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04.08.2016 | Original Paper | Ausgabe 1/2017

Journal of Natural Medicines 1/2017

The action and mechanism of myrislignan on A549 cells in vitro and in vivo

Zeitschrift:
Journal of Natural Medicines > Ausgabe 1/2017
Autoren:
XinGang Lu, Liu Yang, JingXian Chen, JiAn Zhou, XiaoDan Tang, YingGang Zhu, HongFu Qiu, Jie Shen
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1007/​s11418-016-1029-6) contains supplementary material, which is available to authorized users.
X. Lu and L. Yang contributed to this work equally.

Abstract

Myrislignan is a natural compound with little pharmacological study. In our investigation, we investigated the effect of myrislignan in the induction of apoptosis in A549 cells in vitro and in vivo. Myrislignan inhibited the proliferation of A549 cells in a dose- and time-dependent manner assayed by MTT. In addition, Hoechst flow cytometry showed that myrislignan significantly induced apoptosis and cell cycle arrest in A549 cells. The apoptosis and anti-cell proliferation was mediated by the activation of mitogen-activated protein kinase and the inhibition of epidermal growth factor receptor signal pathway, change of mitochondrial membrane potential, the releasing of c-Myc, the downregulation of the level of the anti-apoptotic protein Bcl-2, and the upregulation of the level of the pro-apoptotic protein Bax. In conclusion, those results reveal a potential mechanism for the anti-cancer effect of myrislignan on human lung cancer, while suggesting that myrislignan may be a promising compound for the treatment of lung cancer.

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Zusatzmaterial
Supplementary Fig. 1. Myrislignan induced apoptosis and cell cycle arrest in A549 cells. Flow cytometry assay was used to determine the apoptosis of A549 cells with increasing concentrations of myrislignan or combined with EGF (100 ng/ml) for 48 h. a Annexin-V/PI assay. b Cell cycle assay. Statistical differences were considered significant at the levels of *p < 0.05, **p < 0.01, or ***p < 0.001
11418_2016_1029_MOESM1_ESM.tif
Literatur
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