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Indian Journal of Otolaryngology and Head & Neck Surgery

Erschienen in:

11.09.2024 | Original Article

The Expressions of p16, HPV16-L1 and HPV18-E6 in Salivary Gland Tumours

verfasst von: Nurul Najwa Mohd Zakir, Norhafiza Mat Lazim, Anani Aila Mat Zin, Ali Haron, Baharudin Abdullah

Erschienen in: Indian Journal of Otolaryngology and Head & Neck Surgery | Ausgabe 6/2024

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Abstract

Aims

This study aimed to investigate the relationship between human papillomavirus (HPV) infection and the development of salivary gland tumour (SGT) by evaluating the expression of p16 and the oncoproteins HPV16-L1 and HPV18-E6 using immunohistochemical (IHC) staining. Additionally, we assess the agreement between these oncoproteins and p16 in diagnosing HPV-infected SGT.

Methods

This cross-sectional study included all SGT cases undergoing surgical resection at Hospital Universiti Sains Malaysia (HUSM) and Hospital Raja Perempuan Zainab II (HRPZ) in Malaysia from April 2022 to April 2023. IHC staining was performed on paraffin-embedded tissues at the Pathology Laboratory, HUSM, to evaluate the expression of p16, HPV16-L1, and HPV18-E6 oncoproteins. The clinicopathological data were correlated with the staining results.

Results

49 SGT cases were identified, mainly in middle-aged Malay women, with most tumours originating from the parotid gland. Malignant tumours included mucoepidermoid carcinoma (22.4%), adenoid cystic carcinoma (4.1%), acinic cell carcinoma (4.1%), and adenocarcinoma (2%). Benign tumours primarily consisted of pleomorphic adenoma (49%) and Warthin tumours (16.3%). Positive p16 expression was detected in 67% of cases, while HPV16 and HPV18 were detected in 65% and 90% of tumours, respectively. HPV16-L1 exhibited 75.8% sensitivity and 56.3% specificity, while HPV18-E6 showed 100% sensitivity and 31.2% specificity compared to p16.

Conclusion

The study findings suggest a correlation between the presence of high-risk HPV types 16 and 18 and the development of SGT, as evidenced by the overexpression of p16, HPV16-L1, and HPV18-E6 oncoproteins. Both HPV16-L1 and HPV18-E6 tests are acceptable, reliable, and sensitive for detecting high-risk HPV in SGT.

Syrjänen K, Syrjänen S, Lamberg M et al (1983) Morphological and immunohistochemical evidence suggesting human papillomavirus (HPV) involvement in oral squamous cell carcinogenesis. Int J Oral Surg 12(6):418–424CrossRefPubMed

Sheedy T, Heaton C (2019) HPV-associated oropharyngeal cancer. JAAPA 32(9):26–31CrossRefPubMed

Tang KD, Vasani S, Menezes L et al (2020) Oral HPV16 DNA as a screening tool to detect early oropharyngeal squamous cell carcinoma. Cancer Sci 111(10):3854–3861CrossRefPubMedPubMedCentral

Mylly M, Nissi L, Huusko T et al (2022) Epidemiological study of p16 incidence in head and neck squamous cell carcinoma 2005–2015 in a representative northern European population. Cancers (Basel) 14(22):5717CrossRefPubMed

Stiasny A, Kuhn C, Mayr D et al (2016) Immunohistochemical Evaluation of E6/E7 HPV oncoproteins staining in Cervical Cancer. Anticancer Res 36:3195PubMed

Azizah A, Hashimah B, Nirmal K et al (2019) Malaysia National cancer registry report (MNCR). National Cancer Institute, Ministry of Health: Putrajaya, Malaysia

Gao M, Hao Y, Huang MX et al (2017) Salivary gland tumours in a northern Chinese population: a 50-year retrospective study of 7190 cases. Int J Oral Maxillofac Surg 46(3):343–349CrossRefPubMed

Saku T, Hayashi Y, Takahara O et al (1997) Salivary gland tumors among atomic bomb survivors, 1950–1987. Cancer 79(8):1465–1475CrossRefPubMed

UNSCEAR 2000 published (2001) United Nations Scientific Committee on the effects of atomic radiation. Health Phys 80(3)

10.

Peter Klussmann J, Wittekindt C, Florian Preuss S et al (2006) High risk for bilateral Warthin tumor in heavy smokers – review of 185 cases. Acta Otolaryngol 126(11):1213–1217CrossRefPubMed

11.

Dong C, Hemminki K (2001) Second primary neoplasms among 53 159 haematolymphoproliferative malignancy patients in Sweden, 1958–1996: a search for common mechanisms. Br J Cancer 85(7):997–1005CrossRefPubMedPubMedCentral

12.

Goldstein AM, Yuen J, Tucker MA (1997) Second cancers after medulloblastoma: Population-based results from the United States and Sweden. Cancer Causes Control 8:865–871CrossRefPubMed

13.

Milán T, Pukkala E, Verkasalo PK et al (2000) Subsequent primary cancers after basal-cell carcinoma: a nationwide study in Finland from 1953 to 1995. Int J Cancer 87:283–288CrossRefPubMed

14.

Hafed L, Farag H, Shaker O, El-Rouby D (2012) Is human papilloma virus associated with salivary gland neoplasms? An in situ-hybridization study. Arch Oral Biol 57:1194–1199CrossRefPubMed

15.

