival and the interaction of 21 antineoplastic treatments with 6 tumor mutations.

Results

We identified a single significant correlation resulting in increased overall survival from temozolomide in lower-grade glioma with IDH1 R132H mutations. The trend could not be attributed to either the treatment or the mutation alone. Univariate and multivariate Cox regression demonstrated that this interaction stood as an independent prognostic predictor of survival.

Conclusion

Our results suggest infrequent instances of exceptional responses ascribable to tumor genomics yet corroborate the existence of an interaction of temozolomide with IDH1 mutations in lower-grade glioma.
Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​s13045-016-0249-2) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare no conflict of interest in relation to the submitted work.

Authors’ contributions

FG conceived and designed the study and performed the analyses; JN supervised the study. All authors approved the manuscript in its final form.

Findings

The cancer genome can elicit sensitivity to certain drugs not specifically designed to target the underlying genetic aberrations. To this end, genomic markers of drug sensitivity have been systematically assessed in cancer cell lines [1, 2]. Ideally, these markers can identify patients who may better benefit from a certain antineoplastic drug [3, 4]. In contrast to the increasing availability of data about genomics of drug sensitivity in vitro [5], the association with improved patient survival is so far limited to few clinical cases, e.g., exceptional responses to everolimus in bladder cancers with TSC1 mutations [6].
Here, we sought to systematically assess if the chances of overall survival in patients with a certain cancer type and treated with a given antineoplastic drug correlate with the presence of a certain genetic mutation in the tumor. The examined cohort comprised 3105 patients, spanning 12 cancer types (with 81–731 samples for each cancer type). Collectively, 21 antineoplastic drugs were administered each in at least 20 patients (median 82; IQR 29–150). Six cancer-associated mutations were detected in at least 20 patients in this cohort: V600E in BRAF (n = 29), R132H in IDH1 (n = 108), G12V in KRAS (n = 49), H1047R in PIK3CA (n = 89), R175H in TP53 (n = 45), and V777 deletion in ZFHX3 (n = 22). After binning samples by cancer type, out of 1512 potential associations, 9 associations between overall survival, drug treatment, and tumor mutation had sufficient sample size for each covariate and were hereby tested. The hazard ratio (HR) for each interaction between drug treatment and tumor mutation in a cancer type was estimated in a multivariate analysis using a nested Cox proportional hazard regression model. We adopted a likelihood ratio test to test whether there is a significant effect of the interaction on overall survival on top of the tumor mutation and administered drug alone (Additional file 1: Table S1).
We observed a significant effect only in one scenario, the interaction between temozolomide (TMZ) and R132H mutations in IDH1 on the overall survival of lower-grade glioma (LGG) (likelihood ratio test p = 0.026). This test suggests that the correlation with survival is specific to the interaction between TMZ and R132H mutations in IDH1 and not associated with the drug treatment or the mutation per se, as demonstrated by the Kaplan-Meier curves generated for patients stratified upon these features (log-rank test p = 0.047, Fig. 1). The median overall survival for patients with the interaction was 95 months (95 % CI, 63—N.E.) and for patients without the interaction was 62 months (95 % CI, 49–87).
We detected a significant prognostic value for the interaction using a univariate Cox proportional hazard regression model (p = 0.016, Table 1). However, the interaction violated the proportional hazard assumption and showed a time-dependent effect. The univariate analysis was also run on validated prognostic factors in LGG [7] and additional clinical features (Table 1). A multivariate analysis based on significant factors from the univariate analysis and including a time-dependent effect for the interaction revealed an independent positive correlation between the interaction and overall survival (HR 0.09, 95 % CI 0.01–0.58, p = 0.012), which tends to diminish over time (Additional file 1: Figure S1).
Table 1
Hazard ratio (HR) for clinical factors in the overall survival of lower-grade glioma
Factors
N [n death]
HR
Univariate
Multivariate
95 % CI
p
HR
95 % CI
p
Age
261
1.07
1.05–1.09
5e−10
1.07
1.05–1.09
6e−9
Gender
 
