Introduction
Cancer cells have long been known to experience great metabolism alterations. Unlike normal cells which rely on mitochondrial oxidative phosphorylation, most cancer cells instead use aerobic glycolysis for ATP generation even in the presence of abundant oxygen supply, a phenomenon known as “the Warburg effect” (Warburg
1956). Elucidation of the metabolic differences and underlying mechanisms between cancer and normal cells could not only advance our understanding of fundamental cancer cell biology but also provide an important rationale for design of new therapeutic strategies aimed at selectively eliminating cancer cells by targeting their unique metabolism (Levine and Puzio-Kuter
2010). Over the past 30 years, increasing evidence has shown that glutamine (Gln) is an important metabolic substrate and energy source for many tumor cells which require Gln for their continued growth and survival, exhibiting so called “glutamine addiction” (Dang
2010; Wise and Thompson
2010). However, the complex metabolic logic of the proliferating cancer cells’ addiction to Gln, which goes far beyond satisfaction of energetic and biosynthetic needs, has only recently come into focus.
The solute-linked carrier family A1 member 5 (SLC1A5) gene encodes a Na
+-dependent neutral amino acid transporter, ASCT2 (Grewer and Grabsch
2004). ASCT2 is the major transporter responsible for Gln uptake into rapidly proliferating cells, including cancer cells (McGivan and Bungard
2007; Wise and Thompson
2010). Suppression of Gln uptake by ASCT2 pharmacological inhibitors or by shRNA-mediated knockdown of ASCT2 has been shown to successfully inhibit cancer cell growth and proliferation in a variety of tumor types including non-small cell lung cancer, prostate cancer, breast cancer, melanoma, and human head and neck squamous cell carcinoma (Cui et al.
2015; Gong et al.
2014; Hassanein et al.
2013,
2015; Lu et al.
2016; van Geldermalsen et al.
2016). More recently, anti-tumor efficacy of a novel anti-ASCT2 humanized monoclonal antibody, KM8094, has also been demonstrated in patient-derived xenograft (PDX) mouse models of gastric cancer (Kasai et al.
2017). Intriguingly, from this study, a correlation between anti-tumor efficacy and low antigen expression as well as low basal levels of glutamine uptake has been identified, suggesting that ASCT2 expression level could be a potential predictive biomarker for KM8094. In addition, metabolomics analysis revealed clear differences in intracellular energy status and redox status between responsive and non-responsive PDX models. The catabolism of Gln is mediated by two different subtypes of mitochondrial glutaminase (kidney or liver-type encoded by GLS or GLS2, respectively) to become glutamate (Glu), a versatile metabolic intermediate that connects with many distinct biological processes such as oncogenic signaling events (Curthoys and Watford
1995; Gao et al.
2009; Wang et al.
2010). In this regard, targeting glutaminase by a small molecule inhibitor has been demonstrated to inhibit oncogenic transformation (Wang et al.
2010). Tumors can utilize multiple sources of Gln including de novo synthesis of Gln from intracellular Glu through glutamine synthetase (GS, encoded by GLUL, glutamate–ammonia ligase) catalyzing the reverse reaction of the glutaminases to meet their requirements for Gln (Newsholme et al.
2003). While a positive correlation of GS activity with cell survival and proliferation has been observed in some types of cancers (Kung et al.
2011; Tardito et al.
2015; Yang et al.
2016), few studies have focused on GS as a potential target for Gln-based cancer therapy.
Gastric cancer (GC) represents the fourth most common malignant neoplasm and the second leading cause of cancer death (Yang
2006). Approximately 70% of GC cases occur in developing countries, especially in East Asia (Bott et al.
2015). Irrespective of the fact that most of GC patients present with an advanced disease at diagnosis, the key treatment issue is that GC has very limited sensitivity to current chemotherapy (Chen et al.
2016; Orditura et al.
2014). Effective systemic treatment for patients with GC is apparently an unmet need in medical oncology. Therefore, exploration of novel approaches to treat GC is urgently sought. Here, we proposed that both ASCT2 and GS that coordinately control Gln homeostasis may serve as potential new therapeutic targets for GC. We identified two subgroups of GC cells either sensitive or resistant to ASCT2 inhibitor BenSer and determined that their sensitivity to BenSer or Gln deprivation was largely dependent on the expression levels of GS and correlated with overall intracellular Gln content. In vivo studies revealed that combined therapy with ASCT2 and GS inhibitor was more efficacious against ASCT2- and GS-expressing GC xenograft tumor growth than the treatment of BenSer or MSO alone. We also found that ASCT2 and GS were differentially expressed in GC tissues with the majority of GS specimen expressing high-level ASCT2 but low GS. Thus, ASCT2 and GS might represent attractive companion biomarkers for selecting GC population most likely responding to Gln-targeted therapy and further validation of these potential targets for therapy targeting molecularly defined subtypes of GC is warranted.
