Skip to main content
Erschienen in: Rheumatology International 6/2014

01.06.2014 | Short Communication

The cytospin technique improves the detection of calcium pyrophosphate crystals in synovial fluid samples with a low leukocyte count

verfasst von: Christoph Robier, Franz Quehenberger, Manfred Neubauer, Mariana Stettin, Franz Rainer

Erschienen in: Rheumatology International | Ausgabe 6/2014

Einloggen, um Zugang zu erhalten

Abstract

In synovial fluids (SF) with low leukocyte or/and crystal counts, important features may be missed, if exclusively smears are examined by polarized microscopy. That may be overcome by cytocentrifuges, which use low-speed centrifugal force to concentrate cells onto a glass slide and thus enhance the number of cells per high power field (HPF). We compared the calcium pyrophosphate (CPP) crystal counts in cytospin preparations with those in common smears of SF. The number of CPP crystals was counted in 50 SF samples by polarized microscopy, and statistical comparisons of the mean values of the cytospin and smear preparations were performed using the Wilcoxon test. The reproducibility within the slides of the cytocentrifuge and smear samples was determined by Spearman’s rank correlation. The crystal counts were significantly higher in the cytospin than in the smear preparations (median 96/10 HPF vs. 2.5/10 HPF, p < 0.0001). The correlation in the crystal count between the slides 1 and 2 was significantly higher within the cytocentrifuge than in the smear group (0.97 vs. 0.73, p = 0.0004). CPP-negative cytospin preparations in initially smear-positive slides were not observed. We confirmed that the cytospin technique significantly enhances the number of examinable crystals per HPF, compared to common smears.
Literatur
1.
Zurück zum Zitat Swan A, Amer H, Dieppe P (2002) The value of synovial fluid assays in the diagnosis of joint disease: a literature survey. Ann Rheum Dis 61:493–498PubMedCentralPubMedCrossRef Swan A, Amer H, Dieppe P (2002) The value of synovial fluid assays in the diagnosis of joint disease: a literature survey. Ann Rheum Dis 61:493–498PubMedCentralPubMedCrossRef
2.
Zurück zum Zitat Zhang W, Doherty M, Bardin T, Barskova V, Guerne P-A, Jansen TL, Leeb BF, Perez-Ruiz F, Pimentao J, Punzi L, Richette P, Sivera F, Uhlig T, Watt I, Pascual E (2011) European league against rheumatism recommendations for calcium pyrophosphate deposition. Part I: terminology and diagnosis. Ann Rheum Dis 70:563–570PubMedCrossRef Zhang W, Doherty M, Bardin T, Barskova V, Guerne P-A, Jansen TL, Leeb BF, Perez-Ruiz F, Pimentao J, Punzi L, Richette P, Sivera F, Uhlig T, Watt I, Pascual E (2011) European league against rheumatism recommendations for calcium pyrophosphate deposition. Part I: terminology and diagnosis. Ann Rheum Dis 70:563–570PubMedCrossRef
4.
Zurück zum Zitat Martinez Sanchis A, Pascual E (2005) Intracellular and extracellular CPPD crystals are a regular feature in synovial fluid from uninflamed joints of patients with CPPD related arthropathy. Ann Rheum Dis 64:1769–1772PubMedCrossRef Martinez Sanchis A, Pascual E (2005) Intracellular and extracellular CPPD crystals are a regular feature in synovial fluid from uninflamed joints of patients with CPPD related arthropathy. Ann Rheum Dis 64:1769–1772PubMedCrossRef
5.
Zurück zum Zitat Kerolus G, Clayburne G, Schumacher HR Jr (1989) Is it mandatory to examine synovial fluids promptly after arthrocentesis? Arthritis Rheum 32(3):271–278PubMed Kerolus G, Clayburne G, Schumacher HR Jr (1989) Is it mandatory to examine synovial fluids promptly after arthrocentesis? Arthritis Rheum 32(3):271–278PubMed
7.
Zurück zum Zitat Von Essen R, Hölttä AMH, Pikkarainen R (1998) Quality control of synovial fluid crystal identification. Ann Rheum Dis 57:107–109CrossRef Von Essen R, Hölttä AMH, Pikkarainen R (1998) Quality control of synovial fluid crystal identification. Ann Rheum Dis 57:107–109CrossRef
