Background
Methods
Study subjects
Measurements
Genotyping
Primer name | Sequence | References |
---|---|---|
TLR_299_F | TGA AGA ATT CCG ATT AGC ATA CTT AGA | [17] |
TLR_299_R | CTT TCA ATA GTC ACA CTC ACC AG | In this study |
TLR_399_F | TGA GTT TCA AAG GTT GCT GTT CTC | [17] |
TLR_399_R | GTT TGA ACT CAT GGT AAT AAC ACC | In this study |
CD14-159_F | GCCTCTGACAGTTTATGTAATC | [14] |
CD14-159_R | GTGCCAACAGATGAGGTTCAC | [14] |
Statistical analysis
Results
Characteristics | Number of cases (n) | Mean (Standart deviation) | Range |
---|---|---|---|
Age (Year) | 131 | 36 (12.42) | 18–68 |
Atopic | 96 | 31 (8.72) | 18–57 |
Non-atopic | 35 | 52 (6.57) | 42–68 |
Control | 75 | 43 (7.68) | 23–65 |
Female (Total) | 96 | ||
Atopic | 63 | ||
Non-atopic | 30 | ||
Control | 50 | ||
Male (Total) | 35 | ||
Atopic | 33 | ||
Non-atopic | 5 | ||
Control | 25 | ||
Duration of asthma (Year) | 131 | 4.52 (5.28) | 0–35 |
FEV1 (%) | |||
Asthma | 131 | 68.60 (12.67) | 37–97 |
Control | 75 | 95.70 (14.56) | 90–115 |
Total IgE (kU/I) | |||
Asthma | 131 | 175.77 (220.28) | 4–986 |
Control | 75 | 45.32 (30.25) | 5–89 |
Eosinophil (%) | |||
Asthma | 131 | 3.39 (2.9) | 0–14 |
Control | 75 | 1.10 (0.5) | 0–3 |
Skin prick test (Asthma) | |||
Positive | 96 | ||
Negative | 35 |
Genotypes/Alleles | Asthma patients n (%) | Control group n (%) | p |
---|---|---|---|
CD14-159 | |||
CC | 26 (19.3) | 15 (19.4) | 0.99 (NS)* |
CT | 63 (49.2) | 36 (49.2) | |
TT | 42 (31.5) | 24 (31.4) | |
CC + CT (C allele) | 115(43.9) | 66 (44) | |
TT (T allele) | 147(56.1) | 84 (56) | |
TLR4 299 | |||
AA | 122 (93.1) | 71 (94.7) | 0.77 (NS)* |
GA | 9 (6.9) | 4 (5.3) | |
A allele | 253 (96.6) | 146 (97.3) | |
G allele | 9 (3.4) | 4 (2.7) | |
TLR4 399 | |||
CC | 120 (91.6) | 71 (94.7) | 0.58 (NS)* |
CT | 11 (8.4) | 4 (5.3) | |
C allele | 251 (95.8) | 146 (97.3) | |
T allele | 11 (4.2) | 4 (2.7) |
All asthma patients | p | Atopic | Non-atopic | p | |||
---|---|---|---|---|---|---|---|
(Standart deviation) | Asthmatics (Standart deviation) | Asthmatics (Standart deviation) | |||||
1 | 2 | 3 | (n = 96) | (n = 35) | |||
(n = 19) | (n = 78) | (n = 34) | |||||
FEV1 (%) | 88 | 72 | 51 |
<0.001
| 71 | 62 |
<0.001
|
(6.7) | (4.2) | (6.2) | (12) | (13) | |||
Eosinophil (%) | 3.25 | 4.02 | 3.61 |
<0.001
| 3.84 | 2.2 |
0.008
|
(3) | (3.35) | (2.5) | (3.2) | (1.3) | |||
Total IgE (kU/L) | 170 | 240 | 199 |
0.006
| 223 | 47 |
<0.001
|
(156) | (255) | (244) | (240) | (37) | |||
Log IgE median | 1.9 | 2.02 | 1.7 |
0.006
| 2.1 | 1.5 |
<0.001
|
(0.5) | (0.6) | (0.6) | (0.5) | (0.4) | |||
CD14-159 | |||||||
CC | 1 | 14 | 5 | 0.27 | 20 | 6 | 0.62 |
CT | 5 | 31 | 7 | 43 | 19 | ||
TT (T Allele) | 9 | 21 | 3 | 33 | 10 | ||
CC+CT (CAllele) | 6 | 45 | 12 | 0.10 | 63 | 25 | 0.68 |
TLR4 299 | |||||||
AA | 14 | 60 | 14 | 0.45 | 88 | 34 | 0.44 |
GA | 1 | 6 | 1 | 8 | 1 | ||
TLR4 399 | |||||||
CC | 14 | 58 | 14 | 0.23 | 86 | 34 | 0.29 |
CT | 1 | 8 | 1 | 10 | 1 |
Atopic asthmatics | p | Non-atopic asthmatics | p | |||||
---|---|---|---|---|---|---|---|---|
(Standart deviation) | (Standart deviation) | |||||||
1 | 2 | 3 | 1 | 2 | 3 | |||
(n = 15) | (n = 66) | (n = 15) | (n = 4) | (n = 12) | (n = 19) | |||
FEV1 (%) | 88 | 72 | 51 |
<0.001
| 89 | 69 | 52 |
0.03
|
(6.7) | (4.2) | (6.2) | (4.4) | (5.1) | (4.9) | |||
Eosinophil (%) | 3.25 | 4.02 | 3.61 | 0.73 | 1.50 | 1.93 | 2.43 | 0.46 |
(3) | (3.35) | (2.5) | (0.6) | (1.2) | (1.4) | |||
Total IgE (kU/L) | 170 | 240 | 199 | 0.63 | 63 | 40 | 43 | 0.49 |
(156) | (255) | (244) | (47) | (33) | (38) | |||
Log IgE median | 2 | 2.1 | 2 | 0.75 | 1.7 | 1.4 | 1.5 | 0.49 |
(0.5) | (0.5) | (0.6) | (0.5) | (0.5) | (0.4) | |||
CD14-159 | ||||||||
CC | 1 | 14 | 5 | 0.14 | 1 | 1 | 4 | 0.70 |
CT | 5 | 31 | 7 | 2 | 7 | 10 | ||
TT (T Allele) | 9 | 21 | 3 | 1 | 4 | 5 | ||
CC + CT (C Allele) | 6 | 45 | 12 |
0.049
| 3 | 8 | 14 | 0.82 |
TLR4 299 | ||||||||
AA | 14 | 60 | 14 | 0.92 | 4 | 11 | 19 | 0.41 |
GA | 1 | 6 | 1 | - | 1 | - | ||
TLR4 399 | ||||||||
CC | 14 | 58 | 14 | 0.70 | 4 | 11 | 19 | 0.41 |
CT | 1 | 8 | 1 |
-
| 1 | - |
Multivariate | |||
---|---|---|---|
OR | 95% CI | p | |
Age | − | − | − |
Sex | − | − | − |
Allergic rhinitis | − | − | − |
Allergic dermatitis | 8.79 | 1.81-46.88 |
0.007
|
Severity of asthma | − | − | − |
Eosinophil (%) | 1.54 | 1.23-1.93 |
0.0001
|
Skin-prick test reactivity | 4.90 | 1.64-14.62 |
0.004
|
CD14 -159 | − | − | − |
TLR4 299 | − | − | − |
TLR4 399 | − | − | − |