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16.03.2019 | Original Article

The effect of combination therapy on critical-size bone defects using non-activated platelet-rich plasma and adipose-derived stem cells

verfasst von: Woonhyeok Jeong, Young Seok Kim, Tai Suk Roh, Eun Hye Kang, Bok Ki Jung, In Sik Yun

Erschienen in: Child's Nervous System | Ausgabe 1/2020

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Abstract

Purpose

Non-activated platelet-rich plasma (nPRP) slowly releases growth factors that induce bone regeneration. Adipose tissue-derived stem cells (ASCs) are also known to induce osteoblast differentiation. In this study, we investigated the combined effect of nPRP and ASC treatment compared with single therapy on bone regeneration.

Methods

Thirty New Zealand white rabbits with 15 × 15 mm2 calvarial defects were randomly divided into four treatment groups: control, nPRP, ASC, or nPRP + ASC groups. For treatment, rabbits received a collagen sponge (Gelfoam®) saturated with 1 ml normal saline (controls), 1 ml non-activated PRP (nPRP group), 2 × 106 ASCs (ASCs group), or 2 × 106 ASCs plus l ml nPRP (nPRP + ASCs group). After 16 weeks, bone volume and new bone surface area were measured, using three-dimensional computed tomography and digital photography. Bone regeneration was also histologically analyzed.

Results

Bone surface area in the nPRP group was significantly higher than both the control and ASC groups (p < 0.001 and p < 0.01, respectively). The percentage of regenerated bone surface area in the nPRP + ASC group was also significantly higher than the corresponding ratios in the control group (p < 0.001). The volume of new bone in the nPRP group was increased compared to the controls (p < 0.05).

Conclusion

Our results demonstrate that slow-releasing growth factors from nPRP did not influence ASC activation in this model of bone healing. PRP activation is important for the success of combination therapy using nPRP and ASCs.
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Metadaten
Titel
The effect of combination therapy on critical-size bone defects using non-activated platelet-rich plasma and adipose-derived stem cells
verfasst von
Woonhyeok Jeong
Young Seok Kim
Tai Suk Roh
Eun Hye Kang
Bok Ki Jung
In Sik Yun
Publikationsdatum
16.03.2019
Verlag
Springer Berlin Heidelberg
Erschienen in
Child's Nervous System / Ausgabe 1/2020
Print ISSN: 0256-7040
Elektronische ISSN: 1433-0350
DOI
https://doi.org/10.1007/s00381-019-04109-z

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