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Annals of Hematology

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01.03.2012 | Original Article

Tcf-1 gene silence suppresses downstream gene expression in CD4⁺ T cells from bone marrow of aplastic anemia patients

verfasst von: Miao Zheng, Kai Zheng, Jianfeng Zhou, Hanying Sun, Lifang Huang, Wenli Liu

Erschienen in: Annals of Hematology | Ausgabe 3/2012

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Abstract

CD4⁺ T cells play a crucial role in the pathogenesis of acquired aplastic anemia (AA). Tcf-1 gene regulates T cell development and function, and it is significantly upregulated in the bone marrow CD4⁺ T cells from patients with acquired AA. To explore the role of Tcf-1 in the pathogenesis of AA, we knocked down Tcf-1 gene in CD4⁺ T cells of AA patients and studied the effects of Tcf-1 silencing on its downstream gene expression. Upon transfection of psiRNA into marrow CD4⁺ T cells from bone marrow of aplastic anemia patients, the expression of Tcf-1 was significantly knocked down; consequently, expressions of c-Myc and CD44 were also significantly reduced. Our results suggest that Tcf-1 may contribute to pathogenesis of AA by regulating downstream gene expression such as c-myc and CD44.

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Titel: Tcf-1 gene silence suppresses downstream gene expression in CD4+ T cells from bone marrow of aplastic anemia patients
verfasst von: Miao Zheng
Kai Zheng
Jianfeng Zhou
Hanying Sun
Lifang Huang
Wenli Liu
Publikationsdatum: 01.03.2012
Verlag: Springer-Verlag
Erschienen in: Annals of Hematology / Ausgabe 3/2012
Print ISSN: 0939-5555
Elektronische ISSN: 1432-0584
DOI: https://doi.org/10.1007/s00277-011-1314-0

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Tcf-1 gene silence suppresses downstream gene expression in CD4⁺ T cells from bone marrow of aplastic anemia patients

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