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Erschienen in: Journal of Anesthesia 2/2018

20.02.2018 | Original Article

The effect of sevoflurane on retinal angiogenesis in a mouse model of oxygen-induced retinopathy

verfasst von: Hee Young Kim, Seung-Hoon Baek, Seong Wan Baik, Sun Sik Bae, Jung Min Ha, Minkyoung Kim, Gyeong-Jo Byeon, Hye Jin Kim, Hyun-Su Ri, So Hyun Kim

Erschienen in: Journal of Anesthesia | Ausgabe 2/2018

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Abstract

Background

Sevoflurane is commonly used in general anesthesia for premature neonates. The main mechanism of retinopathy of prematurity (ROP) is increased levels of vascular endothelial growth factor (VEGF). For the investigation of sevoflurane’s effect on angiogenesis, the angiogenesis and VEGF expression in the retina were measured after administering sevoflurane in an oxygen-induced retinopathy mice model.

Materials and methods

The mice were divided into the normoxic group (Nc and Ns group; n = 6) and the ROP group (C, Rc, and Rs group; n = 6). Rc group were exposed to 75% oxygen for 5 days beginning on postnatal day (P) 7, and then returned to room air. Age-matched mice in the C group were exposed to room air. To observe angiogenesis of the retina, the mice were sacrificed on P16. The Rs group was exposed to 2 vol% sevoflurane for 2 h on P12, P13, and P14 with 40% oxygen.

Results

The angiogenic area and the spreading distance of vessels on P4 were statistically decreased in the Ns group, compared to the Nc group. The avascular area on P16 was significantly increased and the expression of VEGF was suppressed in the Rs group compared to the Rc group.

Conclusions

Sevoflurane can inhibit retinal angiogenesis via suppressing VEGF expression in an OIR mice model with exposure to relative hypoxia. Nevertheless, it is still difficult to apply the results of this study immediately to humans because of the heterogeneity of responses to sevoflurane.
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Metadaten
Titel
The effect of sevoflurane on retinal angiogenesis in a mouse model of oxygen-induced retinopathy
verfasst von
Hee Young Kim
Seung-Hoon Baek
Seong Wan Baik
Sun Sik Bae
Jung Min Ha
Minkyoung Kim
Gyeong-Jo Byeon
Hye Jin Kim
Hyun-Su Ri
So Hyun Kim
Publikationsdatum
20.02.2018
Verlag
Springer Japan
Erschienen in
Journal of Anesthesia / Ausgabe 2/2018
Print ISSN: 0913-8668
Elektronische ISSN: 1438-8359
DOI
https://doi.org/10.1007/s00540-018-2465-0

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