Temperature and depth evaluation of the in vitro effects of femtosecond laser on oral soft tissue, with or without air-cooling

Femtosecond laser is an effective and safe tool in many surgeries, but the studies of its effect on oral soft tissue ablation are insufficient. This study aimed to investigate the effect of soft tissue ablation with a 1030-nm femtosecond laser on temperature and depth. Twenty Sprague–Dawley rat tongue specimens were obtained and flat-mounted. The 1030-nm femtosecond laser was controlled by a computer system, with a set distance of 4.7 mm between the laser aperture and soft tissue surfaces. Ten specimens were ablated for > 1 min with or without air-cooling for temperature measurement, while the other 10 specimens were ablated for depth measurements, using the following parameters: (i) 3 W, 2000 mm/s; (ii) 3 W, 4000 mm/s; (iii) 5 W, 2000 mm/s; (iv) 5 W, 4000 mm/s; (v) 8 W, 2000 mm/s; (vi) 8 W, 4000 mm/s. Temperature changes were measured using a type-K thermocouple. The depth attained using different power and scanning speed settings was measured by a three-dimensional morphology measurement laser microscope. Laser power, scanning speed, and air-cooling effects were determined. Higher energy and lower speed induced higher temperatures (p < 0.05), which were significantly decreased by air-cooling (p < 0.05). The lowest ablation depth was obtained at 3 W and 4000 mm/s (72.63 ± 6.47 μm) (p < 0.05). The greatest incision depth was achieved at 8 W and 2000 mm/s (696.19 ± 35.37 μm), or 4000 mm/s (681.16 ± 55.65 μm) (p < 0.05). The 1030-nm femtosecond laser application demonstrates clinically acceptable ablation efficiency, without marked temperature damage, in a controlled manner.