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Erschienen in: Journal of Cancer Research and Clinical Oncology 11/2016

31.08.2016 | Original Article – Clinical Oncology

The crucial role of emilin 1 gene expression during progression of tumor growth

verfasst von: Miroslava Rabajdova, Peter Urban, Ivana Spakova, Ladislav Saksun, Rastislav Dudic, Alexander Ostro, Martin Caprnda, Peter Kruzliak, Mariusz Adamek, Maria Marekova

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 11/2016

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Abstract

Background

This study describes the effect of rapid tumor growth of patients suffering from various grades of malignant ductal breast carcinoma associated with the gene expression of ECM protein emilin 1, in correlation with the number of gene copies of emilin 1 and degradation of tumor tissue proteins.

Methods

A total of 40 examined patients participated in the experiment (controls, n = 10, grades GI–GIII, each n = 10). After isolation of total mRNA, transcription of mRNA into the cDNA was performed. Quantification of gene expression changes was detected by the real-time PCR method. Analysis at the protein level was performed via Western blot method.

Results

During the detection of changes at the mRNA level, a significantly decreased level of emilin 1 in tumor tissues with grade II (about 54 ± 8 % lower than control) was identified. Protein-level analysis indicated an increased level of emilin 1 in tumors with grade I in comparison with control samples (about 10 ± 3 %).

Conclusion

Obtained results demonstrated that the suppressive role of emilin 1 is related to the grade of growing breast tumors, and associated with increased hypoxia in the tumor microenvironment followed by elevated unfolding and degradation of tissue proteins.
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Metadaten
Titel
The crucial role of emilin 1 gene expression during progression of tumor growth
verfasst von
Miroslava Rabajdova
Peter Urban
Ivana Spakova
Ladislav Saksun
Rastislav Dudic
Alexander Ostro
Martin Caprnda
Peter Kruzliak
Mariusz Adamek
Maria Marekova
Publikationsdatum
31.08.2016
Verlag
Springer Berlin Heidelberg
Erschienen in
Journal of Cancer Research and Clinical Oncology / Ausgabe 11/2016
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI
https://doi.org/10.1007/s00432-016-2226-0

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