According to the World Health Organization (WHO) definition, infertility is a disability in pregnancy after one year of normal sexual intercourse and without prevention [
1]. It is estimated that about 50 to 80 million people around the world experience infertility during their reproductive life [
2]. Based on a systematic analysis of 277 demographic and reproductive health surveys, in 2010, among women 20 to 44 years old who were exposed to the risk of pregnancy, 1.9% (95% CI: 1.7 to 2.2%) were unable to achieve a live birth. Also among women who had a living child and were exposed to the risk of pregnancy, 10.5% (95% CI: 9.5 to 11.7%) were unable to have another child. In parallel with the population growth, the absolute number of infertile couples has also increased. The number of infertile couples increased from 42.0 million (95% CI: 39.6 million to 44.8 million) in 1990 to 48.5 million (95% CI: 45.0 million to 52.6 million) in 2010. It is expected to keep growing, and estimates have put the total infertile couple at 60 million by mid-2030 [
3]. Over the past three decades, many cases of infertility have resulted in pregnancy through the modern fertility treatments. There are several types of ARTs, that are used depend on the cause of infertility in couples [
4]. Based on 17 annual reports of the EIM between 1997 and 2013, the clinical pregnancy rates per aspiration and per transfer were relatively stable. For IVF, the mean pregnancy rate per transfer is now 34.5% compared with 27.7% in 1999. For intracytoplasmic sperm injection (ICSI), it is 32.9% compared with 27.9% in 1999 [
5,
6]. In spite of some positive changes in the past 20 years, the success rate is still dramatically low. The ultimate goal for treating infertile couple with ARTs is to maximize the number of patients delivering living child. Although these techniques have led to an increase infertility success significantly, attention to additional factors affecting the success of ARTs is also very important and with appropriate interventions in some of these factors, success rate can be as high as improve a lot [
7]. Many studies have been done on the factors influencing the outcomes of infertility treatments and the fundamental role of factors such as female age, number of oocytes transferred, quality of sperm, overweight and obesity has been proven [
8‐
11]. Lifestyle factors are the modifiable behaviours and circumstances of life that can greatly contribute to overall health and subfertility. The impact of lifestyle on ARTs outcomes may vary depending on person aetiology and circumstances. There is a lot of evidence that lifestyle factors can influence on ARTs outcomes [
12,
13]. For example, studies have demonstrated that female age [
14], smoking [
15], weight [
16], diet [
17], exercise [
18], psychological stress [
19], caffeine consumption [
20], alcohol consumption [
20] and exposure to environmental pollutants [
21] significantly decreases the chance of clinical pregnancy and live birth. Little has been done on the effects of male factors on the outcome of ARTs, and clinical observations in this field are small and contradictory. Several studies have shown that overweight and obesity in men lead to changes in hormone levels, and these changes are shown not only in the levels of testosterone and estrogen, but also on sex-hormone binding [
22]. There is strong evidence of the adverse effects of smoking on ARTs outcomes through a range of pathways in both in women and in infertile men. There is a strong association between the number of smoking years during the women’s life time and her risk of not conceiving through IVF [
15]. Current knowledge of the effect of combined effect of several lifestyle factors on the outcomes of ART is low and contradictory. The importance of the causality of this phenomenon is felt by the pandemic of inappropriate lifestyle as well as the dramatic increase in infertility in the world. The aim of this cohort study is to scrutinize the impact of a specific range of contemporary lifestyle factors on ARTs outcomes. The cohort study focuses on the non-communicable aetiology for ARTs outcomes associated with potentially interventable lifestyle factors. These factors include tobacco usage, drugs, weight, physical activity, alcohol drinking and caffeine consumption.