Background
Methods
Patients
Immunohistochemistry and scoring
Cell lines
Western blotting
Immunofluorescence
Statistical analysis
Results
CD133 expression was found in the cytoplasm and nucleus in HCC
CD133 (Cytoplasm) | CD133 (Nucleus) | ||||||
---|---|---|---|---|---|---|---|
Variables | No. | Low | High |
p
| Low | High |
p
|
Age (y/o) | |||||||
<65 | 64 | 44 (69) | 20 (31) | 0.485 | 36 (56) | 28 (44) | 0.413 |
≧65 | 55 | 41 (75) | 14 (25) | 35 (64) | 20 (36) | ||
Gender | |||||||
Female | 40 | 33 (83) | 7 (17) | 0.057 | 22 (55) | 18 (45) | 0.461 |
Male | 79 | 52 (66). | 27 (34) | 49 (62) | 30 (38) | ||
Differentiation | |||||||
Undifferentiation | 4 | 3 (75) | 1 (25) | 0.551 | 2 (50) | 2 (50) | 0.703 |
Well | 5 | 5 (100) | 0 (0) | 2 (40) | 3 (60) | ||
Moderate | 55 | 38 (69) | 17 (31) | 31 (56) | 24 (44) | ||
Poor | 53 | 37 (67) | 16 (33) | 35 (66) | 18 (34) | ||
Stage | |||||||
I | 42 | 32 (76) | 10 (24) | 0.429 | 24 (57) | 18 (43) | 0.657 |
II, III | 75 | 52 (69) | 23 (31) | 46 (61) | 29 (39) | ||
Hepatitis B surface antigen | |||||||
Negative | 59 | 42 (71) | 17 (29) | 0.883 | 39 (66) | 20 (34) | 0.163 |
Positive | 58 | 42 (72) | 16 (28) | 31 (53) | 27 (47) | ||
Hepatitis C virus | |||||||
Negative | 75 | 50 (67) | 25 (33) | 0.201 | 41 (55) | 34 (45) | 0.113 |
Positive | 37 | 29 (78) | 8 (22) | 26 (70) | 11 (30) |
Cytoplasmic and nuclear CD133 expression was higher in TU than in AN
The validation of the CD133 antibody (orb18124)
Different effects of OS and RFS on CD133 location of HCC
OS | RFS | ||||||
---|---|---|---|---|---|---|---|
Characteristics | No. | Median survival (days) | Survival (%) | Log-rank | Median survival (days) | Survival (%) | Log-rank |
Age (y/o) | |||||||
<65 | 64 | 1026 | 70.3% | 0.330 | 999 | 67.2% | 0.851 |
≧65 | 55 | 952 | 63.6% | 952 | 63.6% | ||
Gender | |||||||
Female | 40 | 1007 | 70.0% | 0.761 | 1007 | 67.5% | 0.881 |
Male | 79 | 968 | 65.8% | 954 | 64.6% | ||
Differentiation | |||||||
Moderate, Well | 60 | 1047 | 70.0% | 0.354 | 1026 | 70.0% | 0.179 |
Poor, Undifferentiation | 57 | 937 | 64.9% | 1003 | 60.4% | ||
Stage | |||||||
I | 42 | 1035 | 85.7% | 0.003 | 1035 | 85.7% | 0.001 |
II, III | 75 | 934 | 57.3% | 921 | 54.7% | ||
Hepatitis B surface antigen | |||||||
Negative | 59 | 1003 | 69.5% | 0.552 | 982 | 66.1% | 0.861 |
Positive | 58 | 953 | 63.8% | 937 | 63.8% | ||
Hepatitis C virus | |||||||
Negative | 75 | 934 | 62.7% | 0.152 | 934 | 61.3% | 0.189 |
Positive | 37 | 994 | 75.7% | 955 | 73.0% | ||
CD133 (Cytoplasm) | |||||||
Low | 85 | 990 | 72.9% | 0.022 | 990 | 72.9% | 0.043 |
High | 34 | 943 | 52.9% | 944 | 52.9% | ||
CD133 (Nucleus) | |||||||
Low | 71 | 946 | 59.2% | 0.025 | 934 | 56.3% | 0.013 |
High | 48 | 1100 | 79.2% | 1076 | 79.2% |
The location of CD133 is an independent prognostic index for HCC
OS | RFS | |||||||
---|---|---|---|---|---|---|---|---|
Variables | HR | Unfavorable/Favorable |
p
| (95% CI) | HR | Unfavorable/Favorable |
p
| (95% CI) |
CD133 (Cytoplasm) | 2.100 | High/ Low | 0.028 | 1.082–4.075 | 1.946 | High/ Low | 0.046 | 1.012–3.745 |
CD133 (Nucleus) | 2.347 | Low/ High | 0.023 | 1.122–4.907 | 2.550 | Low/ High | 0.012 | 1.228–5.296 |
Stage | 3.092 | II, III/ I | 0.012 | 1.282–7.457 | 3.460 | III, IV/ I, II | 0.005 | 1.441–8.308 |