Fig. 1
Western blot analysis of cartilage extracts from patients using an anti-TN-C antibody. A total of 1 μg protein was run per well and membranes developed with alkaline phosphatase (AP) substrate for 1 min each. Sample identification: 1 29 (F), lower leg, amputation; 2 17 (F), lower limb, osteosarcoma; 3 45 (M), lower limb, amputation; 4 72 (M), hip OA; 5 68 (M), hip OA; 6 72 (M), knee OA; 7 75 (F), knee OA; 8 71 (M), hip OA; 9 70 (F), knee OA; 10 66 (F), knee OA; 11 45 (M), hip, RA; 12 69 (F), hip, RA. b Equivalent samples used as in (a) with 1 μg protein per well stained with Coomassie Brilliant Blue