The effect of CO₂ 9.3 μm short-pulsed laser irradiation in enamel erosion reduction with and without fluoride applications—a randomized, controlled in vitro study

The aim of this in vitro study was to evaluate the protective effect of short-pulsed CO₂ 9.3 μm laser irradiation against erosion in human enamel without and combined with TiF₄ and AmF/NaF/SnCl₂ applications, respectively, as well as compared to the protective effect of these fluoride treatments alone. After polishing, ninety enamel samples (3 × 3mm) were used for 9 different treatment groups: 4% TiF₄ gel (pH 1.5, 24,533 ppm F⁻); AmF/NaF/SnCl₂ rinse (pH 4.5; 500 ppm F⁻, 800 ppm Sn²); CO₂ laser (average power 0.58 W); CO₂ laser (0.58 W) + TiF₄; CO₂ laser (0.58 W) + AmF/NaF/SnCl₂; CO₂ laser (0.69 W); CO₂ laser (0.69 W) + TiF₄; CO₂ laser (0.69 W) + AmF/NaF/SnCl₂; negative control (deionized water). TiF₄ gel was brushed on only once before the first erosive cycling, while samples treated with AmF/NaF/SnCl₂ were daily immersed in 5 ml of the solution before cycling. Laser treatment occurred with a CO₂ laser (wavelength 9.3 μm, pulse repetition rate 100 Hz, pulse duration 14.6 μs/18 μs, average power 0.58 W/0.69 W, fluence 1.9 J/cm²/2.2 J/cm², beam diameter 0.63 mm, irradiation time 10 s, air cooling). TiF₄ was applied only once, while AmF/NaF/SnCl₂ was applied once daily before the erosive challenge. Surface loss (in μm) was measured with optical profilometry immediately after treatment, and after 5 and 10 days of erosive cycling (0.5% citric acid, pH 2.3, 6 × 2 min/day). Additionally, scanning electron microscopy investigations were performed. All application measures resulted in loss of surface height immediately after treatment. After 5 days, significantly reduced surface loss was observed after applying laser irradiation (both power settings) followed by applications of TiF₄ or AmF/NaF/SnCl₂ solution (p < 0.05; 2-way ANOVA and Tukey test) compared to fluoride application alone. After 10 days, compared to after 5 days, a reduced tissue loss was observed in all groups treated with AmF/NaF/SnCl₂ solution. This tissue gain occurred with the AmF/NaF/SnCl₂ application alone and was significantly higher when the application was combined with the laser use (p < 0.05). Short-pulsed CO₂ 9.3 μm laser irradiation followed by additional application of AmF/NaF/SnCl₂ solution significantly reduces the progression of dental enamel erosion in vitro.