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20.09.2016 | Original Article

Targeting Netrin-1 in glioblastoma stem-like cells inhibits growth, invasion, and angiogenesis

verfasst von: Tanwarat Sanvoranart, Aungkura Supokawej, Pakpoom Kheolamai, Yaowalak U-pratya, Niphon Poungvarin, Sith Sathornsumetee, Surapol Issaragrisil

Erschienen in: Tumor Biology | Ausgabe 11/2016

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Abstract

Glioblastoma (GBM) is an aggressive malignant brain tumor that still lacks effective therapy. Glioblastoma stem cells (GBM-SCs) were identified to contribute to aggressive phenotypes and poor clinical outcomes for GBM. Netrin-1, an axon guidance molecule, has been found in several tumors in adults. However, the role of Netrin-1 in GBM-SCs remains largely unknown. In this study, CD133-positive U251 GBM cells were used as a putative GBM-SC population to identify the functions of Netrin-1. Using lentiviral transduction, Netrin-1 miR RNAi vectors were transduced into CD133-positive U251 cells. We demonstrated that cell proliferation and survival were decreased following targeted deletion of Netrin-1. Cell invasion was dramatically diminished in Netrin-1 knockdown GBM-SCs. Moreover, Netrin-1 knockdown GBM-SCs exhibited less proangiogenic activity. In conclusion, Netrin-1 may represent a therapeutic target in glioblastoma.

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Titel: Targeting Netrin-1 in glioblastoma stem-like cells inhibits growth, invasion, and angiogenesis
verfasst von: Tanwarat Sanvoranart
Aungkura Supokawej
Pakpoom Kheolamai
Yaowalak U-pratya
Niphon Poungvarin
Sith Sathornsumetee
Surapol Issaragrisil
Publikationsdatum: 20.09.2016
Verlag: Springer Netherlands
Erschienen in: Tumor Biology / Ausgabe 11/2016
Print ISSN: 1010-4283
Elektronische ISSN: 1423-0380
DOI: https://doi.org/10.1007/s13277-016-5314-5

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Targeting Netrin-1 in glioblastoma stem-like cells inhibits growth, invasion, and angiogenesis

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