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Erschienen in: International Urogynecology Journal 11/2011

01.11.2011 | Original Article

The effect of l-arginine on bladder dysfunction following ovariectomy in a rabbit model

verfasst von: Shu-Mien Chuang, Yung-Shun Juan, Cheng-Yu Long, Chun-Hsiung Huang, Robert M. Levin, Keh-Min Liu

Erschienen in: International Urogynecology Journal | Ausgabe 11/2011

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Abstract

Introduction and hypothesis

The present study was designed to investigate the effect of nitric oxide precursor, l-arginine, on bladder function following ovariectomy.

Methods

Twenty-eight New Zealand white female rabbits were separated into seven groups. Groups 1 to 6 underwent ovariectomy surgery. Among them, groups 1 and 2 received ovariectomy without treating with l-arginine. Groups 3, 4, 5, and 6 were given high l-arginine diet and were sacrificed 1, 3, 7, and 14 days after ovariectomy, respectively. Group 7 served as the control group. The effects of l-arginine on the contractility of bladder tissues were determined in response to various stimulations. In addition, l-arginine effects on the expression of Rho kinase (ROK), protein kinase C potentiated inhibitor (CPI-17), caldesmon (CaD), and calponin (CaP) were studied by immunoblotting.

Results

Ovariectomy significantly decreases contractile response to all forms of stimulation. Feeding rabbits l-arginine significantly increases contractile response at 1 day following ovariectomy, but the response decreases to the control level by 14 days. Ovariectomy increases the expressions of both isoforms of CaD, CaP, and CPI-17; l-arginine treatment induces ROK underexpression, while CaP is overexpressed in the early few days of ovariectomy but returns to the control level at 2 weeks after ovariectomy.

Conclusions

Ovariectomy appreciably reduced bladder contractility. Treatment with l-arginine reversed the ovariectomy-induced bladder dysfunction. Decreased bladder contractile response was observed in the early days following ovariectomy.
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Metadaten
Titel
The effect of l-arginine on bladder dysfunction following ovariectomy in a rabbit model
verfasst von
Shu-Mien Chuang
Yung-Shun Juan
Cheng-Yu Long
Chun-Hsiung Huang
Robert M. Levin
Keh-Min Liu
Publikationsdatum
01.11.2011
Verlag
Springer-Verlag
Erschienen in
International Urogynecology Journal / Ausgabe 11/2011
Print ISSN: 0937-3462
Elektronische ISSN: 1433-3023
DOI
https://doi.org/10.1007/s00192-011-1468-2

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