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Pathology & Oncology Research

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01.04.2015 | Research

The Apoptotic Effect of Plant Based Nanosilver in Colon Cancer Cells is a p53 Dependent Process Involving ROS and JNK Cascade

verfasst von: Shakti Ranjan Satapathy, Purusottam Mohapatra, Dipon Das, Sumit Siddharth, Chanakya Nath Kundu

Erschienen in: Pathology & Oncology Research | Ausgabe 2/2015

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Abstract

Here, we report the p53 dependent mitochondria-mediated apoptotic mechanism of plant derived silver-nanoparticle (PD-AgNPs) in colorectal cancer cells (CRCs). PD-AgNPs was synthesized by reduction of AgNO₃ with leaf extract of a medicinal plant periwinkle and characterized. Uptake of PD-AgNPs (ξ - 2.52 ± 4.31 mV) in HCT116 cells was 3 fold higher in comparison to synthetic AgNPs (ξ +2.293 ± 5.1 mV). A dose dependent increase in ROS production, activated JNK and decreased mitochondrial membrane potential (MMP) were noted in HCT116 but not in HCT116 p53 ^−/− cells after PD-AgNP exposure. PD-AgNP-mediated apoptosis in CRCs is a p53 dependent process involving ROS and JNK cascade.

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Satapathy SR, Mohapatra PM, Preet R, Das D, Sarkar B, Choudhuri T, Wyatt MD, Kundu CN (2013) Silver nanoparticles induce apoptosis in human colon cancer cells mediated through p53. Nanomedicine 8:1307–1322. doi:10.2217/nnm.12.176 CrossRefPubMed

Tao A, Sinsermsuksaku P, Yang P (2006) Polyhedral silver nanocrystals with distinct scattering signatures. Angew Chem Int Ed 45:4561–4597. doi:10.1002/anie.200601277 CrossRef

AshaRani PV, Low Kah Mun G, Hande MP, Valiyaveettil S (2009) Cytotoxicity and genotoxicity of silver nanoparticles in human cells. ACS Nano 3:279–290. doi:10.1021/nn800596w CrossRefPubMed

Raveendran P, Fu J, Wallen SL (2003) Completely “green” synthesis and stabilization of metal nanoparticles. J Am Chem Soc 125:13940–13951. doi:10.1021/ja029267j CrossRefPubMed

Jha AK, Prasad K, Kulkarni AR (2009) Synthesis of TiO₂ nanoparticles using microorganisms. Colloids Surf B: Biointerfaces 71:226–229. doi:10.1016/j.colsurfb.2009.02.007 CrossRefPubMed

Dhillon GS, Brar SK, Kaur S, Verma M (2012) Green approach for nanoparticle biosynthesis by fungi: current trends and applications. Crit Rev Biotechnol 32:49–73. doi:10.3109/07388551.2010.550568 CrossRefPubMed

Iravani S (2011) Green synthesis of metal nanoparticles using plants. Green Chem 13:2638–2650. doi:10.1039/C1GC15386B CrossRef

Mukunthan K, Balaji S (2012) Cashew apple juice (Anacardium occidentale L.) speeds up the synthesis of silver nanoparticles. Int J Green Nanotechnol 4:71–79. doi:10.1080/19430892.2012.676900 CrossRef

Kasthuri J, Veerapandian S, Rajendran N (2009) Biological synthesis of silver and gold nanoparticles using apiin as reducing agent. Colloids Surf B: Biointerfaces 68:55–60. doi:10.1016/j,colsurfb.2008.09.021 CrossRefPubMed

10.

Goyal P (2008) In vitro evaluation of crude extracts of Catharanthus roseus for potential antibacterial activity. Int J Green Pharm 2:176–181. doi:10.4103/0973-8258.42739 CrossRef

11.

Rasineni K, Bellamkonda R, Singareddy SR, Desireddy S (2010) Antihyperglycemic activity of Catharanthus roseus leaf powder in streptozotocin-induced diabetic rats. Pharmacogn Res 2:195–201. doi:10.4103/0974-8490.65523 CrossRef

12.

Ponarulselvam S, Panneerselvam C, Murugan K, Aarthi N, Kalimuthu K, Thangamani S (2012) Synthesis of silver nanoparticles using leaves of Catharanthus roseus Linn. G. Don and their antiplasmodial activities. Asian Pac J Trop Biomed 2:574–580. doi:10.1016/S2221-1691(12)60100-2 CrossRefPubMedCentralPubMed

13.

Jacob SJ, Finub JS, Narayanan A (2012) Synthesis of silver nanoparticles using Piper longum leaf extracts and its cytotoxic activity against Hep-2 cell line. Colloids Surf B: Biointerfaces 91:212–214. doi:10.1016/j.colsurfb.2011.11.001 CrossRefPubMed

14.

Suzuki H, Toyooka T, Ibuki Y (2007) Simple and easy method to evaluate uptake potential of nanoparticles in mammalian cells using a flow cytometric light scatter analysis. Environ Sci Technol 41:3018–24. doi:10.1021/es0625632 CrossRefPubMed

15.

Parang Z, Keshavarz A, Farahi S, Elahi SM, Ghoranneviss M, Parhoodeh S (2012) Fluorescence emission spectra of silver and silver/cobalt nanoparticles. Scientia Iranica F 19:943–47. doi:10.1016/j.scient.2012.02.026 CrossRef

16.

