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Journal of Assisted Reproduction and Genetics

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01.11.2010 | Technical Innovations

The effects of different laser pulse lengths on the embryo biopsy procedure and embryo development to the blastocyst stage

verfasst von: Tyl H. Taylor, Janice W. Gilchrist, Susan V. Hallowell, Kelly K. Hanshew, John J. Orris, Michael J. Glassner, J. David Wininger

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 11/2010

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Abstract

Purpose

A laser is commonly used to remove a blastomere from an embryo for genetic testing. The laser uses intense heat which could possibly disrupt embryo development. It is the goal of this study to test the effects of different laser pulse lengths (and consequently heat) on the embryo biopsy procedure and embryo development.

Methods

Each embryo biopsy was performed randomly utilizing laser pulse lengths of 0.604mS (group I), 0.708mS (group II), and 1.010mS (group III).

Results

For groups I, II, and III, 83, 86, and 71 embryos were biopsied, respectively. There was no difference in day 5 embryo quality or lysed blastomeres between groups. Average number of blastomeres biopsied between group I (1.0 ± 0.0), II (1.0 ± 0.2), and III (1.1 ± 0.2) was significant (0.0001).

Conclusion

Our data demonstrates that laser pulse length does not influence the embryo biopsy procedure or embryo development.

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Titel: The effects of different laser pulse lengths on the embryo biopsy procedure and embryo development to the blastocyst stage
verfasst von: Tyl H. Taylor
Janice W. Gilchrist
Susan V. Hallowell
Kelly K. Hanshew
John J. Orris
Michael J. Glassner
J. David Wininger
Publikationsdatum: 01.11.2010
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 11/2010
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-010-9461-0

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The effects of different laser pulse lengths on the embryo biopsy procedure and embryo development to the blastocyst stage

Abstract

Purpose

Methods

Results

Conclusion

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Springer Medizin

Abstract

Purpose

Methods

Results

Conclusion

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