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01.12.2012 | Primary research | Ausgabe 1/2012 Open Access

Cancer Cell International 1/2012

The differential expression pattern of the BMI-1, SALL4 and ABCA3 genes in myeloid leukemia

Zeitschrift:
Cancer Cell International > Ausgabe 1/2012
Autoren:
Qi Shen, Sichu Liu, Junyan Hu, Shaohua Chen, Lijian Yang, Bo Li, Xiuli Wu, Yu Ma, Jianchang Yang, Yupo Ma, Yangqiu Li
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1475-2867-12-42) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no potential conflicts of interest.

Authors’ contributions

YQL and YPM contributed to concept development and study design. QS, SCL, SHC and YM performed the real-time PCR. JYH, LJY, BL, XLW, JCY were responsible for collection of clinical data. YQL and QS coordinated the study and helped drafting the manuscript. All authors read and approved the final manuscript.

Abstract

Background and methods

In order to characterize the expression pattern of SALL4, BMI-1 and ABCA3 genes in patients with myeloid leukemia and those who achieved complete remission (CR) after chemotherapy. Real-time PCR was used to determine the expression level of these genes in peripheral blood mononuclear cells from 24 patients with AML, eight patients with AML-CR, 13 patients with CML in the chronic phase (CML-CP), 12 patients with CML in blast crisis (CML-BC), 13 patients with CML-CR and 11 healthy individuals (HI).

Results

Overexpression of the BMI-1 gene was found in the AML, CML-CP and CML-BC groups as compared with HI group, while the BMI-1 expression level was lower in patients who achieved CR. In contrast, significantly increased SALL4 expression was only found in AML group, additionally, SALL4 expression was lower in the CML-CP and CML-CR groups compared with the HI group, while the SALL4 expression level in the CML-BC group was higher and significantly greater than that in the CML-CP and CML-CR groups. Moreover, a positive correlation between the expression of SALL4 and BMI-1 genes was found in samples from most groups. There was no significant difference of ABCA3 expression level in AML and CML-BC group in comparison with HI group. Interestingly, the ABCA3 expression level was significantly decreased in the CML-CP, AML-CR and CML-CR in comparison with the HI group. Moreover, the ABCA3 expression level in all of the CR groups was lower than that in their corresponding groups.

Conclusions

These results describe the altered SALL4, ABCA3 and BMI-1 expression pattern in different phases of myeloid leukemia, which may relate to the development and progression to different diseases. SALL4 expression was strongly correlated with BMI-1 in most of the myeloid leukemia patient groups, providing a potential link between SALL4 and BMI-1 in leukemogenesis.
Zusatzmaterial
Authors’ original file for figure 1
12935_2012_370_MOESM1_ESM.tiff
Authors’ original file for figure 2
12935_2012_370_MOESM2_ESM.pdf
Authors’ original file for figure 3
12935_2012_370_MOESM3_ESM.pdf
Authors’ original file for figure 4
12935_2012_370_MOESM4_ESM.pdf
Literatur
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