Background
Methods
The goal
Search methods for the identification of studies
Criteria for the consideration of studies for this review
Data extraction and management
Assessment of the risk of bias for animal studies
Results
Search process
Included studies
The cardioprotective effects of NC
Study | Species | Weight(g) | Random | Groups | Outcome measure |
---|---|---|---|---|---|
Tao Yang et al. 2012 | SD rats | 200 ± 20 | Not mentioned | The sham group (n = 10) The I/R model group (n = 10) The volatile oil of NC group-1 (n = 10) The volatile oil of NC group-2 (n = 10) | IL-6, TNF-ɑ, CK-MB, cTNT, Infarct size, The frequency and time of cardiac arrhythmia |
Rajakannu Subashini et al. 2006 | Wistar rats | 120–130 | Not mentioned | The control group (n = 6) The doxorubicin group (n = 6) The extract of NC group (n = 6) The doxorubicin + extract of NC group (n = 6) | Body weight; Heart weight; LDH; CPK; AST; ALT; SOD; CAT; GPx; GST; LPO |
The antiarrhythmic effect of NC
Study | Cell | Intervention | Groups | Target | Outcome measure |
---|---|---|---|---|---|
Xiangyu Li et al. 2013 | The normal isolated ventricular myocytes of rats | The volatile oil of NC | The volatile oil of NC group-1 (1 μg/g) The volatile oil of NC group-2 (3 μg/g) The volatile oil of NC group-3 (5 μg/g) The volatile oil of NC group-4 (10 μg/g) The volatile oil of NC group-5 (20 μg/g) The volatile oil of NC group-6 (50 μg/g) | Ik; Ik1
| Dose-response; I-V curve; Activation curve; Inactivation curve |
Qizhu Tang et al. 2004 | The normal isolated ventricular myocytes of rabbits | The extract of NC | The control group The extract of NC group-1 (5 g/L) The extract of NC group-2 (10 g/L) | INa; ICa-L
| Dose-response; I-V curves |
Ming Cao et al. 2010 | The normal isolated ventricular myocytes of rats | The volatile oil of NC | The volatile oil of NC group-1 (3 μg/g) The volatile oil of NC group-2 (5 μg/g) The volatile oil of NC group-3 (10 μg/g) The volatile oil of NC group-4 (20 μg/g) The volatile oil of NC group-5 (50 μg/g) | ICa-L
| Dose-response; I-V curve; Activation curve; Inactivation curve |
Yuanwei Liu et al. 2009 | The normal isolated ventricular myocytes of rats | The extract of NC | The control group The extract of NC group-2 (10 g/L) | INa; Ito
| I-V curve |
Tao Yang et al. 2009 | The normal isolated ventricular myocytes of rats | The volatile oil of NC | The volatile oil of NC group-1 (1 μg/g) The volatile oil of NC group-2 (3 μg/g) The volatile oil of NC group-3 (5 μg/g) The volatile oil of NC group-4 (10 μg/g) The volatile oil of NC group-5 (100 μg/g) | INa
| Dose-response; I-V curve; Activation curve; Inactivation curve |
Langjie Hu et al. 2009 | The normal isolated ventricular myocytes of rats | The volatile oil of NC | The volatile oil of NC group-1 (3 μg/g) The volatile oil of NC group-2 (6 μg/g) The volatile oil of NC group-3 (10 μg/g) The volatile oil of NC group-4 (20 μg/g) | Ito
| Dose-response; I-V curve; Activation curve; Inactivation curve |
Yuzhi Ge et al. 2009 | Nav 1.5-HEK cell | The volatile oil of NC | The volatile oil of NC group-1 (3 ppm) The volatile oil of NC group-2 (10 ppm) | INa
| Dose-response; Voltage-dependent curve |
Zhiting Wu et al. 2009 | Nav 1.5-HEK cell | The volatile oil of NC | The volatile oil of NC group (10 ppm) | INa
| I-V curve; G-V curve |
Yanyang Liu et al. 2013 | Nav 1.5-HEK cell | The volatile oil of NC | The volatile oil of NC group (5 ppm) | INa
| Frequency-dependent curve |