Chairuangkitti P, Lawanprasert S, Roytrakul S, Aueviriyavit S, Phummiratch D, Kulthong K, Chanvorachote P, Maniratanachote R (2013) Silver nanoparticles induce toxicity in A549 cells via ROS-dependent and ROS-independent pathways. Toxicol Vitro 27:330–338. doi:10.1016/j.tiv.2012.08.021 CrossRef

17.

Kumar S, Sitasawad SL (2009) N-acetylcysteine prevents glucose/glucose oxidase-induced oxidative stress, mitochondrial damage and apoptosis in h9c2 cells. Life Sci 84:328–36. doi:10.1002/jctb.2023 CrossRefPubMed

18.

Wu GS (2004) The functional interactions between the p53 and MAPK signaling pathways. Cancer Biol Ther 3:156–161. doi:10.4161/cbt.3.2.614 CrossRefPubMed

19.

Saha MN, Jiang H, Yang Y, Zhu X, Wang X, Schimmer AD, Qiu L, Chang H (2012) Targeting p53 via JNK Pathway: a novel role of RITA for apoptotic signaling in multiple myeloma. PLoS ONE 7:e30215. doi:10.1371/journal.pone.0030215 CrossRefPubMedCentralPubMed

20.

Taylor CA, Zheng Q, Liu Z, Thompson JE (2013) Role of p38 and JNK MAPK signaling pathways and tumor suppressor p53 on induction of apoptosis in response to AdeIF5A1 in A549 lung cancer cells. Mol Cancer 12:35–45. doi:10.1186/1476-4598-12-35 CrossRefPubMedCentralPubMed

21.

Wilhelm C, Billotey C, Roger J, Pons JN, Bacri JC, Gazeau F (2003) Intracellular uptake of anionic superparamagnetic nanoparticles as a function of their surface coating. Biomaterials 24:1001–1011. doi:10.1016/S0142-9612(02)00440-4 CrossRefPubMed

22.

Patil S, Sandberg A, Heckert E, Self W, Seal S (2007) Protein adsorption and cellular uptake of cerium oxide nanoparticles as a function of zeta potential. Biomaterials 28:4600–4607. doi:10.1016/j.biomaterials.2007.07.029 CrossRefPubMedCentralPubMed

23.

Lim HK, Asharani PV, Hande MP (2012) Enhanced genotoxicity of silver nanoparticles in DNA repair deficient Mammalian cells. Front Genet 3:104–116. doi:10.3389/fgene.2012.00104 CrossRefPubMedCentralPubMed

24.

Derfus AM, Chan WCW, Bhatia SN (2004) Intracellular delivery of quantum dots for live cell labeling and organelle tracking. Adv Mater 16:961–966. doi:10.1021/nl102172j CrossRef

25.

Xia T, Kovochich M, Brant J, Hotze M, Sempf J, Oberley T, Sioutas C, Yeh JI, Wiesner MR, Nel AE (2006) Comparison of the abilities of ambient and manufactured nanoparticles to induce cellular toxicity according to an oxidative stress paradigm. Nano Lett 6:1794–1807. doi:10.1021/nl061025k CrossRefPubMed

26.

Zhivotovsky B, Orrenius S, Brustugus OT, Doskeland SO (1998) Injected cytochrome c induces apoptosis. Nature 391:449–50. doi:10.1038/35060 CrossRefPubMed

27.

Johnson BW, Cepero E, Boise LH (2000) Bcl-xL inhibits cytochrome c release but not mitochondrial depolarization during the activation of multiple death pathways by tumor necrosis factor-alpha. J Biol Chem 275:31546–31553. doi:10.1074/jbc.M001363200 CrossRefPubMed

28.

Piao MJ, Kang KA, Lee IK, Kim HS, Kim S, Choi JY, Choi J, Hyun JW (2011) Silver nanoparticles induce oxidative cell damage in human liver cells through inhibition of reduced glutathione and induction of mitochondria-involved apoptosis. Toxicol Lett 201:92–100. doi:10.1016/j.toxlet.2010.12.010 CrossRefPubMed

29.

Cargnello M, Roux PP (2011) Activation and function of the MAPKs and their substrates, the MAPK-activated protein kinases. Microbiol Mol Biol Rev 75:50–83. doi:10.1128/MMBR.00031-10 CrossRefPubMedCentralPubMed

30.

Hsin YH, Chen CF, Huang S, Shih TS, Lai PS, Chueh PJ (2008) The apoptotic effect of nanosilver is mediated by a ROS and JNK dependent mechanism involving the mitochondrial pathway in NIH3T3 cells. Toxicol Lett 179:130–139. doi:10.1016/j.toxlet.2008.04.015 CrossRefPubMed

Titel: The Apoptotic Effect of Plant Based Nanosilver in Colon Cancer Cells is a p53 Dependent Process Involving ROS and JNK Cascade
verfasst von: Shakti Ranjan Satapathy
Purusottam Mohapatra
Dipon Das
Sumit Siddharth
Chanakya Nath Kundu
Publikationsdatum: 01.04.2015
Verlag: Springer Netherlands
Erschienen in: Pathology & Oncology Research / Ausgabe 2/2015
Print ISSN: 1219-4956
Elektronische ISSN: 1532-2807
DOI: https://doi.org/10.1007/s12253-014-9835-1

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The Apoptotic Effect of Plant Based Nanosilver in Colon Cancer Cells is a p53 Dependent Process Involving ROS and JNK Cascade

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