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01.09.2009 | Original Article | Ausgabe 9/2009 Open Access

European Journal of Nuclear Medicine and Molecular Imaging 9/2009

Targeting murine heart and brain: visualisation conditions for multi-pinhole SPECT with 99mTc- and 123I-labelled probes

Zeitschrift:
European Journal of Nuclear Medicine and Molecular Imaging > Ausgabe 9/2009
Autoren:
M. Pissarek, J. Meyer-Kirchrath, T. Hohlfeld, S. Vollmar, A. M. Oros-Peusquens, U. Flögel, C. Jacoby, U. Krügel, N. Schramm
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1007/​s00259-009-1142-9) contains supplementary material, which is available to authorized users.

Abstract

Purpose

The study serves to optimise conditions for multi-pinhole SPECT small animal imaging of 123I- and 99mTc-labelled radiopharmaceuticals with different distributions in murine heart and brain and to investigate detection and dose range thresholds for verification of differences in tracer uptake.

Methods

A Triad 88/Trionix system with three 6-pinhole collimators was used for investigation of dose requirements for imaging of the dopamine D2 receptor ligand [123I]IBZM and the cerebral perfusion tracer [99mTc]HMPAO (1.2–0.4 MBq/g body weight) in healthy mice. The fatty acid [123I]IPPA (0.94 ± 0.05 MBq/g body weight) and the perfusion tracer [99mTc]sestamibi (3.8 ± 0.45 MBq/g body weight) were applied to cardiomyopathic mice overexpressing the prostaglandin EP3 receptor.

Results

In vivo imaging and in vitro data revealed 45 kBq total cerebral uptake and 201 kBq cardiac uptake as thresholds for visualisation of striatal [123I]IBZM and of cardiac [99mTc]sestamibi using 100 and 150 s acquisition time, respectively. Alterations of maximal cerebral uptake of [123I]IBZM by >20% (116 kBq) were verified with the prerequisite of 50% striatal of total uptake. The labelling with [99mTc]sestamibi revealed a 30% lower uptake in cardiomyopathic hearts compared to wild types. [123I]IPPA uptake could be visualised at activity doses of 0.8 MBq/g body weight.

Conclusion

Multi-pinhole SPECT enables detection of alterations of the cerebral uptake of 123I- and 99mTc-labelled tracers in an appropriate dose range in murine models targeting physiological processes in brain and heart. The thresholds of detection for differences in the tracer uptake determined under the conditions of our experiments well reflect distinctions in molar activity and uptake characteristics of the tracers.

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Zusatzmaterial
Supplement 1A Maximum intensity projection of the murine brain [123I]IBZM uptake in the brain after application of 0.61 MBq/g b.wt. Acquisition time 100 s, 10 steps of rotation, 8th acquisition period; salivary gland and thyroid in the upper part, striata in the centre. (GIF 268 kb)
259_2009_1142_MOESM1_ESM.gif
Supplement 1B Maximum intensity projection of the murine brain [99mTc]HMPAO uptake 75 to 98 min after application of 1.16 MBq/ g b. wt. Acquisition time 140 s, 10 steps of rotation. (GIF 547 kb)
259_2009_1142_MOESM2_ESM.gif
Supplement 2A Maximum intensity projection of murine hearts labelled with [ 99m Tc]sestamibi Cardiomyopathic heart 15-40 min after application of [99mTc]sestamibi (4.09 MBq/ g b.wt, ; 22 g b.wt.), liver in the upper part, heart in the centre. (GIF 361 kb)
259_2009_1142_MOESM3_ESM.gif
Supplement 2B Maximum intensity projection of murine hearts labelled with [ 99m Tc]sestamibi Wild-type heart (3.8 MBq/g b.wt.; 30 g b.wt.). Acquisition time 150 s, 10 steps of rotation. (GIF 338 kb)
259_2009_1142_MOESM4_ESM.gif
Literatur
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