Materials and methods
Cell culture and clinical samples
Six distinct differentiated human gastric cancer cell lines including HGC-27 (undifferentiated), NUGC-3 (poorly differentiated), MKN-45 (poorly differentiated), MGC-803 (poorly differentiated), AGS (moderately differentiated), and MKN-74 (moderately differentiated) were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). All cell lines were maintained in RPMI-1640 (Gibco BRL, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS) except AGS in Ham’s F12 medium (Cellgro, Manassas, VA, USA) and incubated at an atmosphere containing 5% CO2 at 37 °C. Human gastric cancer tissues and their paired adjacent normal mucosa tissues were collected from the First Affiliated Hospital of Fujian Medical University (Fuzhou, China) from 2008 to 2010. Samples were immediately stored in liquid nitrogen after surgical resection. All samples were collected with patients’ informed consent and the study was approved by the Institutional Review Board of the First Affiliated Hospital of Fujian Medical University.
Measurements of intracellular glutamine levels
Cells were plated into 24 well plates and grown to nearly 80% confluence. The extracellular medium was discarded and cells were quickly washed twice with HBSS (126.25-mM NaCl, 2-mM CaCl2, 3.0-M KCl, 1.25-mM NaH2PO4, 10-mM glucose, and 25-mM HEPES pH 7.4). Then, cells were incubated in 500 µl lysis buffer (1-mM DTT, 1-mM EDTA, and 10-mM NaOH) for 20 min and the lysis solution was harvested for consequent Gln measurement. Gln concentration was measured by a HPLC system as previously reported and was calculated from the amount of intracellular glutamine normalized to total amount of protein and expressed as nmol/mg protein.
RNA extraction and real-time quantitative PCR
Total RNA was extracted from cultured cells using Trizol reagent (Ambion, Carisbad, CA, USA) and RNA was reverse transcribed to cDNA using RT Reagent Kit (TaKaRa, Dalian, China). Then, real-time quantitative PCR of cDNA was prepared using SYBR Premix EX Taq kit (Takara, Shiga, Japan) and amplification was performed on Mx3000P QPCR system (Agilent Technology, Santa Clara, CA, USA). The respective forward and reverse primers were used to detect the relative expression levels of the target genes by the 2−△△ct method. The relative amount of target mRNA was normalized to β-actin. All the primers were designed by BioSune Biotechnology (Shang Hai) Co., Ltd.
Western blot analysis
Cells were lysed with Western and IP cell lysis buffer (Beyotime, Shanghai, China) containing PMSF (Amresco, Solon, Ohio, USA) on ice for 30 min, and centrifuged at 12,000g for 10 min at 4 °C to collect the supernatant. Cellular protein (40 µg per lane) was separated by 10% SDS-PAGE and transferred onto a 0.45-µM PVDF membrane (AmershamHybond, GE Healthcare, München, Germany). The membrane was blocked with 0.5% bovine serum album (Amresco, Solon, Ohio, USA) at room temperature for 2 h. Then, the membrane was incubated with rabbit anti-ASCT2 (1:1000; Abcam), rabbit anti-glutamine synthetase (1:1500; Abcam) and mouse anti-GAPDH (1:1500; Cell Signaling Technology) overnight at 4 °C. The membranes were washed three times with TBS-T (0.1% Tween-20) for 10 min each at room temperature, incubated in secondary antibody for 30 min at room temperature and detected using enhanced chemiluminescence substrate detection solution (Lulong biotech, Xiamen China).
Cell proliferation assay
Cells were seeded into 96-well plate at a density of 2 × 103 cells per well and cultured for 24 in Gln-supplemented or free medium. Cells were continuously exposed to ASCT2 competitive inhibitor benzylserine (BenSer) (Sigma-Aldrich, St Louis, MO, USA) and/or GS selective inhibitor l-methionine sulfoximine (MSO) (Sigma-Aldrich, St Louis, MO, USA). The proliferation of cells was evaluated by the Cell Counting Kit-8 (CCK-8, Dojindo, Kuma-moto, Japan). 10 µl CCK-8 reagent was added into each well and incubated for 4 h. The absorbance from each well was determined using a microplate reader at the wavelength of 450 nm (Bio-Tek, Winooski, VT, USA).
