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Erschienen in: Hepatology International 4/2016

26.02.2016 | Original Article

The dynamic changes of circulating invariant natural killer T cells during chronic hepatitis B virus infection

verfasst von: Man Li, Zhen-Hua Zhou, Xue-Hua Sun, Xin Zhang, Xiao-Jun Zhu, Shu-Gen Jin, Yun Jiang, Ya-Ting Gao, Cheng-Zhong Li, Yue-Qiu Gao

Erschienen in: Hepatology International | Ausgabe 4/2016

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Abstract

Aim

The protective role of invariant natural killer T cells (iNKTs) against hepatitis B virus (HBV) infection remains controversial. We sought to clarify the role of peripheral iNKT cells during chronic HBV infection.

Methods

Sixty patients with chronic HBV infection were categorized into an immune tolerance phase (HBV-IT) (n = 16), an immune clearance phase (HBV-IC) (n = 19) and an inactive carrier phase (HBV-IA) (n = 25). Twenty healthy individuals were enrolled as healthy controls. Another 21 HBeAg-positive patients were administrated with entecavir (0.5 mg/day) for 6 months. The percentages of circulating iNKT cells and their IFN-γ and IL-4 expression levels were examined by flow cytometry. The relationships between serum HBV DNA, ALT levels, the percentages of iNKT cells, and their IFN-γ and IL-4 levels were analyzed.

Results

Compared to healthy controls, the percentage of iNKT cells decreased in HBV-IT, but increased in HBV-IC and HBV-IA. Circulating IFN-γ-producing iNKT cells gradually increased, whereas IL-4-producing iNKT cells gradually decreased from HBV-IT stage to HBV-IC and HBV-IA stages. The frequency of iNKT cells and their IFN-γ levels were reversely correlated with viral load. The levels of IL-4 expressed by iNKT cells were positively correlated to viral load and the serum ALT levels. After anti-virus therapy, the percentage of IFN-γ-producing iNKT cells increased while the percentage of IL-4-producing iNKT cells decreased.

Conclusions

During chronic HBV infection, the percentages of peripheral iNKT cells and its cytokines expressions of IFN-γ and IL-4 showed dynamic changes. The expression levels of IFN-γ and IL-4 were correlated with the clearance of HBV and liver injury.
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Metadaten
Titel
The dynamic changes of circulating invariant natural killer T cells during chronic hepatitis B virus infection
verfasst von
Man Li
Zhen-Hua Zhou
Xue-Hua Sun
Xin Zhang
Xiao-Jun Zhu
Shu-Gen Jin
Yun Jiang
Ya-Ting Gao
Cheng-Zhong Li
Yue-Qiu Gao
Publikationsdatum
26.02.2016
Verlag
Springer India
Erschienen in
Hepatology International / Ausgabe 4/2016
Print ISSN: 1936-0533
Elektronische ISSN: 1936-0541
DOI
https://doi.org/10.1007/s12072-015-9650-0

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