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Clinical and Translational Oncology

06.09.2024 | RESEARCH ARTICLE

TET3 is expressed in prostate cancer tumor-associated macrophages and is associated with anti-androgen resistance

verfasst von: Qiu-ju Wei, Hai-qi Liang, Yao-wen Liang, Zu-xin Huang

Erschienen in: Clinical and Translational Oncology

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Abstract

Purpose

The aim of this study is to investigate the expression of TET3 in prostate cancer and its effect on the efficacy of anti-androgen therapy (ADT).

Methods

The expression of TET3 in 1965 cases of prostate cancer and 493 cases of normal prostate tissues were analyzed. The CIBERSORT algorithm evaluated the abundance of 22 tumor-infiltrating immune cells in 497 prostate cancers. Subsequently, the expression of TET3 in prostate cancer TAMs was analyzed using 21,292 cells from single-cell RNA sequencing (scRNAseq). In addition, the trajectory of the differentiation process was reconstructed based on pseudotime analysis. Sensitivity prediction of prostate cancers to ADT was evaluated based on GDSC2 and CTRP databases. Another dataset GSE111177 was employed for further analysis.

Results

TET3 was over-expressed in prostate cancer, and the expression of TET3 in metastatic prostate cancer was higher than that in non-metastatic prostate cancer. The scRNAseq analysis of prostate cancer showed that TET3 was mainly expressed in TAM. TET3 expressed in early and active TAMs, with the activation of signaling pathways such as energy metabolism, cell communication, and cytokine production. Prostate cancer in TET3 high expression group was more sensitive to ADT drugs such as Bicalutamide and AZD3514, and was also more sensitive to chemotherapy drugs such as Cyclophosphamide, Paclitaxel, and Vincristine, and MAPK pathway inhibitors of Docetaxel and Dabrafenib.

Conclusions

The efficacy of ADT in prostate cancer is related to the expression of TET3 in TAMs, and TET3 may be a potential therapeutic target for coordinating ADT.
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Metadaten
Titel
TET3 is expressed in prostate cancer tumor-associated macrophages and is associated with anti-androgen resistance
verfasst von
Qiu-ju Wei
Hai-qi Liang
Yao-wen Liang
Zu-xin Huang
Publikationsdatum
06.09.2024
Verlag
Springer International Publishing
Erschienen in
Clinical and Translational Oncology
Print ISSN: 1699-048X
Elektronische ISSN: 1699-3055
DOI
https://doi.org/10.1007/s12094-024-03708-w

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