6 × 102 cells were grown in 60-mm plates containing complete growth medium and BenSer (10 mM) and/or L-MS (1 mM) for 14 days. For Gln-starvation experiments, the culture was replaced with Gln-free medium on day 7 and continued incubation for additional 7 days. Then, the colonies formed that contained 50 or more cells were counted after staining with crystal violet for 5 min.
Immunohistochemistry
Clinical specimens were dealt with dehydration of gradient ethanol and paraffin embedded, and processed into tissue sections with 4- µM thick for both tumor and paired adjacent normal gastric mucosa tissues. The sections were dewaxed in xylene and rehydrated in graded alcohol. Antigen retrieval was performed by 0.01-mol/L citrate buffer (pH 6.0) for 2 min. Endogenous peroxidase activity was inhibited with 3% hydrogen peroxide for 10 min. Sections were blocked by 5% BSA for 30 min at room temperature, and then incubated with rabbit anti-ACST2 (1:100; Abcam) and rabbit anti-Glutamine Synthetase (1:100; Abcam) at 4 °C overnight. The experimental procedure was performed according to the manufacturer’s instruction of the polink-2 plus Polymer HRP Detection System (ZSGB-bio, Beijing, China). Staining results were assessed by two pathologists independently.
Animal studies
All work performed with animals was in accordance with and approved by the Institutional Animal Care and Use Committee (IACUC) at the Fujian Medical University (Approval No. 2016-030). The in vivo antitumor efficacy of ASCT2 and GS inhibitors were assessed in 5–8-week-old male athymic BALB/c nude mice bearing HGC-27 tumor xenografts. 2 × 106 HGC-27 cells in 0.2 mL of RPMI 1640 medium were injected subcutaneously into the left and right posterior flank regions of each mouse. After the tumors were palpable, mice were randomly divided into four groups and the tumor volume was determined by the formula volume = length × width2/2. When the average tumor size in a group reached 100 mm3, the mice were treated with a single dose of vehicle control, BenSer (50 µg/kg), MSO (5 µg/kg) or the combination by the i.p. route. Then, the tumor size was measured every week for 4 weeks and plotted as a function of time to generate the in vivo growth curves. All animals were euthanized when the calculated tumor volume reached 1000 mm3 in either of the four groups.
Statistical analysis
Data are presented as mean ± SEM. All two-group comparisons used Student’s t test or paired t test and analyzed by IBM SPSS statistics version 19 for Windows (IBM Corp., USA). Figures were generated by GraphPad Prism 5 (GraphPad Software, Inc., USA). A two-tailed P value < 0.05 was defined to be statistically significant.
Discussion
Cancer cells have been well known for profound alterations in their metabolism, as exemplified by the Warburg effect, a phenomenon of cancer cells with elevated aerobic glycolysis (Kroemer and Pouyssegur
2008; Vander Heiden et al.
2009). Reprogramming of the cellular energy metabolism constitutes an emerging hallmark of cancer (Pavlova and Thompson
2016). Glutamine is a key metabolic substrate in cancer cells and is critical for tumor development, progression, and response to therapy (Lukey et al.
2013). Thus, elucidation of Gln metabolic differences would not only advance our understanding of fundamental cancer cell biology but also help characterize specific tumor groups to allow design of personalized therapies.
Gastric cancer is an aggressive tumor with low response to current standard of care cytostatic anticancer drugs. Therefore, modulation of the aberrant glutamine metabolism, if any, in GC may be a useful strategy for GC therapies. Since increased glutamine transporters account for glutamine addiction in most cancer cells we first attempted to test the sensitivity of six different gastric cancer cell lines to BenSer, a specific glutamine transport inhibitor. Interestingly, the sensitivity profile seemed to allow us to distinguish and possibly stratify the GC cells into two groups, one responsive to BenSer treatment and the other poorly responsive to the inhibitor. Unexpectedly, the sensitivity was not necessarily associated with ASCT2 expression levels but did correlate with intracellular Gln levels. This observation raised the question of whether glutamine dependence of GC cells for growth was also mediated by the cell-type-specific expression of glutamine synthetase (GS). To this end, the six GC cells were grown in Gln-free medium to deplete extracellular Gln supply, then the expression of GS and cell viability were determined in BenSer-sensitive and -resistant GC cells. We found that GS was barely expressed in BenSer-sensitive cells but abundant in BenSer-resistant cells, and that Gln deprivation led to the complete proliferative block in BenSer-sensitive cells without affecting the growth of BenSer-resistant cells. This finding suggests that GS was essential for the adaptation to the metabolic stress caused by Gln starvation and this adaptive importance of GS was consistent with the observation that exogenous Gln-deprived BenSer-resistant cells displayed an increased level of GS. The essential role of GS in determining cell viability under Gln deprivation was further supported by the fact that knockdown of GS in BenSer-resistant cells diminished cell proliferation, whereas forced expression of GS in BenSer-sensitive cells promoted growth.