 Female
117 [30]
1
     
 Male
144 [33]
0.88
0.54–1.45
0.620
   
Temozolomide
 No
41 [24]
1
     
 Yes
220 [39]
0.80
0.47–1.35
0.398
   
R132H in IDH1
 Undetected
166 [45]
1
     
 Detected
95 [18]
0.74
0.43–1.29
0.292
   
Interaction drug-mutation
 Absent
181 [57]
1
  
1
  
 Present
80 [6]
0.35
0.15–0.83
0.016
0.09
0.01–0.58
0.012
Histology
 Astrocytoma
107 [27]
1
     
 Oligoastrocytoma/oligodendroglioma
154 [36]
0.67
0.40–1.10
0.112
   
Tumor grade
 Grade II
77 [18]
1
  
1
  
 Grade III
184 [45]
2.06
1.18–3.61
0.011
1.52
0.85–2.71
0.159
Laterality
 N.A.
1
      
 Left
131 [28]
1
     
 Midline
5 [1]
0.34
0.04–2.64
0.304
   
 Right
123 [33]
0.82
0.49–1.37
0.443
   
Tumor site
 N.A./other
3
   
1
  
 Supratentorial, frontal lobe
160 [36]
1
     
 Supratentorial, occipital lobe
5 [1]
0.71
0.10–5.20
0.736
   
 Supratentorial, parietal lobe
23 [4]
0.84
0.30–2.38
0.748
   
 Supratentorial, temporal lobe
70 [21]
1.86
1.08–3.22
0.026
1.22
0.70–2.11
0.481
Symptoms at diagnosis
 
 N.A./other
19
      
 Headaches
62 [18]
1
     
 Mental status changes
22 [8]
1.84
0.80–4.27
0.153
   
 Motor/movement changes
22 [6]
1.20
0.47–3.06
0.698
   
 Seizures
119 [23]
0.58
0.31–1.08
0.087
   
 Sensory changes
11 [2]
1.07
0.24–4.66
0.929
   
 Visual changes
6 [2]
0.69
0.16–2.98
0.617
   
In conclusion, we identified one genomic marker of drug sensitivity that was associated with better survival in patients, in contrast to patients treated with the same drug but with no detected mutation or vice versa. Indeed, mutations in IDH1 were previously implicated with good prognosis in brain tumors treated with TMZ [8, 9]. Our results independently validate these findings and further extend the reach of this correlation beyond some previous limitations [10]. First and foremost, the cohort size allowed discerning that an increase in patient survival was exquisitely associated with the interaction between IDH1 mutations and TMZ, suggestive of a synergy between treatment and tumor genomics. Second, it specifically correlated with R132H mutations. Finally, we recovered a negative time-dependent effect of the interaction, which is reminiscent of emergence of drug resistance and in line with the genetic evolution of lower-grade glioma attributed to TMZ treatment [11].

Acknowledgements

The authors acknowledge Knut and Alice Wallenberg Foundation for financing this work.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://​creativecommons.​org/​licenses/​by/​4.​0/​), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://​creativecommons.​org/​publicdomain/​zero/​1.​0/​) applies to the data made available in this article, unless otherwise stated.

Competing interests

The authors declare no conflict of interest in relation to the submitted work.

Authors’ contributions

FG conceived and designed the study and performed the analyses; JN supervised the study. All authors approved the manuscript in its final form.

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Metadaten
Titel
Systematic analysis of overall survival and interactions between tumor mutations and drug treatment
verfasst von
Francesco Gatto
Jens Nielsen
Publikationsdatum
01.12.2016
Verlag
BioMed Central
Erschienen in
Journal of Hematology & Oncology / Ausgabe 1/2016
Elektronische ISSN: 1756-8722
DOI
https://doi.org/10.1186/s13045-016-0249-2

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