Teng WQ, Chen XP, Xue XC et al (2014) Distribution of 37 human papillomavirus types in parotid gland tumor tissues. Oncol Lett 7:834–838CrossRefPubMed

16.

Qian X, Kaufmann AM, Chen C et al (2016) Prevalence and associated survival of high-risk HPV-related adenoid cystic carcinoma of the salivary glands. Int J Oncol 49:803–811CrossRefPubMed

17.

Brunner M, Koperek O, Wrba F et al (2012) HPV infection and p16 expression in carcinomas of the minor salivary glands. Eur Arch Otorhinolaryngol 269:2265–2269CrossRefPubMed

18.

Miah MS, Majumdar S, White S et al (2015) Human papillomavirus and salivary gland neoplasia: a p16INK4 immunohistochemical and in situ hybridisation study. J Laryngol Otol 129:1000–1003CrossRefPubMed

19.

Haeggblom L, Ursu RG, Mirzaie L et al (2018) No evidence for human papillomavirus having a causal role in salivary gland tumors. Diagn Pathol 13(1):1–5CrossRef

20.

Graham SV (2010) Human papillomavirus: gene expression, regulation and prospects for novel diagnostic methods and antiviral therapies. Future Microbiol 5:1493–1506CrossRefPubMed

21.

Chi AC, Day TA, Neville BW (2015) Oral cavity and oropharyngeal squamous cell carcinoma—an update. CA Cancer J Clin 65(5):401–421CrossRefPubMed

22.

Ball SLR, Winder DM, Vaughan K et al (2011) Analyses of human papillomavirus genotypes and viral loads in anogenital warts. J Med Virol 83:1345–1350CrossRefPubMed

23.

Okunade KS (2020) Human papillomavirus and cervical cancer. J Obstet Gynaecol 40:602–608CrossRefPubMed

24.

Roman A, Munger K (2013) The papillomavirus E7 proteins. Virology 445:138–168CrossRefPubMed

25.

Romagosa C, Simonetti S, López-Vicente L et al (2011) p16Ink4a overexpression in cancer: a tumor suppressor gene associated with senescence and high-grade tumors. Oncogene 30:2087–2097CrossRefPubMed

26.

Augustin JG, Lepine C, Morini A et al (2020) HPV detection in head and neck squamous cell carcinomas: what is the issue? Front Oncol 10

27.

Ndiaye C, Mena M, Alemany L et al (2014) HPV DNA, E6/E7 mRNA, and p16INK4a detection in head and neck cancers: a systematic review and meta-analysis. Lancet Oncol 15(12):1319–1331CrossRefPubMed

28.

Rietbergen MM, Snijders PJF, Beekzada D et al (2014) Molecular characterization of p16-immunopositive but HPV DNA-negative oropharyngeal carcinomas: Characterisation of p16-immunopositive OPSSCs. Int J Cancer 134(10):2366–2372CrossRefPubMed

29.

Ohta S, Uemura H, Matsui Y et al (2009) Alterations of p16 and p14ARF genes and their 9p21 locus in oral squamous cell carcinoma. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 107:81–91CrossRefPubMed

30.

Kim DH, Nelson HH, Wiencke JK et al (2001) P16INK4a and histology-specific methylation of CpG islands by exposure to tobacco smoke in non-small cell lung Cancer1. Cancer Res 61:3419–3424PubMed

31.

Saito Y, Yoshida M, Ushiku T et al (2013) Prognostic value of p16 expression and alcohol consumption in Japanese patients with oropharyngeal squamous cell carcinoma. Cancer 119:2005–2011CrossRefPubMed

32.

Chaturvedi AK (2012) Epidemiology and clinical aspects of HPV in head and neck cancers. Head Neck Pathol 6:16–24CrossRefPubMedCentral

33.

Wang L, Hu Y-C, Xiao C-Y et al (2018) Comparative testing of HPV L1 protein monoclonal antibody panel for the detection of HPV in cervical exfoliated cells. J Virol Methods 257:33–41CrossRefPubMed

34.

Pal A, Kundu R (2020) Human papillomavirus E6 and E7: the cervical cancer hallmarks and targets for therapy. Front Microbiol 10

35.

Lifsics A, Groma V, Cistjakovs M et al (2021) Identification of high-risk human papillomavirus DNA, p16, and E6/E7 oncoproteins in laryngeal and hypopharyngeal squamous cell carcinomas. Viruses 13:1008CrossRefPubMedPubMedCentral

36.

Blatt S, Pabst A, Zimmer S et al (2021) Clinical efficacy of an antibody-based detection system for human papilloma virus infection in oral squamous cell carcinoma. Clin Oral Investig 25:2837–2843CrossRefPubMed

Titel: The Expressions of p16, HPV16-L1 and HPV18-E6 in Salivary Gland Tumours
verfasst von: Nurul Najwa Mohd Zakir
Norhafiza Mat Lazim
Anani Aila Mat Zin
Ali Haron
Baharudin Abdullah
Publikationsdatum: 11.09.2024
Verlag: Springer India
Erschienen in: Indian Journal of Otolaryngology and Head & Neck Surgery / Ausgabe 6/2024
Print ISSN: 2231-3796
Elektronische ISSN: 0973-7707
DOI: https://doi.org/10.1007/s12070-024-05007-w

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Springer Medizin

The Expressions of p16, HPV16-L1 and HPV18-E6 in Salivary Gland Tumours

Abstract