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27.08.2016 | Original Paper

The Effects of Alpha Boswellic Acid on Reelin Expression and Tau Phosphorylation in Human Astrocytes

verfasst von: Esmat Fathi, Fatemeh Hedayati Katouli, Gholam Hossein Riazi, Marzieh Dehghan Shasaltaneh, Elham Parandavar, Samaneh Bayati, Ali Afrasiabi, Reza Nazari

Erschienen in: NeuroMolecular Medicine | Ausgabe 1/2017

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Abstract

Reelin is an extracellular glycoprotein which contributes to synaptic plasticity and function of memory in the adult brain. It has been indicated that the Reelin signaling cascade participates in Alzheimer’s disease (AD). Besides the neurons, glial cells such as astrocytes also express Reelin protein. While functional loss of astrocytes has been reported to be associated with AD, dysfunction of astrocytic Reelin signaling pathway has not received much attention. Therefore, we investigated the effects of α-boswellic acid (ABA) as one of the major component of Boswellia serrata resin on primary fetal human astrocytes under a stress paradigm as a possible model for AD through study on Reelin cascade. For this aim, we used streptozotocin (STZ), in which from an outlook generates Alzheimer’s hallmarks in astrocytes, and assayed Reelin expression, Tau and Akt phosphorylation as well as reactive oxygen species (ROS) generation and apoptosis in the presences of ABA. Our results indicated that while STZ (100 µM) down-regulated the expression of Reelin, ABA (25 µM) up-regulated its expression (p < 0.01) for 24 h. ABA efficiently reduced hyperphosphorylated Tau (Ser404) in STZ-treated astrocytes (p < 0.01). Furthermore, STZ-induced apoptosis by increasing cleaved caspase three (p < 0.01) and ROS generation (p < 0.01), a further pathological hallmark of Tauopathy. On the other hand, ABA decreased ROS generation and promoted proliferation of astrocytes through elevating Survivin expression (p < 0.01). These results showed that ABA could be considered as a potent therapeutic agent for prevention and decreasing the progression of Alzheimer’s hallmarks in astrocytes; however, more in vivo studies would be needed.

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Titel: The Effects of Alpha Boswellic Acid on Reelin Expression and Tau Phosphorylation in Human Astrocytes
verfasst von: Esmat Fathi
Fatemeh Hedayati Katouli
Gholam Hossein Riazi
Marzieh Dehghan Shasaltaneh
Elham Parandavar
Samaneh Bayati
Ali Afrasiabi
Reza Nazari
Publikationsdatum: 27.08.2016
Verlag: Springer US
Erschienen in: NeuroMolecular Medicine / Ausgabe 1/2017
Print ISSN: 1535-1084
Elektronische ISSN: 1559-1174
DOI: https://doi.org/10.1007/s12017-016-8437-3

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The Effects of Alpha Boswellic Acid on Reelin Expression and Tau Phosphorylation in Human Astrocytes

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