A further question we asked was whether co-targeting of both ASCT2 and GS in the GC cells could produce a better therapeutic effect both in vitro and in vivo. On the availability of exogenous Gln that can be transported into the cell, GS-expressing BenSer-resistant cells were more sensitive to the combined treatment of ASCT2 and GS than used alone, indicating that both uptake of exogenous Gln and generation of endogenous Gln were limited as reflected by the significantly decreased levels of Gln inside the cells. However, for BenSer-sensitive cells with undetectable GS, GS inhibition by MSO did not further enhance the anti-proliferative of ASCT1 since the cells did not reply on endogenous synthesis of Gln for growth. Of important note, under Gln-deprived condition where BenSer-resistant cells fully depended on glutamine synthesized intracellularly by GS, MSO dramatically impaired cell proliferation. Furthermore, mouse xenograft model was employed to examine whether MSO and BenSer combination treatment could produce a higher efficacy against both ASCT2 and MS-expressing tumors. While BenSer produced a partial inhibition on tumor growth and MSO showed no treatment benefit, BenSer and MSO co-treatment significantly suppressed tumor growth. Collectively, these data support the possibility that such combination therapy may be an effective approach for treatment of GC patient population whose tumors express both ASCT2 and GS.
However, it should also be recognized that as energy metabolism and its regulatory machinery are evolutionarily conserved and shared by various normal cells, metabolism-related inhibitors such as BenSer and MSO may have a high probability of affecting the normal cells to produce adverse effects as well. In the history of targeting glutamine addiction, a number of pharmacologic agents that show promising results in preclinical studies have failed in clinical trials due to dose-limiting toxicities such as neurotoxicity, gastrointestinal toxicity, and myelosuppression (Ahluwalia et al.
1990). Therefore, whether the metabolic alterations in cancer cells can be targeted efficiently and safely in the clinic remains an unsolved issue. A more comprehensive understanding of the metabolic differences between cancer and normal cells through detail investigation might pave the way to achieve this goal. Furthermore, a combination of conventional chemotherapeutic agents and metabolic modulators may enhance therapeutic activity and should be further evaluated.
Immunohistochemistry of primary human GC samples demonstrated that ASCT2 was expressed to various extent in GC tissues of all 193 cases examined, which was in sharp contrast to adjacent normal gastric mucosa tissues negative for ASCT2 staining. ASCT2 is frequently upregulated in multiple cancers and inhibition of ASCT2-mediated glutamine uptake by pharmacological inhibitors of ASCT2 or by shRNA-mediated knockdown of ASCT2 has been shown to successfully inhibit cancer cell growth and proliferation (Cui et al.
2015; Gong et al.
2014; Hassanein et al.
2013,
2015; Lu et al.
2016; van Geldermalsen et al.
2016). Surprisingly, normal gastric tissues expressed a significantly higher level of GS than tumor tissues. Previous studies showed that multiple oncogenic signaling pathways have been found to increase the expression of GS and positive correlation of GS activity with cell survival and proliferation have been also observed in Myc-driven cancers (Bott et al.
2015; van der Vos et al.
2012; Yang et al.
2016). While ASCT2 immunohistochemical staining may be a useful pathological marker for GC as well, the biological significance of GS expression in gastric carcinogenesis and progression remains to be elucidated. It may be noteworthy that a correlation between ASCT2/GS expression and clinical outcome is not available in TCGA database. To investigate their prognostic significance, a large cohort including different types and stages of gastric cancers and associated with clinical data would be required. Regardless, from therapeutic point of view, selection of GC patients on the basis of ASCT2 and GS expression profile may prove to be a beneficial approach to target tumors for achieving desirable therapeutic outcomes.
In summary, this report demonstrates that Gln is a key driver of GC growth and Gln-targeting therapeutics may be of special clinical utility for gastric cancers with few current therapeutic options. Moreover, stratification of GC tumors based on ASCT2 and GS expression is important for personalized therapeutic strategies against the treated tumors by blocking glutamine transporters and/or inhibiting GS activity.