Introduction
Materials and methods
Tissue samples
Immunohistochemistry
Immunohistochemical evaluations
Statistical analysis
Results
E-cadherin and Snail expression levels
Variables | E-cadherin | Snail (nuclear) | ||||
---|---|---|---|---|---|---|
Preserved (%) | Reduced (%) | p-value | Negative (%) | Positive (%) | p-value | |
Age | 0.80 | 0.14 | ||||
< 50 | 29 (61.7) | 18 (38.3) | 27 (57.4) | 20 (42.6) | ||
≥ 50 | 81 (63.8) | 46 (36.2) | 88 (69.3) | 39 (30.7) | ||
Histology | <0.01 | <0.05 | ||||
Serous | 30 (62.5) | 18 (37.5) | 34 (70.8) | 14 (29.2) | ||
Mucinous | 17 (81.0) | 4 (19.0) | 7 (33.3) | 14 (66.7) | ||
Clear | 16 (50.0) | 16 (50.0) | 23 (71.9) | 9 (28.1) | ||
Endometrioid | 27 (84.4) | 5 (15.6) | 22 (68.8) | 10 (31.2) | ||
SSPC | 13 (50.0) | 13 (50.0) | 17 (65.4) | 9 (34.6) | ||
Others | 7 (46.7) | 8 (53.3) | 12 (80.0) | 3 (20.0) | ||
FIGO stage | 0.39 | 0.68 | ||||
I | 39 (69.6) | 17 (30.4) | 40 (71.4) | 16 (28.6) | ||
II | 9 (64.3) | 5 (35.7) | 10 (71.4) | 4 (28.6) | ||
III | 53 (62.3) | 32 (37.7) | 53 (62.3) | 32 (37.7) | ||
IV | 9 (47.3) | 10 (52.7) | 12 (63.2) | 7 (36.8) | ||
Peritoneal cytology | 0.91 | 0.18 | ||||
Positive | 85 (63.4) | 49 (36.6) | 85 (63.4) | 49(36.6) | ||
Negative | 25 (62.5) | 15 (37.5) | 30 (75.0) | 10 (25.0) | ||
Lymph node metastasis | 0.25 | 0.14 | ||||
Positive | 22 (66.7) | 11 (33.3) | 19 (57.6) | 14 (42.4) | ||
Negative | 60 (67.4) | 29 (32.6) | 65 (73.0) | 24 (27.0) | ||
Nx* | 28 (53.8) | 24 (46.2) | 31 (59.6) | 21 (40.4) | ||
Peritoneal dissemination | 0.09 | 0.10 | ||||
Positive | 54 (57.4) | 40 (42.6) | 57 (60.6) | 37 (39.4) | ||
Negative | 56 (70.0) | 24 (30.0) | 58 (72.5) | 22 (27.5) | ||
Recurrence | <0.01 | 0.73 | ||||
− | 30 (44.1) | 38 (55.9) | 46 (67.6) | 22 (32.4) | ||
+ | 80 (75.5) | 26 (24.5) | 69 (65.1) | 37 (34.9) | ||
End stage | <0.05 | 0.09 | ||||
Alive | 79 (68.7) | 36 (31.3) | 81 (70.4) | 34 (29.6) | ||
Dead | 31 (52.5) | 28 (47.5) | 34 (57.6) | 25 (42.4) |
EMT status
Variables | EMT status (n = 174) | ||
---|---|---|---|
Positive (%) | Negative (%) | p-value | |
Age | 0.41 | ||
< 50 | 11 (22.9) | 37 (77.1) | |
≥ 50 | 22 (17.5) | 104 (82.5) | |
Histology | 0.11 | ||
Serous | 8 (16.7) | 40 (83.3) | |
Mucinous | 3 (14.3) | 18 (85.7) | |
Clear | 11 (34.4) | 21 (65.6) | |
Endometrioid | 2 (6.2) | 30 (93.8) | |
SSPC | 6 (23.1) | 20 (76.9) | |
Others | 3 (20.0) | 12 (80.0) | |
FIGO stage | 0.38 | ||
I | 7 (12.5) | 49 (87.5) | |
II | 4 (28.6) | 10 (71.4) | |
III | 17 (20.0) | 68 (80.0) | |
IV | 5 (26.3) | 14 (73.7) | |
Lymph node metastasis | 0.15 | ||
Positive | 9 (27.3) | 24 (72.7) | |
Negative | 12 (13.5) | 77 (86.5) | |
Nx* | 12 (23.1) | 40 (76.9) | |
peritoneal cytology | 0.12 | ||
Positive | 22 (16.4) | 112 (83.6) | |
Negative | 11 (27.5) | 29 (72.5) | |
Peritoneal metastasis | <0.05 | ||
Positive | 24 (25.5) | 70 (74.5) | |
Negative | 9 (11.3) | 71 (88.7) |
Correlation between the EMT status of the primary tumor and the presence of any corresponding metastases
Variables | Primary tumor | Disseminated tumor | |
---|---|---|---|
n = 34 (%) | n = 34 (%) | P-value | |
E-cadherin | 0.47 | ||
Reduced | 16 (47.1) | 19 (55.9) | |
Preserved | 18 (52.9) | 15 (44.1) | |
Snail | 0.32 | ||
Positive | 11 (32.4) | 15 (44.1) | |
Negative | 23 (67.6) | 19 (55.9) |
Prognostic impact of the EMT status in patients with endometrial carcinoma
Risk | Odds ratio | 95% CI* | p-value |
---|---|---|---|
Lymph node metastasis | 5.14 | 2.15-12.8 | <0.01 |
peritoneal cytology | 3.25 | 1.31-8.93 | <0.05 |
Peritoneal metastasis | 2.19 | 1.01-4.89 | <0.05 |
EMT status | 2.90 | 1.16-7.53 | <0.05 |