Erschienen in:
12.08.2019 | Letter to the Editor
The effects of photodynamic therapy with blue light and papain-based gel associated with Urucum, on collagen and fibroblasts: a spectroscopic and cytotoxicity analysis
verfasst von:
Zenildo Santos Silva Jr, Cristiane Miranda França, Renato Araújo Prates, Sergio Brossi Botta, Raquel Agnelli Mesquita Ferrari, Patricia Aparecida Ana, Christiane Pavani, Kristianne Porta Santos Fernandes, Daniela de Fátima Teixeira da Silva, Michael R. Hamblin, Sandra Kalil Bussadori
Erschienen in:
Lasers in Medical Science
|
Ausgabe 3/2020
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Abstract
Papacarie Duo™ is clinically used and has proven effectiveness; however, it is necessary to improve its antimicrobial action. The combined treatment of Papacarie Duo™ with Urucum (Bixa Orellana) could create a potential tool for dental caries treatment; its extract obtained from the seeds’ pericarp contains a water-soluble primary pigment (cis-bixin) with smaller amounts of other carotenoids. The dicarboxylic acid salts of cis-norbixin and trans-norbixin occur in heated alkaline solutions. To analyze the absorption spectra and cytotoxicity (with human dermal fibroblasts) in different concentrations of Urucum, associated or not with Papacarie Duo™, we performed this in vitro study. The effects of pure Urucum, Papacarie Duo™, and PapaUrucum™ on the microstructure of collagen were also analyzed. The application of papain-based gel with Urucum did not present cytotoxicity, its exhibit UV absorption spectrum peak around 460 ± 20 nm. Also, it showed that the compound used did not alter the chemical structure of collagen. Consequently, this product could be used as a chemomechanical method to remove dentin caries as well as being a potential product for antimicrobial photodynamic therapy (aPDT) application.