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Journal of Clinical Immunology

Erschienen in:

01.05.2013 | Original Research

The DC-SIGNR 7/5 Genotype is Associated with High Dendritic Cell Counts and Their Subsets in Patients Infected with HIV-1

verfasst von: Omkar Chaudhary, Manju Bala, Jasbir Singh, Anjali Hazarika, Rajesh Kumar, Kalpana Luthra

Erschienen in: Journal of Clinical Immunology | Ausgabe 4/2013

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Abstract

Purpose

The aim of this study was to assess peripheral blood dendritic cell (DC) frequencies and Dendritic Cell-specific intracellular adhesion molecule 3 grabbing non-integrin related (DC-SIGNR) genotyping in healthy individuals, injecting drug users and HIV-1 infected individuals and correlate with different clinical parameters from north India.

Methods

Blood from 30 seronegative healthy individuals, 30 injecting drug users, and 30 patients infected with HIV-1 from North India were collected. Peripheral blood DC frequencies were determined by flow cytometry and repeat region polymorphism in DC-SIGNR was performed by PCR.

Results

There was a significantly lower number of DCs and their subsets in patients infected with HIV-1 compared to injecting drug users and healthy individuals. A significant positive correlation of DCs and their subsets with CD4⁺ T cells and negative correlation with HIV-1 viral load was found. A salient finding of this study was the association of the heterozygous 7/5 DC-SIGNR genotypes with higher percentage of DCs and their subsets and higher CD4⁺ T cell counts and lower viral load compared to the homozygous 7/7 DC-SIGNR genotypes in patients infected with HIV-1.

Conclusions

This is the first study to assess the DC subsets and its association with DC-SIGNR polymorphism in injecting drug users and HIV-1 infected patients and suggests the protective role of 7/5 DC-SIGNR genotypes in HIV-1 infection.

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Titel: The DC-SIGNR 7/5 Genotype is Associated with High Dendritic Cell Counts and Their Subsets in Patients Infected with HIV-1
verfasst von: Omkar Chaudhary
Manju Bala
Jasbir Singh
Anjali Hazarika
Rajesh Kumar
Kalpana Luthra
Publikationsdatum: 01.05.2013
Verlag: Springer US
Erschienen in: Journal of Clinical Immunology / Ausgabe 4/2013
Print ISSN: 0271-9142
Elektronische ISSN: 1573-2592
DOI: https://doi.org/10.1007/s10875-013-9868-3

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The DC-SIGNR 7/5 Genotype is Associated with High Dendritic Cell Counts and Their Subsets in Patients Infected with HIV-1

Abstract

Purpose

Methods

Results

Conclusions

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Abstract

Purpose

Methods

Results

Conclusions

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