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16.03.2016 | Original Article

The effects of roflumilast on the pancreas and remote organs in a cerulein-induced experimental acute pancreatitis model in rats

verfasst von: Omer Uslukaya, Ahmet Turkoglu, Umit Can Yazgan, Ibrahim Kaplan, Ibrahim Ibiloglu, Murat Kapan, Metehan Gumus

Erschienen in: Surgery Today | Ausgabe 12/2016

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Abstract

Purpose

Systemic damage in acute pancreatitis (AP) can be characterized by oxidative stress and the release of pro-inflammatory cytokines. Roflumilast has been shown to be a potent anti-inflammatory and antioxidant agent. In the present study, we aimed to investigate the effect of roflumilast in cerulein-induced AP.

Methods

Thirty-two male rats were divided into four groups: group 1 (sham), group 2 (Roflumilast), group 3 (AP), and group 4 (AP + Roflumilast). AP was induced by injecting 4 × 75 μg/kg of body weight at an interval of 1 h. Rats were killed after 12 h following the last cerulein administration. AP was confirmed by measuring the serum amylase level and inflammatory features.

Results

Morphological changes were observed in the pancreas. Amylase levels were higher in the AP and AP + Roflumilast groups than the sham and Roflumilast groups. The serum levels of TNF-α, IL-1β, and IL-6 increased in the AP group, whereas they decreased in the Roflumilast group. The total oxidant activity (TOA) was higher and the total antioxidant capacity (TAC) was lower in the AP group. The administration of roflumilast decreased the TOA and increased the TAC in comparison with the AP group (p < 0.05 for both).

Conclusions

Roflumilast significantly decreases oxidative stress and inflammatory mediators in the plasma, pancreas, and lung in cerulein-induced AP rats.

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Titel: The effects of roflumilast on the pancreas and remote organs in a cerulein-induced experimental acute pancreatitis model in rats
verfasst von: Omer Uslukaya
Ahmet Turkoglu
Umit Can Yazgan
Ibrahim Kaplan
Ibrahim Ibiloglu
Murat Kapan
Metehan Gumus
Publikationsdatum: 16.03.2016
Verlag: Springer Japan
Erschienen in: Surgery Today / Ausgabe 12/2016
Print ISSN: 0941-1291
Elektronische ISSN: 1436-2813
DOI: https://doi.org/10.1007/s00595-016-1329-1

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The effects of roflumilast on the pancreas and remote organs in a cerulein-induced experimental acute pancreatitis model in rats

Abstract

Purpose

Methods

Results

Conclusions

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