Study | Xiangyu Li et al. 2013 | Xiangyu Li et al. 2013 | Ming Cao et al. 2010 | Tao Yang et al. 2009 | Langjie Hu et al. 2009 |
---|---|---|---|---|---|
Type | Ik
| Ik1
| ICa-L
| INa
| Ito
|
Inhib-ition Ratio | 1 μg/g (10.45 ± 1.14)% 3 μg/g (20.18 ± 2.54)% 5 μg/g (46.88 ± 5.30)% 10 μg/g (78.00 ± 9.20)% 20 μg/g (96.00 ± 6.40)% 50 μg/g (98.00 ± 5.60)% | 1 μg/g (5.78 ± 1.32)% 3 μg/g (12.45 ± 1.14)% 5 μg/g (29.18 ± 2.44)% 10 μg/g (52.43 ± 6.3)% 20 μg/g(89.74 ± 11.3)% 50 μg/g (98.96 ± 4.6)% | 3 μg/g (11.18 ± 1.48)% 5 μg/g (24.83 ± 4.82)% 10 μg/g (45.72 ± 3.54)% 20 μg/g (76.87 ± 7.03)% 50 μg/g (100 ± 0)% | 1 μg/g (7.2 ± 4)% 3 μg/g (20 ± 3.7)% 5 μg/g (55 ± 5)% 10 μg/g (76 ± 3.5)% 100 μg/g (89 ± 3)% | 3 μg/g (27.01 ± 6.93)% 6 μg/g (51.13 ± 9.82)% 10 μg/g (80.86 ± 4.63)% 20 μg/g (94.81 ± 4.30)% |
Study | Type | Dose | Number | Half of the activating voltage (mV) | Slope factor | ||
---|---|---|---|---|---|---|---|
Pre-treatment | Post-treatment | Pre-treatment | Post-treatment | ||||
Xiangyu Li et al. 2013 | Ik
| 5 μg/g | 6 | 23.65 ± 0.65 | 28.19 ± 0.57* | 6.09 ± 0.56 | 5.14 ± 0.51* |
Ming Cao et al. 2010 | ICa-L
| 10 μg/g | 5 | −5.47 ± 0.50 | −2.77 ± 0.49* | 4.68 ± 0.39 | 4.50 ± 0.40 |
Tao Yang et al. 2009 | INa
| 5 μg/g | 6 | −43.65 ± 0.98 | −40.25 ± 1.01* | 5.63 ± 0.75 | 5.03 ± 0.80* |
Langjie Hu et al. 2009 | Ito
| 6 μg/g | 5 | 36.06 ± 1.79 | 34.79 ± 3.03 | 22.97 ± 1.49 | 30.79 ± 2.90 |
Study | Type | Dose | Number | Half of the activating voltage (mV) | Slope factor | ||
---|---|---|---|---|---|---|---|
Pre-treatment | Post-treatment | Pre-treatment | Post-treatment | ||||
Xiangyu Li et al. 2013 | Ik
| 5 μg/g | 6 | −64.46 ± 1.02 | −82.84 ± 1.27* | 14.40 ± 1.13 | 13.35 ± 1.06* |
Ming Cao et al. 2010 | ICa-L
| 10 μg/g | 5 | −20.82 ± 0.48 | −29.44 ± 1.03* | 6.16 ± 0.43 | 11.05 ± 0.86* |
Tao Yang et al. 2009 | INa
| 5 μg/g | 6 | −100.92 ± 0.68 | −111.20 ± 0.86* | 10.33 ± 0.62 | 11.33 ± 0.74* |
Langjie Hu et al. 2009 | Ito
| 6 μg/g | 5 | −33.74 ± 0.48 | −40.54 ± 0.70* | 5.00 ± 0.40 | 8.42 ± 0.62* |
Study | Species | Weight(g) | Random | Groups | Outcome measure |
---|---|---|---|---|---|
Peng Jian et al. 2015 | Wistar rats | 200 ± 20 | Random digits table | The normal group (n = 20) The tachyarrhythmia group (n = 20) The Nardosinone group (n = 20) | HR; Heart coefficient; LVSP; LVEDP; +dp/dtmax; −dp /dtmax; cAMP; PKA |
Jing Zhang et al. 2014 | SD rats | 280–310 | Not mentioned | The control group (n = 24) The metoprolol group (n = 24) The NC group (n = 24) | PVCs; VTs; VFs; HR; MAP; P-R interval; QRS interval; Q-T interval; Cx43; Infarct size of the left ventricular |
Ping Zhou et al. 2007 | Wistar rats | 250 ± 50 | Not mentioned | The control group-1 (n = 6) The control group-2 (n = 6) The volatile oil of NC group-1 (n = 20) The volatile oil of NC group-2 (n = 20) | ERP; ERP/RR |
Assessment of the risk of bias for animal studies
Studies | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
---|---|---|---|---|---|---|---|---|---|---|
Tao Yang et al. 2012 | Unclear | Yes | Unclear | Unclear | Unclear | Unclear | Unclear | Yes | Unclear | Unclear |
Rajakannu Subashini et al. 2006 | Unclear | Yes | Unclear | Unclear | Unclear | Unclear | Unclear | Yes | Unclear | Unclear |
Peng Jian et al. 2015 | Yes | Yes | Unclear | Unclear | Unclear | Unclear | Unclear | Yes | Unclear | Unclear |
Jing Zhang et al. 2014 | Unclear | Yes | Unclear | Unclear | Unclear | Unclear | Unclear | Yes | Unclear | Unclear |
Ping Zhou et al. 2007 | Unclear | Yes | Unclear | Unclear | Unclear | Unclear | Unclear | Yes | Unclear | Unclear |