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International Journal of Clinical Oncology

Erschienen in:

11.08.2023 | Original Article

The diagnostic potential of two exosome-derived circRNAs for papillary thyroid cancer

verfasst von: Lei Dai, Weibin Hu, Han Jiang, Yingchun Wang, Qi Le, Xianjiang Wu, Xiaodan Meng

Erschienen in: International Journal of Clinical Oncology | Ausgabe 11/2023

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Abstract

Background

As a critical component of exosomes, circular RNAs (circRNAs) have shown great value in cancer diagnosis. This study aimed to identify circRNAs in exosomes for the diagnosis of PTC (papillary thyroid carcinoma).

Methods

We selected hsa_circ_0082002 and hsa_circ_0003863 based on circRNA microarray. The levels of exosomal hsa_circ_0082002 and hsa_circ_0003863 in the sera of healthy control (n = 68), benign thyroid tumors (n = 60), and PTC without and with Hashimoto’s thyroiditis (n = 164) were quantified by qPCR (quantitative polymerase chain reaction). Receiver operating characteristic analyses were conducted to evaluate the diagnostic sensitivity and specificity. Bioinformatics databases were used to predict the microRNAs and proteins binding with hsa_circ_0082002 and hsa_circ_0003863.

Results

The levels of exosomal hsa_circ_0082002 and hsa_circ_0003863 were positively associated and statistically increased in PTC compared to healthy and benign thyroid tumors. Intriguingly, higher levels of exosomal hsa_circ_0082002 and hsa_circ_0003863 were positively correlated with lymph node metastasis and vascular invasion in PTC. Further stability tests show that exosomal hsa_circ_0082002 and hsa_circ_0003863 could exist stably in sera treated by several freeze–thaw cycles at -20 °C and with a storage time shorter than 24 h at 4 °C. Furthermore, hsa_circ_0082002 and hsa_circ_0003863 were predicted to interact with microRNAs and proteins, suggesting that hsa_circ_0082002 and hsa_circ_0003863 might contribute to the occurrence and progression of PTC through interacting with microRNAs and RNA binding proteins.

Conclusion

Collectively, we identified two PTC-related circRNAs incorporated in exosomes and uncovered their potential as tumor markers to diagnose PTC, in particular, more aggressive PTC.

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Titel: The diagnostic potential of two exosome-derived circRNAs for papillary thyroid cancer
verfasst von: Lei Dai
Weibin Hu
Han Jiang
Yingchun Wang
Qi Le
Xianjiang Wu
Xiaodan Meng
Publikationsdatum: 11.08.2023
Verlag: Springer Nature Singapore
Erschienen in: International Journal of Clinical Oncology / Ausgabe 11/2023
Print ISSN: 1341-9625
Elektronische ISSN: 1437-7772
DOI: https://doi.org/10.1007/s10147-023-02400-3

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The diagnostic potential of two exosome-derived circRNAs for papillary thyroid cancer