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Erschienen in: Intensive Care Medicine 8/2012

01.08.2012 | Experimental

Temperature- and time-dependent changes in TLR2-activated microglial NF-κB activity and concentrations of inflammatory and anti-inflammatory factors

verfasst von: Tomohiro Matsui, Moe Tasaki, Takahiro Yoshioka, Yukari Motoki, Hidehiro Tsuneoka, Junzo Nojima

Erschienen in: Intensive Care Medicine | Ausgabe 8/2012

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Abstract

Purpose

Therapeutic hypothermia protects neurons following injury to the central nervous system (CNS). Microglia express toll-like receptors (TLRs) that play significant roles in pathological processes in sterile CNS injury. We have examined the effects of culture temperature on the TLR2-activated microglial production of cytokines and nitric oxide (NO), which are known to be associated with CNS damage, and the possible involvement of nuclear factor-κB (NF-κB) activation underlying such effects.

Methods

Rat microglia were cultured with a selective TLR2 agonist, Pam3CSK4, under hypothermic, normothermic, and hyperthermic conditions, and with Pam3CSK4 in the presence of a NF-κB activation inhibitor at 37 °C. Cytokine and NO levels and NF-κB p65 activation were measured.

Results

The production of tumor necrosis factor-alpha (TNF-α), interleukin-10 (IL-10), and NO and the activation of NF-κB p65 were reduced by hypothermia, but augmented by hyperthermia at 3–6, 24–48, 48, and 0.5 h, post-treatment initiation, respectively. Pharmacological inhibition of NF-κB activation impaired the Pam3CSK4-induced TNF-α, IL-10, and NO production.

Conclusions

In TLR2-activated microglia, hypothermia reduced, while hyperthermia increased, the early activation of NF-κB and the subsequent NF-κB-mediated production of TNF-α, IL-10, and NO in a time-dependent manner, suggesting that attenuation of these factors via suppression of NF-κB in microglia is one possible neuroprotective mechanism of therapeutic hypothermia. Moreover, temperature-dependent changes in microglial TNF-α production during the early phase and IL-10 and NO production during the late phase indicate that these factors might be useful as clinical markers to monitor hypothermia-related neuronal protection and hyperthermia-related neuronal injury.
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Metadaten
Titel
Temperature- and time-dependent changes in TLR2-activated microglial NF-κB activity and concentrations of inflammatory and anti-inflammatory factors
verfasst von
Tomohiro Matsui
Moe Tasaki
Takahiro Yoshioka
Yukari Motoki
Hidehiro Tsuneoka
Junzo Nojima
Publikationsdatum
01.08.2012
Verlag
Springer-Verlag
Erschienen in
Intensive Care Medicine / Ausgabe 8/2012
Print ISSN: 0342-4642
Elektronische ISSN: 1432-1238
DOI
https://doi.org/10.1007/s00134-012-2591-3

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