8.
Zurück zum Zitat Robier C, Neubauer M, Stettin M, Lunzer R, Rainer F (2012) Dried cytospin preparations of synovial fluid are a stable material for long-time storage and delayed crystal analysis. Clin Rheumatol 31:1115–1116PubMedCrossRef Robier C, Neubauer M, Stettin M, Lunzer R, Rainer F (2012) Dried cytospin preparations of synovial fluid are a stable material for long-time storage and delayed crystal analysis. Clin Rheumatol 31:1115–1116PubMedCrossRef
9.
Zurück zum Zitat McGill NW, York HF (1991) Reproducibility of synovial fluid examination for crystals. Aust N Z J Med 21(5):710–713PubMedCrossRef McGill NW, York HF (1991) Reproducibility of synovial fluid examination for crystals. Aust N Z J Med 21(5):710–713PubMedCrossRef
10.
Zurück zum Zitat Swan A, Heywood B, Chapman B, Seward H, Dieppe P (1995) Evidence for a causal relationship between the structure, size, and load of calcium pyrophosphate dihydrate crystals, and attacks of pseudogout. Ann Rheum Dis 54:825–830PubMedCentralPubMedCrossRef Swan A, Heywood B, Chapman B, Seward H, Dieppe P (1995) Evidence for a causal relationship between the structure, size, and load of calcium pyrophosphate dihydrate crystals, and attacks of pseudogout. Ann Rheum Dis 54:825–830PubMedCentralPubMedCrossRef
11.
Zurück zum Zitat Robier C, Neubauer M, Quehenberger F, Rainer F (2011) Coincidence of calcium pyrophosphate and monosodium urate crystals in the synovial fluid of patients with gout determined by the cytocentrifugation technique. Ann Rheum Dis 70(6):1163–1164PubMedCrossRef Robier C, Neubauer M, Quehenberger F, Rainer F (2011) Coincidence of calcium pyrophosphate and monosodium urate crystals in the synovial fluid of patients with gout determined by the cytocentrifugation technique. Ann Rheum Dis 70(6):1163–1164PubMedCrossRef
12.
Zurück zum Zitat Jaccard YB, Gerster JC, Calame L (1996) Mixed monosodium urate and calcium pyrophosphate crystal-induced arthropathy. A review of 17 cases. Rev Rhum Engl Ed 63:331–335PubMed Jaccard YB, Gerster JC, Calame L (1996) Mixed monosodium urate and calcium pyrophosphate crystal-induced arthropathy. A review of 17 cases. Rev Rhum Engl Ed 63:331–335PubMed
13.
Zurück zum Zitat Liu K, Dodge R, Glasgow BJ, Layfield LJ (1998) Fine needle aspiration: comparison of smear, cytospin, and cell block preparations in diagnostic and cost effectiveness. Diagn Cytopathol 19(1):70–74PubMedCrossRef Liu K, Dodge R, Glasgow BJ, Layfield LJ (1998) Fine needle aspiration: comparison of smear, cytospin, and cell block preparations in diagnostic and cost effectiveness. Diagn Cytopathol 19(1):70–74PubMedCrossRef
14.
Zurück zum Zitat Shah P, Deshmukh R (2012) Exfoliative cytology and cytocentrifuge preparation of oral premalignant and malignant lesions. Acta Cytol 56(1):68–73PubMedCrossRef Shah P, Deshmukh R (2012) Exfoliative cytology and cytocentrifuge preparation of oral premalignant and malignant lesions. Acta Cytol 56(1):68–73PubMedCrossRef
15.
Zurück zum Zitat Hasselbacher P (1987) Variation in synovial fluid analyses by hospital laboratories. Arthr Rheum 30:637–642CrossRef Hasselbacher P (1987) Variation in synovial fluid analyses by hospital laboratories. Arthr Rheum 30:637–642CrossRef
16.
Metadaten
Titel
The cytospin technique improves the detection of calcium pyrophosphate crystals in synovial fluid samples with a low leukocyte count
verfasst von
Christoph Robier
Franz Quehenberger
Manfred Neubauer
Mariana Stettin
Franz Rainer
Publikationsdatum
01.06.2014
Verlag
Springer Berlin Heidelberg
Erschienen in
Rheumatology International / Ausgabe 6/2014
Print ISSN: 0172-8172
Elektronische ISSN: 1437-160X
DOI
https://doi.org/10.1007/s00296-013-2689-0

Weitere Artikel der Ausgabe 6/2014

Rheumatology International 6/2014 Zur Ausgabe

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.